We narrowed to 5,133 results for: puc
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Plasmid#153251PurposeM8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only
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pDGE497
Plasmid#153249PurposeM6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-22:CETN2-mTagRFP-T
Plasmid#101781PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2DepositorInsertCETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
TC1316
Plasmid#164883PurposeCMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3DepositorInsertdcas13d-ADARdd
ExpressionMammalianMutationdCas13d L560-G586 deletedPromoterCMV, T7Available SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD315 (mTurquoise2^SEC^2xHA)
Plasmid#73343PurposemTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology armsDepositorInsertmTurquoise2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags2xHA and C. elegans codon-optimized mTurquoise2ExpressionWormAvailable SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLTR.gp140/EGFP.Revdelta38/DsRed
Plasmid#115775PurposeHIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.DepositorInsertEnvelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
TagsEGFP; dsReDExpressionMammalianMutationgp140 uncleaved (1190-91aa, KR > TG; truncated…PromoterHIV-1 (LTR)Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry
Plasmid#177182Purposeplasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry markerDepositorInsertpegRNA targeting the PEAR-GFP plasmid
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
TC1614
Plasmid#164873PurposeCURE-X: C to U RNA Editing, with low off-target effects but also lower efficiencyDepositorInserthAPOBEC3A inserted into RfxCas13d (APOBEC3A )
UseCRISPRExpressionMammalianMutationhuman apobec Y132DAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9679-sgRNA-BFP
Plasmid#162988PurposesgRNA cloning plasmidDepositorInsertexchangeable cassette
UseCRISPRExpressionBacterial and MammalianPromoterU6Available SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJBL703
Plasmid#140373PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoterDepositorInsertT7-2BP-tetO-3WJdB-T
UseSynthetic BiologyAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-ZoAl-RTD-3xF-ORF
Plasmid#238486PurposeFor production of C-term flag-tagged ZoAl RT-dead ORF mRNA for mRNA transfection in PRINTDepositorInsertR2 retroelement mRNA
UseIvt templateTags3x-FlagMutationDD644/645->AAAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT7-ZoAl-END-3xF-ORF
Plasmid#238485PurposeFor production of C-term flag-tagged ZoAl EN-dead ORF mRNA for mRNA transfection in PRINTDepositorInsertR2 retroelement mRNA
UseIvt templateTags3x-FlagMutationDD1041/1054->AAAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT7-TaGu-RTD-3xF-ORF
Plasmid#238490PurposeFor production of C-term flag-tagged TaGu RT-dead ORF mRNA for mRNA transfection in PRINTDepositorInsertR2 retroelement mRNA
UseIvt templateTags3x-FlagMutationDD660/661->AAAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT7-TaGu-END-3xF-ORF
Plasmid#238489PurposeFor production of C-term flag-tagged TaGu EN-dead ORF mRNA for mRNA transfection in PRINTDepositorInsertR2 retroelement mRNA
UseIvt templateTags3x-FlagMutationDD1057/1070->AAAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)
Plasmid#164041PurposeCRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B geneDepositorInsertmCherry-CRISPR-TagV2
UseCRISPRExpressionMammalianAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-72:TFAM-mEGFP
Plasmid#159745PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human TFAMDepositorInsertTFAM Homology Arms with linker-mEGFP (TFAM Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceOct. 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDT-sgRNA-XMAS-1x
Plasmid#164413PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mCherry
Plasmid#175507PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3G-mCMVAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLoxP-DHFR-mCherry
Plasmid#70147PurposeExpresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletionDepositorInserta selectable dihydrofolate reductase-thymidylate synthase marker
UseExpression in toxoplasma gondiiTagsmCherryPromoterDHFR 5' UTRAvailable SinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
AA041
Plasmid#215963PurposeFragmid fragment: (Pol 2 promoter) human core (short) EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEFS_v1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPAP_BGL1
Plasmid#183735PurposeExpresses Aspergillus aculeatus β-glucosidase (BGL1)DepositorInsertβ-glucosidase (AaBGL1)
ExpressionYeastPromoterAOX1 promoterAvailable SinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTS2340_Tier3(SB)-iRFP_Puro
Plasmid#169638PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry
