We narrowed to 11,308 results for: ena

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4xHRE_YB TATA-sfGFP-CMV_dsRed

Plasmid

#117399

Purpose

sfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBIG2abc

Plasmid

#80617

Purpose

pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG2abcde

Plasmid

#80619

Purpose

pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pUltra-5HTP

Plasmid

#178042

Purpose

Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon

Depositor

Han Xiao

Article

Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

Inserts

Modified ScTrpRS for 5HTP incoporation
suppressor tRNA for 5HTP incorporation

Expression

Bacterial

Mutation

T107C, P254T, C255A

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBIG2abcd

Plasmid

#80618

Purpose

pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only
pBIG1c

Plasmid

#80613

Purpose

pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG1d

Plasmid

#80614

Purpose

pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG1e

Plasmid

#80615

Purpose

pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-sgGOT1

Plasmid

#72874

Purpose

The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgGOT1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#83946

Purpose

Cas9-carrying vector with kanMX marker

Depositor

Irina Borodina

Article

Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

Insert

Cas9

Expression

Yeast

Available Since

Oct. 27, 2016

Availability

Academic Institutions and Nonprofits only

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XPO6-EGFP

Plasmid

#210371

Purpose

Expresses XPO6 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

XPO6 (XPO6 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PDGFRA

Plasmid

#183313

Purpose

All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PDGFRA

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-H-Luc_cyto

Plasmid

#113929

Purpose

Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-17X-PTDH

Plasmid

#166786

Purpose

Phosphite dehydrogenase for NAD(P)H regeneration

Depositor

Wilfred van der Donk

Article

Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

Insert

Phosphite dehydroenase

Tags

His6

Expression

Bacterial

Mutation

A196R, T201S, A328T, E352N, and C356D- please see…

Promoter

T7

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-sfGFP-TET1CD

Plasmid

#184439

Purpose

TET1 catalytic domain fused with sfGFP and scFv against GCN4

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

scFv-GCN4-sfGFP-TET1CD

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-flex-ReaChR-citrine

Plasmid

#50955

Purpose

flexed-ReaChR-citrine in AAV2 vector under human synapsin promoter

Depositor

Roger Tsien

Article

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

AAV

Tags

citrine

Promoter

human synapsin promoter

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb719-HO

Plasmid

#141200

Purpose

Expresses mRhubarb719 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb719
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

Promoter

T7

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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HDM_VSV_G

Plasmid

#204156

Purpose

VSV-G expression plasmid

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

VSV-G

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pX-RCC1-HA

Plasmid

#106938

Purpose

Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

Insert

human RCC1-HA (RCC1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_VPR_yl

Plasmid

#107677

Purpose

CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

Depositor

Ian Wheeldon

Article

Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

Inserts

Codon optimized dCas9-VPR
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_lbMicroID2-HA

Plasmid

#188522

Purpose

Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

lbMicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A1110

Plasmid

#91031

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Promoter

ZmUbi

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_24_positive_Control

Plasmid

#130570

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC1

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMSP3545-dCas9Str

Plasmid

#153516

Purpose

Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

dCas9Str

Expression

Bacterial

Promoter

nisA

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ING2

Plasmid

#13294

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_HDAC3

Plasmid

#183299

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC3

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFP.R270

Plasmid

#138219

Purpose

Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GGG).IMPDH-1.(SIC)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET-SisTnpB1–ωRNA

Plasmid

#228365

Purpose

Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

Depositor

Tao Liu

Article

Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

Insert

SisTnpB1

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCpf1b-sp

Plasmid

#122188

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB179-DepoPETase

Plasmid

#218702

Purpose

Encodes codon optimized DepoPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

DepoPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT1

Plasmid

#183282

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT1

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-HaloTag7-SnoopLigase

Plasmid

#105627

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

HaloTag7-SnoopLigase

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-H2-18 Heavy chain-SpyTag003

Plasmid

#216307

Purpose

Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

H2-18 Heavy chain-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A

Plasmid

#102585

Purpose

Control lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoter

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas3

Plasmid

#178881

Purpose

Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas3c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-ReaChR-citrine

Plasmid

#50956

Purpose

ReaChR-citrine in lentival transfer vector under human synapsin promoter

Depositor

Roger Tsien

Article

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

Lentiviral

Tags

citrine

Promoter

human synapsin promoter

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFP.R269

Plasmid

#138181

Purpose

Expresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(NPC).NrdJ-1.(SEI)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-H-Luc_nucl

Plasmid

#113931

Purpose

Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc_nucl

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas9-J23111

Plasmid

#113149

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Insert

dCas9

Expression

Bacterial

Mutation

D10A & H840A

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)

Plasmid

#194217

Purpose

Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

UAS-ybTATA-tagBFP; PGK-mCitrine

Use

Lentiviral

Promoter

GAL4 UAS-ybTATA; PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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