We narrowed to 32,438 results for: Eng;
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Plasmid#41819PurposeExpresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineeringDepositorInsertgRNA_GFP-T1
UseCRISPRExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
SL2 GFP H2B
Plasmid#227717PurposePlasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertSL2 GFP H2B (nlp-51 Nematode)
UseCloning vectorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBudORF2-CH
Plasmid#51289Purposeexpresses the codon optimized ORF2 of L1RP with his tagDepositorAvailable SinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT01
Plasmid#223373PurposeT-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.DepositorInsertAtUBQ10-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSH62-EBD
Plasmid#49455Purposeyeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinaseDepositorInsertCre-EBD fusion
UseCre/LoxExpressionYeastPromoterGal1 promoterAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBudORF1-CH
Plasmid#51290Purposeexpresses the codon optimized ORF1 of L1RP with his tagDepositorAvailable SinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
human pirh2-(1-261)
Plasmid#24878DepositorAvailable SinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_Ado1.0
Plasmid#153291PurposeExpress the adenosine sensor GRAB_Ado1.0 in Cre positive cellsDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVPromoterEFSAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT05
Plasmid#223377PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpCas9-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAC1802_pmax-RBFOX1N-dCasRx-C
Plasmid#118635PurposeTransient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoterDepositorInsertRBFOX1N-dCasRx-C
UseCRISPRExpressionMammalianPromoterCAGGSAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Sox10_enh_99a_Citrine
Plasmid#130589Purposeenhancer reporterDepositorInsertSox10_enh_99a
UseEnhancer reporterPromoterthymidine kinase minimal promoterAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_TFAP2b_enh_226_Citrine
Plasmid#130618Purposeenhancer reporterDepositorInsertTFAP2b_enh_226
UseEnhancer reporterPromoterthymidine kinase minimal promoterAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI5-CasRx-HA (pKW88)
Plasmid#229046PurposeMammalian expression of CasRx-HADepositorInsertCasRx (RfxCas13d)
UseCRISPRTagsHAExpressionMammalianAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT10
Plasmid#223382PurposeT-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-SpRY-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTK_Msx1_enh_264_Citrine
Plasmid#130610Purposeenhancer reporterDepositorInsertMsx1_enh_264
UseEnhancer reporterPromoterthymidine kinase minimal promoterAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHT-trxA.ads
Plasmid#47383PurposeExpresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteriaDepositorInsertads
Tags6xHis and trxAExpressionBacterialPromoterPgracAvailable SinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTK_TFAP2a_enh_249_Cerulean
Plasmid#130615Purposeenhancer reporterDepositorInsertTFAP2a_enh_249
UseEnhancer reporterPromoterthymidine kinase minimal promoterAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Axud1_enh_394_Citrine
Plasmid#130625Purposeenhancer reporterDepositorInsertAxud1_enh_394
UseEnhancer reporterPromoterthymidine kinase minimal promoterAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-jGCaMP7b-XC
Plasmid#178361PurposeImproved genetic encoded calcium sensor based on GCaMP7b.DepositorInsertjGCaMP7b-XC
ExpressionMammalianAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-TagBFP-2A-BSD
Plasmid#167929PurposeLentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6, EF1aAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCW01
Plasmid#72300PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19ExpressionBacterialPromoterlacAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAct-Cas9
Plasmid#62209PurposeExpresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.DepositorInsertcas9
ExpressionInsectPromoteract5CAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T1
Plasmid#41817PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T1
UseCRISPRExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT11
Plasmid#223383PurposeT-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpRY-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT08
Plasmid#223380PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
Reporter-1
Plasmid#180218PurposeSurrogate reporter plasmid for the assay of targeted RNA editingDepositorInsertSurrogate reporter
UseLentiviralAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tg (mpx:mCherry-2A-Rac2D57N)
Plasmid#58934Purposeexpresses dominant negative rac2 tagged with mcherry in neutrophilsDepositorInsertmcherry-2a-Rac2D57N (rac2 Zebrafish)
