We narrowed to 15,991 results for: LEA

1. pU6-Lb-crRNA

   Plasmid

   #78957

   Purpose

   Cloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-PANX1(HCVp)

   Plasmid

   #87698

   Purpose

   Express HCV protease-activatible pannexin-1 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   PANX(HCVp)
   

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C1-6xHis

   Plasmid

   #87343

   Purpose

   To express EGFP-6xHis in mammalian cell

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   6xHis
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBad33_ISAam1_reRNA

   Plasmid

   #205322

   Purpose

   Bacterial expression of ISAam1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   ISAam1 TnpB
   

   Expression

   Bacterial

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLew100::NLS-ISceI-HA

   Plasmid

   #21299

   Depositor

   Nina Papavasiliou

   Article

   Boothroyd et al Nature. 2009 May 14;459(7244):278-81. do

   Insert

   I-SceI (SCEI )
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Available Since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4-TO-Hygromycin-sfGFP-MAP

   Plasmid

   #44100

   Depositor

   Dannel McCollum

   Article

   Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

   Type

   Empty backbone

   Tags

   sfGFP-MAP

   Expression

   Mammalian

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCW57-mcGAS

   Plasmid

   #185458

   Purpose

   Inducible FLAG-tagged mouse cGAS

   Depositor

   Shitao Li

   Article

   Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

   Insert

   cGAS
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMA2775

   Plasmid

   #46884

   Purpose

   Lentiviral vector encoding Tet-inducible mitochondrially targeted myc-tagged E.coli exonuclease III

   Depositor

   Mikhail Alexeyev

   Article

   Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

   Insert

   exonucleaseIII E. coli
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   TRE-Tight

   Available Since

   Aug. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAX-SCLM

   Plasmid

   #216757

   Purpose

   For strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoter

   Depositor

   George Augustine

   Insert

   SuperClomeleon
   

   Expression

   Mammalian

   Mutation

   S30R and 11 AA deletion in the Cerulean CFP moiet…

   Promoter

   CAG (cytomegalovirus-chicken beta-actin-rabbit be…

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p8.9 NdeltaSB

    Plasmid

    #132929

    Purpose

    Minimal HIV-1 packaging plasmid for gag and pol expression

    Depositor

    Jeremy Luban

    Article

    Berthoux et al J Virol. 2004 Nov;78(21):11739-50. doi:

    Insert

    gag and pol
    

    Use

    Lentiviral

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. coel::GFP

    Plasmid

    #8937

    Depositor

    Piali Sengupta

    Article

    Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

    Insert

    unc-122 promoter (unc-122 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-survivin

    Plasmid

    #196908

    Purpose

    visualization of midzone on anaphase and midbody later during mitosis in living mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Fukada et al Biotechniques. 2004 Oct;37(4):552, 554,

    Insert

    survivin
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E129K

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC-Kan-alphaGal4

    Plasmid

    #13421

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    RNAP alpha-Gal4
    

    Expression

    Bacterial

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-PRSX8ChR2 (H134R)eYFP

    Plasmid

    #89539

    Purpose

    Expresses ChR2 (H134R) -eYFP in Phox2 (including catecholamine) neurons

    Depositor

    Ruth Stornetta

    Article

    Holloway et al Eur J Neurosci. 2015 Sep;42(6):2271-82.

    Insert

    ChR2 (H134R)
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. ZipGFP11_TEV

    Plasmid

    #81243

    Purpose

    Zipped GFP11 with TEV cleavage sequence

    Depositor

    Xiaokun Shu

    Article

    To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

    Insert

    zipped GFP11 with TEV cleavage sequence
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-RanV19

    Plasmid

    #118316

    Purpose

    Expression of human Ran

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Insert

    RanV19 (RAN Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSL4164 (pEffector_IscB,ωRNA)

    Plasmid

    #210874

    Purpose

    Expresses GstIscB and native ωRNA from constitutive promoters

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    GstIscB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4-TO-Hygromycin-mVenus-MAP

    Plasmid

    #44099

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Tags

    mVenus-MAP

    Expression

    Mammalian

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNLS-LEXY (pDN138)

    Plasmid

    #72657

    Purpose

    golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Type

    Empty backbone

    Use

    Golden gate entry vector

    Tags

    NLS and mCherry-LEXY

    Expression

    Mammalian

    Promoter

    CMV-F

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJH674_pTech_PlldR-HHRz-supP

    Plasmid

    #163728

    Purpose

    Lactate-inducible expression of supP, preceded by a hammerhead ribozyme

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    hhrz-supP
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CMVTO-SEAP-26Sfur-3TM-tevs-KKMP

    Plasmid

    #179634

    Purpose

    TEVP-inducible tri-transmembrane SEAP RELEASE construct

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    SEAP RELEASE
    

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GST-PRMT6

    Plasmid

    #34700

    Depositor

    Steven Clarke

    Article

    Frankel et al J Biol Chem. 2002 Feb 1;277(5):3537-43.

