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165,637 results

Showing: 201 - 250 of 165637 results

  1. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Tight TRE promoter
    Available since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

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  2. pLV-EGFP-Cre

    Plasmid
    #86805
    Purpose
    Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter
    Depositor
    Amy Lasek
    Article
    Truitt et al eNeuro. 2016 Oct 31;3(5). pii: ENEURO.02
    Insert
    Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP and nuclear localization signal: PKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    86805-plasmid-map-sequence-id-436570

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  5. pLKO.1 hygro

    Plasmid
    #24150
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
    Depositor
    Bob Weinberg
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for shRNA
    Available since
    March 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    24150-plasmid-map-sequence-id-376525

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  8. pAAV-FLEX-tdTomato (AAV Retrograde)

    Viral Prep
    #28306-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. Cre-dependent (FLEX) tdTomato expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (Cre-dependent)
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  9. PB-TO-hNGN2

    Plasmid
    #172115
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172115-plasmid-map-sequence-id-448148

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  11. pUMVC

    Plasmid
    #8449
    Purpose
    Packaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2005
    Availability
    Academic Institutions and Nonprofits only
    8449-plasmid-map-sequence-id-272509

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  12. pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV9)

    Viral Prep
    #83896-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    dTomato (physically separate, not a fusion protein)
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-syn-dLight1.3b (AAV9)

    Viral Prep
    #135762-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-syn-dLight1.3b (#135762). In addition to the viral particles, you will also receive purified pAAV-syn-dLight1.3b plasmid DNA. Syn-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci
    Promoter
    Syn
    Tags
    None
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  17. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

    Plasmid
    #221359
    Purpose
    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221359-plasmid-map-sequence-id-436820

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  18. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa

    Plasmid
    #221360
    Purpose
    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa; to be used with Anti-V5 [IPI-SV5-Pk1] heavy chain (Plasmid 221359) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221360-plasmid-map-sequence-id-436821

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  20. CMV-Hylight

    Plasmid
    #193447
    Purpose
    A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)
    Depositor
    Richard Goodman
    Article
    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119
    Insert
    Hylight Biosensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    193447-plasmid-map-sequence-id-378450

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  22. pSF3-ultraID

    Plasmid
    #172878
    Purpose
    expresses ultraID in mammalian cells with the tet-on or tet-off system
    Depositor
    Julien Béthune
    Article
    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1
    Insert
    ultraID
    Use
    Luciferase and Retroviral; To be used in a tet-on…
    Tags
    myc
    Expression
    Mammalian
    Mutation
    fragment ([aa2-171]) of aquifex aeolicus BirA wit…
    Promoter
    TRE-cmv
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172878-plasmid-map-sequence-id-340526

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  24. pAAV-hSyn-DIO-EGFP (AAV Retrograde)

    Viral Prep
    #50457-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    Nov. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  35. pAAV.hSynap.iGABASnFR (AAV1)

    Viral Prep
    #112159-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSynap.iGABASnFR (#112159). In addition to the viral particles, you will also receive purified pAAV.hSynap.iGABASnFR plasmid DNA. Synapsin-driven iGABASnFR GABA sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi
    Promoter
    Syn
    Tags
    None
    Available since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only

  36. pLKO.3G

    Plasmid
    #14748
    Depositor
    Diane Mathis, Christophe Benoist
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 5, 2007
    Availability
    Academic Institutions and Nonprofits only
    14748-plasmid-map-sequence-id-352429

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  43. EGFP-GRAM-W

    Plasmid
    #211701
    Purpose
    Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211701-plasmid-map-sequence-id-419225

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  44. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

    Plasmid
    #127289
    Purpose
    all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)
    Depositor
    Fred Gage
    Article
    Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1
    Inserts
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Use
    Lentiviral
    Tags
    Ngn2 and Ascl1 linked via 2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    127289-plasmid-map-sequence-id-308425

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  47. pAAV-hSyn-DIO-mCherry (AAV5)

    Viral Prep
    #50459-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only

  50. pAAV-hsyn-NE2m

    Plasmid
    #208686
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons
    Depositor
    Yulong Li
    Insert
    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    208686-plasmid-map-sequence-id-412172

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Showing: 201 - 250 of 165637 results