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Showing: 3001 - 3040 of 160228 results

  2. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV8)

    Viral Prep
    #20297-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Cre-dependent)
    Available since
    Dec. 18, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. pCfB2793

    Plasmid
    #64043
    Purpose
    pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.
    Depositor
    Jerome Maury, Irina Borodina
    Article
    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi
    Insert
    GFP
    Use
    Cre/Lox
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 promoter and none
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    64043-plasmid-map-sequence-id-149101

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  6. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

    Plasmid
    #196196
    Purpose
    homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus
    Depositor
    Jürgen Knoblich
    Article
    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75
    Insert
    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
    Use
    Homology repair donor
    Tags
    V5-APEX2-mScarlet-I
    Expression
    Mutation
    Promoter
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    196196-plasmid-map-sequence-id-385065

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  8. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV1)

    Viral Prep
    #20297-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Cre-dependent)
    Available since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  10. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  19. pT3TS-zSpRY

    Plasmid
    #179317
    Purpose
    In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter
    Depositor
    Miguel Angel Moreno-Mateos
    Article
    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:
    Insert
    Zebrafish codon-optimized Cas9 variant SpRY
    Use
    CRISPR; In vitro transcription by t3 promoter
    Tags
    SV40-NLS
    Expression
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…
    Promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    179317-plasmid-map-sequence-id-354723

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  23. ORAI1-YFP V107M

    Plasmid
    #114180
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP V107M (ORAI1 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    V107M
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114180-plasmid-map-sequence-id-218674

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  25. Peripherin/pBSSK+

    Plasmid
    #98132
    Purpose
    Peripherin (NM_006262) in pBSSK+
    Depositor
    Rita Shiang
    Insert
    Peripherin (PRPH Human)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    98132-plasmid-map-sequence-id-190026

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  27. dVGlut-Gal80

    Plasmid
    #52555
    Purpose
    Expresses Gal80 under vesicular glutamate transporter promoter
    Depositor
    Leslie Vosshall
    Insert
    VGlut-Gal80 (VGlut, GAL80 Fly, Budding Yeast)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Drosophila Synthetic Core Promoter
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    52555-plasmid-map-sequence-id-81590

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  32. pHyVec4

    Plasmid
    #34791
    Depositor
    Rob Steele
    Type
    Empty backbone
    Use
    Hydra expression
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Hydra vulgaris actin promoter
    Available since
    Jan. 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    34791-plasmid-map-sequence-id-41829

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  33. CoV2-N-T205I

    Plasmid
    #177954
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (T205I) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T205I
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177954-plasmid-map-sequence-id-348181

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  37. mEmerald-Alpha-Actinin2-N-18

    Plasmid
    #53988
    Depositor
    Michael Davidson
    Insert
    Alpha-Actinin2
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 19, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. pHyVec2

    Plasmid
    #34790
    Depositor
    Rob Steele
    Type
    Empty backbone
    Use
    Hydra expression
    Tags
    Expression
    Mutation
    Promoter
    Hydra vulgaris actin promoter
    Available since
    Jan. 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    34790-plasmid-map-sequence-id-41827

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  40. R6K_tac_GFPmut3_ChInt

    Plasmid
    #187381
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible GFPmut3
    Depositor
    Roi Avraham
    Insert
    GFPmut3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187381-plasmid-map-sequence-id-370413

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Showing: 3001 - 3040 of 160228 results