Tagsmcherry2aMutationchanged Aspartate 57 to AsparaginePromotermpxAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pnos-Cas9-nos
Plasmid#62208PurposeExpresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.DepositorInsertcas9
ExpressionInsectPromoternanosAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_Ado1.0
Plasmid#153288PurposeExpress the adenosine sensor GRAB_Ado1.0 in astrocytesDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVPromoterGfaABC1DAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-cas9-2A-mCherry
Plasmid#107592PurposeAn entry vector with cas9 -2A-mCherryDepositorInsertcas9-2A-mCherry
UseCRISPRTags2A-mCherryAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHelper_AraC/pBad-Tnpase (camR)
Plasmid#213909PurposeArabinose-inducible himar1C9 transposase expression - cam resistanceDepositorInsertAraC/pBad ATc-inducible himar1C9 transposase
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-cMYC
Plasmid#216510PurposeExpresses MYC in human cells under a tetracycline/doxycycline-inducible promoterDepositorInsertcMYC
TagsHAExpressionMammalianAvailable SinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-NLS-BhCas12b_v4
Plasmid#158615PurposeFor protein expression of BhCas12b_v4DepositorInsertNLS-BhCas12b_v4
Tags6xHIS-TwinStrep-SUMOExpressionBacterialAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Reporter-2
Plasmid#180219PurposeSurrogate reporter plasmid for the assay of targeted RNA editingDepositorInsertSurrogate reporter
UseLentiviralAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORF1-2
Plasmid#206024PurposeAn expression vector for mouse LINE1 ORF1-2DepositorInsertORF1-2 derived from mouse LINE1
ExpressionMammalianAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LES2A-CRE
Plasmid#167933PurposeLentiviral CRE expression tracked by mScarlet-I fluorescence marker.DepositorInsertmScarlet-P2A-CRE
UseCre/Lox and LentiviralExpressionMammalianPromoterEF1aAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_Ado1.0mut
Plasmid#153287PurposeExpress the adenosine-insensitive control sensor GRAB_Ado1.0mut in neuronsDepositorInsertAdenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)
UseAAVPromoterhSynAvailable SinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT07
Plasmid#223379PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.DepositorInsertAtUBQ10-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-blast
Plasmid#210221PurposeIntegrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
Plasmid#41863PurposeThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.DepositorArticleInsertU6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
gRNA_GFP-T2
Plasmid#41820PurposeExpresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineeringDepositorInsertgRNA_GFP-T2
UseCRISPRExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHelper_TetR/pTet-Tnpase (camR)
Plasmid#213910PurposeATc-inducible himar1C9 transposase expression - cam resistanceDepositorInsertTetR/ptet ATc-inducible himar1C9 transposase
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK-Ma pylRS nitroY/haloY-F5
Plasmid#212125PurposeExpresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS 3-NY/HaloY F5
ExpressionBacterialMutationCTG125CTT, N166A V168S, A223C, W239RPromoterGlnS (constitutive)Available SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTK_Sox10_enh_10E2_Cherry
Plasmid#130590Purposeenhancer reporterDepositorInsertSox10_enh_10E2
UseEnhancer reporterPromoterthymidine kinase minimal promoterAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRGEB33
Plasmid#158151PurposeConstruction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.DepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AncMEcoGII291
Plasmid#205024PurposeExpressing engineered M.EcoGII with higher thermostability and DNA methylation activityDepositorInsertAncestral M.EcoGII 291
TagsHis6x tagExpressionBacterialPromoterT7Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTK_Pax7_enh_195_Citrine
Plasmid#130604Purposeenhancer reporterDepositorInsertPax7_enh_195
UseEnhancer reporterPromoterthymidine kinase minimal promoterAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZY101
Plasmid#73932PurposeThis binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.htmlDepositorInsertbar
ExpressionPlantPromoter35SAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-jGCaMP7b-XN
Plasmid#178362PurposeNuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.DepositorInsertjGCaMP7b-XN
ExpressionMammalianAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_9072_v1
Plasmid#126854Purpose9072 b scaffold from compatibility group 3, made by method IIDepositorInsertII_CS3_9072_v1
UseSynthetic BiologyAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only