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Plasmid#206373PurposeExpresses EGFP fused mouse SOX17 in mammalian cells, for lentivirus generation.DepositorInsertSox17 (Sox17 Mouse)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_TBA1A
Plasmid#111695PurposeMAC-tagged gene expressionDepositorInsertTBA1A (TUBA1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_minCMV-sfGFP-CMV_dsRed Exp
Plasmid#117401PurposesfGFP expressed from hypoxia-inducible minimal CMV promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC1430
Plasmid#127981PurposeAMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PylRS-AS
Plasmid#137908PurposeFor incorporation of fluorophenylalanine derivativesDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationContaining N346A, C348S mutationsPromoterpBADAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR
Plasmid#161983PurposeAllele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection markerDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS-I997A
Plasmid#111832PurposeVinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vectorDepositorInsertVinculinTS (I997A) (VCL Chicken)
UseLentiviralTagsExpressionMutationchanged vinculin isoleucine 997 to alaninePromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1110
Plasmid#91031PurposeModule A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSPDepositorInsertTaCas9
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-Sox4
Plasmid#36984DepositorInsertSox4 (SOX4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_PDK1
Plasmid#111672PurposeMAC-tagged gene expressionDepositorInsertPDK1 (PDK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0103
Plasmid#91000PurposeModule A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSPDepositorInsertAtCas9
UseCRISPRTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFP.R377
Plasmid#138247PurposeExpresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb713-HO
Plasmid#141199PurposeExpresses mRhubarb713 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb713
heme oxygenase
UseTags6x-HisExpressionBacterialMutationR134H, W309R, Q310A compared to iRFPPromoterT7Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R267
Plasmid#138179PurposeExpresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_211
Plasmid#91110PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
BFP Homology Donor
Plasmid#149372PurposePromoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detectionDepositorInsertCDDR BFP Homology Donor
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-Myc-gate-pGK-HYG
Plasmid#107395PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsMycExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-2xCL-YFP-Vpr
Plasmid#105215PurposeBi-functional Fluorescent reporter protein to visualize HIV-1 fusion and core-traffickingDepositorInsertVpr
UseTagsPC2x (SQNY/IRKVL), eYFP, and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_EPHA2
Plasmid#183286PurposeAll-in-One CRISPRko system with a guide RNA that targets EPHA2 geneDepositorInsertEPHA2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc
Plasmid#80617PurposepBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Crimson)
Plasmid#140162PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termiā¦ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abcde
Plasmid#80619PurposepBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher003-TEVs-SpyTag003DA
Plasmid#216283PurposeExpresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.DepositorInsertSpyCatcher003-TEVs-SpyTag003DA
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Cre-6xHis
Plasmid#62939PurposeExpression of Cre-6xHis in bacterial of cellsDepositorInsertCre recombinase
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK Lentivirus Single Cassette Destination Vector (pAN2414)
Plasmid#194220PurposeBackbone for constructing single casette expression plasmids using the Mammalian TookitDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-SpCas9-NG
Plasmid#171370PurposeSpCas9-NG-mediated genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 for sgRNA; CBh for Cas9-2A-puroRAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abcd
Plasmid#80618PurposepBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1c
Plasmid#80613PurposepBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS-A50I/I997A
Plasmid#111829PurposeVinculin tension sensor with point mutations affecting ability to bind talin and actinDepositorInsertVinculinTS (I997A)
UseTagsExpressionMammalianMutationchanged vinculin alanine 50 to isoleucine and isoā¦PromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_213
Plasmid#91112PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBIG1d
Plasmid#80614PurposepBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1e
Plasmid#80615PurposepBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLP710
Plasmid#203740PurposeIntegration of OsTIR1-3xMyc at NEUT5L locusDepositorInsertOsTIR1
UseCandida albicans integrating vectorTags3xMycExpressionMutationPromoterACT1 (C. albicans)Available sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas11
Plasmid#178882PurposeExpression of human codon-optimized NlaCas11 with N terminal NLS and HA tagDepositorInsertEF1a-NlaCas11c-bGH polyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMGS58
Plasmid#135311PurposeA HygR-P2A-AID cassette to tag the protein of interest with auxin-inducible degron at the n-terminalDepositorInsertHygR-P2A-AID
UseN terminal tagging plasmidTagsExpressionMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry2-sgRNA (empty)
Plasmid#198330PurposeCustomisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporterDepositorInsertU6-sgRNA(F+E) empty
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-V5-hTEV
Plasmid#65800Purposeexpresses human codon-optimised TEV proteaseDepositorInsertV5-human TEV
UseTagsV5ExpressionMammalianMutationcodon-optimised to express in mammalian cellsPromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTCAV-FLEx(loxP)-FlpO
Plasmid#67829PurposeCre-dependent expression of FlpO from CAV2 virusDepositorInsertFlpO
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWXLd-RIEP-GpNLuc
Plasmid#135935PurposeExpresses the GpNLuc LumiFluor in mammalian cells.DepositorInsertGpNLuc LumiFluor
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_DNMT3B
Plasmid#183284PurposeAll-in-One CRISPRko system with a guide RNA that targets DNMT3B geneDepositorInsertDNMT3B
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC_mClover3_BSDminus_F0
Plasmid#184165PurposeDonor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmClover3
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3003
Plasmid#91095PurposeModule C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-mSox17-3xflag
Plasmid#206377PurposeExpresses 3xflag fused mouse SOX17 in mammalian cells, for lentivirus generation.DepositorInsertSox17 (Sox17 Mouse)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R270
Plasmid#138219PurposeExpresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPIDCB2
Plasmid#169900PurposeEmpty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.DepositorTypeEmpty backboneUseAvian expressionTagsExpressionMammalianMutationPromoterDox inducibleAvailable sinceDec. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mut2-RS
Plasmid#182882PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertMut2-RS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAC1749
Plasmid#127983PurposeAMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo*
Plasmid#140159PurposeExpresses a regulatable secretory cargo (without an ER export signal) for mammalian cellsDepositorInsertpiGH-DsRed-Express2-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termiā¦ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only