Plasmid#177183Purposeplasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry markerDepositorInsertsgRNA targeting the PEAR-GFP plasmid
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
AA294
Plasmid#215968PurposeFragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoterDepositorHas ServiceCloning Grade DNAInsertU1a_v1
UseFragmentAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA332
Plasmid#216053PurposeFragmid fragment: (C' terminus) 3x NLS sequencesDepositorHas ServiceCloning Grade DNAInsertSV40NLS_v1.10; SV40NLS_v1.11; SV40NLS_v1.12
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_mScarlet-I_Donor
Plasmid#173207PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N mScarlet-I donor
UseCRISPRExpressionMammalianPromoterhPGK1Available SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTW072M
Plasmid#115960PurposeDestination VectorDepositorInsertLvl2 (nsP2 Q739L)
UseSynthetic BiologyMutationnsP2 Q739LAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDAS12342_PEAR-GFP_2in1
Plasmid#177184PurposePEAR-GFP plasmid with a pegRNA that targets its own plasmidDepositorInsertsEGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid
UseCRISPRExpressionMammalianMutationdisrupted 5' splice sitePromoterCMV and U6Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRU5-inGFPt
Plasmid#60236PurposeHTLV-1 based transfer vector encoding gfp-turbo reporter gene interrupted by intronDepositorInsertinGFPt
UseRetroviralAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT1420
Plasmid#228316PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT950
Plasmid#228290Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAT9675-CBE
Plasmid#163007Purposeplasmid expressing CBEDepositorInsertCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-BvDODAα1
Plasmid#162528PurposeBeta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertDODAα1
UsePuc19-derivedExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor
Plasmid#178087PurposeHDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertmScarlet-I mTOR signaling indicator (TOSI)
UseCRISPRExpressionMammalianPromoterHuman PGK1Available SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPE9
Plasmid#83545PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.DepositorInsertTEF1 promoter-yEGFP
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAT9748-dCBE
Plasmid#163008Purposeplasmid expressing CBE with dCas9DepositorInsertdCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Consensus
Plasmid#176678PurposeExpression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-078:G3BP1-mEGFP
Plasmid#193920PurposeHomology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1DepositorInsertG3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerMutationhomology arms contain point mutations to disrupt …Available SinceDec. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
p1071-Lenti-Single loxP
Plasmid#217892PurposeLentiviral vector, entry vector for Lentiviral Switch-OVERDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralExpressionMammalianPromoterhU6Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGE476
Plasmid#153267PurposeM2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
EPB104
Plasmid#68369Purposecontains human U6 promoterDepositorAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
AA011
Plasmid#215973PurposeFragmid fragment: (N' terminus) for C>T base editingDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA010
Plasmid#216038PurposeFragmid fragment: (C' terminus) for C>T base editingDepositorHas ServiceCloning Grade DNAInsertUGI_v1.1; NpNLS_v1.1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA013
Plasmid#215975PurposeFragmid fragment: (N' terminus) for A>G base editingDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aalb_743
Plasmid#176662PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
AA302
Plasmid#216052PurposeFragmid fragment: (C' terminus) Nucleoplasmin NLSDepositorHas ServiceCloning Grade DNAInsertNpNLS_v1.2; Stop; polyA_v1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRepair-MPP8_mAID-P2A-BFP
Plasmid#175036PurposeHomology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9DepositorInsertmAID-P2A-BFP
UseHdr donor templateAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-MtPT4Pro
Plasmid#159414PurposeMedicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertMtPT4 Promoter
UsePuc19-derivedExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-MtBCP1Pro
Plasmid#159415PurposeMedicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertMtBCP1 Promoter
UsePuc19-derivedExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRU5-inmCherry
Plasmid#60238PurposeHTLV-1 based transfer vector encoding mcherry reporter gene interrupted by intronDepositorInsertinmCherry
UseRetroviralAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only