    Insert

    PMRT6
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pSHS212

    Plasmid

    #87346

    Purpose

    Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/C574S

    Promoter

    T7

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDAI-SceI

    Plasmid

    #32061

    Purpose

    Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

    Depositor

    Miguel Valvano

    Article

    Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

    Insert

    I-SceI nuclease
    

    Expression

    Bacterial

    Promoter

    Pdhfr

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. msEGFP-PACR_pcDNA3

    Plasmid

    #55774

    Purpose

    Genetically encoded caged Ca2+

    Depositor

    Takeharu Nagai

    Article

    Fukuda et al ACS Chem Biol. 2014 May 16;9(5):1197-203

    Insert

    PACR
    

    Tags

    msEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 3, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pHAGE-RSV-GCAMP6f

    Plasmid

    #80315

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Darrell Kotton

    Article

    Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

    Insert

    GCaMP6f
    

    Use

    Lentiviral

    Promoter

    RSV

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSLS.436

    Plasmid

    #184953

    Purpose

    Express -Eco1 RT and ncRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: ncRNA(wt) and RT
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HS-Ras-mKate2

    Plasmid

    #105951

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    Ras
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBad33_ISAs26_reRNA

    Plasmid

    #205326

    Purpose

    Bacterial expression of ISAs26 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISAs26 TnpB
    

    Expression

    Bacterial

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-SUMO-AsCas12f

    Plasmid

    #171612

    Purpose

    AsCas12f1 heterologous expression plasmid

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1
    

    Expression

    Bacterial

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEGFP-C1-PRKAA2

    Plasmid

    #30310

    Depositor

    Jay Brenman

    Article

    Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

    Insert

    AMP-activated Protein Kinase α2 (Prkaa1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA3_Gap43_Csx30_Cre

    Plasmid

    #197604

    Purpose

    Cre reporter for mammalian cells with Gap43 membrane anchor

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    Gap43_Csx30_Cre
    

    Expression

    Mammalian

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSL4369 (pEffector_TnpB2,ωRNA)

    Plasmid

    #210871

    Purpose

    Expresses GstTnpB2 and native ωRNA from constitutive promoters

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    GstTnpB2
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYPQ141B-ge4.1

    Plasmid

    #205144

    Purpose

    Guide RNA expression cassette harboring AtU3 promoter, ge4.1 5’ modification, and ge4.1 3’ modification

    Depositor

    Yiping Qi

    Article

    Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

    Insert

    gRNA cloning site
    

    Use

    CRISPR

    Promoter

    AtU3

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CAG.mCherry-eDHFR

    Plasmid

    #176201

    Purpose

    Negative control of targeted optogenetic manipulation using TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    mCherry-eDHFR
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CMVTO-SEAP-26Sfur-3TM-hcvs-KKMP

    Plasmid

    #179635

    Purpose

    HCVP-inducible tri-transmembrane SEAP RELEASE construct

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    SEAP RELEASE
    

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CMVTO-SEAP-26Sfur-B2AD-hcvs-KKMP

    Plasmid

    #179638

    Purpose

    HCVP-inducible single TM SEAP RELEASE construct

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    SEAP RELEASE
    

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA3_Chrm3_Csx30_Cre

    Plasmid

    #197603

    Purpose

    Cre reporter for mammalian cells with Chrm3 membrane anchor

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    Chrm3_Csx30_Cre
    

    Expression

    Mammalian

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMT1002

    Plasmid

    #8621

    Depositor

    Robert Hartley

    Article

    Hartley et al Protein Peptide Letters 1996 3:225-231

    Insert

    barnase, barstar
    

    Tags

    as pMT416

    Expression

    Bacterial

    Mutation

    CI157 lambda repressor gene has two HindIII sites…

    Available Since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

40. pHJ322

    Plasmid

    #20677

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Moesin (MSN Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Thr 558 to Asp

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBSKΔB-CAG-MCP-tdiRFP670-IRES-Neo

    Plasmid

    #62345

    Purpose

    Expresses MCP-tdiRFP670 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    MCP-tdiRFP670
    

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. ZFD_TRAC-NC-Right-G1397-C

    Plasmid

    #180773

    Purpose

    Expresses ZFD_TRAC-NC-Right-G1397-C(DddAtox half) in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

    Insert

    ZFD for TRAC mutation
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. ZFD_TRAC-NC-Left-G1397-N

    Plasmid

    #180772

    Purpose

    Expresses ZFD_TRAC-NC-Left-G1397-N(DddAtox half) in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

    Insert

    ZFD for TRAC mutation
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSL5200 (pDonor_ISGst3)

    Plasmid

    #210875

    Purpose

    Harbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    ISGst3
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. BSP605

    Plasmid

    #122250

    Purpose

    expression vector for mCerulean under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mCerulean
    

    Expression

    Worm

    Promoter

    EFT3

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCRL1

    Plasmid

    #153997

    Purpose

    sgRNA in vitro transcription plasmid.

    Depositor

    Shengdar Tsai

    Article

    Lazzarotto et al Nat Protoc. 2018 Nov;13(11):2615-2642. d

    Type

    Empty backbone

    Use

    CRISPR; Sgrna under a t7 promoter for in vitro tr…

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBad33_ISEc41_reRNA

    Plasmid

    #205341

    Purpose

    Bacterial expression of ISEc41 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISEc41 TnpB
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBad33_ISEc46_reRNA

    Plasmid

    #205343

    Purpose

    Bacterial expression of ISEc46 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISEc46 TnpB
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pBad33_ISNsp2_reRNA

    Plasmid

    #205350

    Purpose

    Bacterial expression of ISNsp2 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISNsp2 TnpB
    

    Expression

    Bacterial

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pDARMO_CMVT_N_FLAG_UBA6

    Plasmid

    #197971

    Purpose

    Mammalian cell expression of FLAG-UBA6

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    UBA6 (UBA6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge