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Showing: 2251 - 2300 of 18693 results

  6. AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

    Plasmid
    #184397
    Purpose
    Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    Firefly luciferase beta-globin-PTC39
    Use
    CRISPR; Donor plasmid for hdr
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    PTC in beta-globin at amino acid position 39
    Promoter
    Tet-On 3G
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184397-plasmid-map-sequence-id-362358

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  14. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69546
    Purpose
    Tet-On system expressing MyoD-2A-GFP-2xNLS.
    Depositor
    Charles Gersbach
    Inserts
    eGFP
    Myod1 (Myod1 Mouse)
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Expression
    Mutation
    Removed stop codon to fuse it to the 2A-eGFPnls i…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69546-plasmid-map-sequence-id-122992

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  32. pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49218
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49218-plasmid-map-sequence-id-73625

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  38. pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A

    Plasmid
    #223560
    Purpose
    Alias: Gαi bONE-GO W139A. Lentiviral vector expressing GINIP-Nluc W139A after a low efficiency IRES downstream of YFP-CAAX placed after the promotor
    Depositor
    Mikel Garcia-Marcos
    Article
    Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457
    Insert
    YFP(Venus) - KRas4b - IRES* - human GINIP W139A - linker(GGGS) - Nluc
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    W139A mutation in GINIP sequence
    Promoter
    CMV
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    223560-plasmid-map-sequence-id-443638

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  43. pCS2-Myc(Δ1-143; ΔDeg1ΔDeg2)-GFP-IRES-mCherry (ΔTADΔDeg1ΔDeg2)

    Plasmid
    #231234
    Purpose
    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    TAD deleted (Δ1-143), deletion of degron 1 & …
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231234-plasmid-map-sequence-id-459167

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  49. pMXS-IRES-BLAST flag-CAD delta Loop1 and Loop2

    Plasmid
    #169833
    Purpose
    Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 and F1366 - P1376 in allosteric domain
    Depositor
    Richard Possemato
    Article
    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422
    Insert
    CAD (CAD Human)
    Use
    Retroviral
    Tags
    Flag tag
    Expression
    Mammalian
    Mutation
    Deletions of amino acids R1398 - S1407 and F1366 …
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    169833-plasmid-map-sequence-id-339176

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  50. pMXS-IRES-BLAST flag-CAD delta Allosteric Domain

    Plasmid
    #169835
    Purpose
    Expresses C-terminal flag-tagged CAD with substitution of allosteric domain F1308 - C1455 with a (GGGS)X3 linker
    Depositor
    Richard Possemato
    Article
    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422
    Insert
    CAD (CAD Human)
    Use
    Retroviral
    Tags
    Flag tag
    Expression
    Mammalian
    Mutation
    Substitution of amino acids F1308 - C1455 with a …
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    169835-plasmid-map-sequence-id-333093

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Showing: 2251 - 2300 of 18693 results