We narrowed to 4,733 results for: Gca

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pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

Plasmid

#114729

Purpose

3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M5, sgRNA M7, sgRNA M9

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v1 puro

Plasmid

#87790

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-cfTuba_1

Plasmid

#26717

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Tuba RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
non-targeting control gRNA (BRDN0001162235)

Plasmid

#80261

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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PAX6 sgRNA7

Plasmid

#68466

Purpose

targeting PAX6 gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

sgRNA targeting PAX6 (PAX6 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-HEK3-CTTins-px330-scaffold

Plasmid

#180017

Purpose

Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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BRD4 gRNA (BRDN0001146786)

Plasmid

#77346

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD4 (BRD4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIKFYVE gRNA (BRDN0001147303)

Plasmid

#76892

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIKFYVE

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIKFYVE (PIKFYVE Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MET gRNA (BRDN0001147560)

Plasmid

#76061

Purpose

3rd generation lentiviral gRNA plasmid targeting human MET

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MET (MET Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-Control-E2F-3'UTR-Mut

Plasmid

#21171

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

E2F 3'UTR Mutant (E2F1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

2 miR binding sites mutated. Changed from GCACTTT…

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
PTK2 gRNA (BRDN0001146703)

Plasmid

#75544

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PTK2 (PTK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-TBK1-gRNA (PX459)

Plasmid

#221550

Purpose

Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human TBK1 (TBK1 Human)

Expression

Mammalian

Promoter

u6

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

Plasmid

#214879

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF2-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJK01_Rho_minprox_DsREd

Plasmid

#173489

Purpose

PCR template for reporter gene with mouse Rhodopsin promoter with DsRed

Depositor

Barak Cohen

Article

White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Insert

Mouse Rhodopsin promoter driving DsRed reporter gene

Expression

Mammalian

Promoter

Mouse Rhodopsin

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with 8 bp bulge loops (GAPDH)

Plasmid

#170121

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD

Plasmid

#169818

Purpose

Expresses C-terminal flag-tagged CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCCC -> AGTC silent mutations at nt527-530 eli…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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PB09-TRE-beta-globin-miR-124-EF1a-GFP

Plasmid

#117318

Purpose

Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-124-1 (MIR124-1 Human)

Use

Transposon-mediated integration

Tags

GFP

Expression

Mammalian

Promoter

Tet-inducible promoter

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA1

Plasmid

#99734

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-TGFBR2-m3_3'UTR

Plasmid

#31883

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

TGFBR2 mutant 3'UTR (TGFBR2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Third miR-302b, 372 7-mer seed match mutated from…

Promoter

SV40

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

Plasmid

#224697

Purpose

Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)

Tags

Gly-Gly-6xHis

Expression

Bacterial

Mutation

silent mutation C to T at position 10546 (elimina…

Promoter

T7

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pNM58

Plasmid

#128409

Purpose

System with AIP and xylose inducible agrCA sensor

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Inserts

argCA
gfpmut2

Expression

Bacterial

Promoter

P3 and PxylR

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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PB-EEA-g1-PGK-Puro

Plasmid

#102908

Purpose

PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-guide1-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3_shURB5

Plasmid

#81066

Purpose

Gateway entry vector encoding shRNA to UBR5

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

shRNA targeting UBR5

Use

Cloning

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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PMKO-shNC

Plasmid

#64863

Purpose

Expresses a scrambled shRNA

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Insert

Scrambled shRNA

Use

Retroviral

Promoter

u6

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
eGFP L138 gRNA

Plasmid

#119133

Purpose

gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

eGFP L138 gRNA

Use

CRISPR

Mutation

None

Promoter

U6

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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AP568-3

Plasmid

#70048

Purpose

co-expression of Cas9 and crRNA 589 target sequence

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

sgRNA for dpy‐10

Use

CRISPR

Expression

Worm

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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AP568-2

Plasmid

#70047

Purpose

co-expression of Cas9 and crRNA 589 target sequence

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

sgRNA for dpy‐10

Use

CRISPR

Expression

Worm

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pegRNA-HEK3_del1-5-NGG

Plasmid

#185478

Purpose

pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

HEK3_del1-5-NGG pegRNA

Expression

Mammalian

Promoter

U6

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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ACE2 g3

Plasmid

#153013

Purpose

A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

Depositor

Jason Sheltzer

Insert

ACE2 (ACE2 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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px335 Mettl14 sgRNA #1

Plasmid

#61515

Purpose

encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

gcggcagctcctagctcagc

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only