432 results

#197934

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Kensaku Sakamoto

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

March 8, 2023

Academic Institutions and Nonprofits only

#49018

This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

George Church

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Feb. 18, 2014

Academic Institutions and Nonprofits only

#189857

Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

George Church

Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

Jan. 27, 2023

Academic Institutions and Nonprofits only

#182776

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted

Michael Jewett

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

April 18, 2023

Academic Institutions and Nonprofits only

#182778

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

Michael Jewett

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

April 18, 2023

Academic Institutions and Nonprofits only

#192872

MG1655-C321 -- All 321 UAG codons changed to UAA

Jesse Rinehart

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Dec. 14, 2022

Academic Institutions and Nonprofits only

#87359

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

George Church

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

May 1, 2017

Academic Institutions and Nonprofits only

#218774

E. coli strain, genotype: C321.ΔA pUltraBR-MjY ΔtyrS tyrTV::tolC tyrU::GentR lambda::ZeoR

Abhishek Chatterjee

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

June 28, 2024

Academic Institutions and Nonprofits only

#26242

Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

Opher Gileadi

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Sept. 6, 2019

Academic Institutions and Nonprofits only

#220921

cssDNA production strain

Shawn Douglas

Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

Aug. 19, 2024

Academic Institutions and Nonprofits only

#176583

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

Jean-Loup Faulon

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Dec. 14, 2021

Academic Institutions and Nonprofits only

#45472

Michele Swanson

Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

Feb. 24, 2016

Academic Institutions and Nonprofits only

#230034

Fluorescently labelled (mCherry) E. coli

Tobias Bergmiller, Calin Guet

Bergmiller et al Science. 2017 Apr 21;356(6335):311-315.

Feb. 3, 2025

Academic Institutions and Nonprofits only

#230033

Fluorescently labelled (monomeric sfGFP) E. coli

Tobias Bergmiller, Calin Guet

Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

Feb. 3, 2025

Academic Institutions and Nonprofits only

#195090

Improved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).

Sander Tans

Kogenaru et al J Biol Eng. 2014 Jan 2;8(1):2. doi: 10.1

Feb. 6, 2023

Academic Institutions and Nonprofits only

#45473

Michele Swanson

Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

Feb. 24, 2016

Academic Institutions and Nonprofits only

#196340

Bacterial strain lacking adenlyate cyclase (cyaA, needed for cAMP synthesis) and phosphodiesterase (cpdA, which degrades cAMP); unable to control its intracellular cAMP levels

Terence Hwa, Thomas Kuhlman

Kuhlman et al Proc Natl Acad Sci U S A. 2007 Apr 3;104

Nov. 12, 2025

Academic Institutions and Nonprofits only

#61440

This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

Kristala Prather

Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

Feb. 1, 2015

Academic Institutions and Nonprofits only

#37854

Kristala Prather

Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

Nov. 6, 2012

Academic Institutions and Nonprofits only

#248173

Maintenance of R6K plasmids at 13 PCN

Thomas Gorochowski

Hina-Nilesh Joshi et al ACS Synth Biol. 2026 Jan 13. doi: 10.102

March 9, 2026

Academic Institutions and Nonprofits only

#115925

Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

David Bikard

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

March 26, 2019

Academic Institutions and Nonprofits only

#197933

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Kensaku Sakamoto

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Nov. 27, 2023

Academic Institutions and Nonprofits only

#174513

Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

Jason W Chin

Fredens et al Nature. 2019 May;569(7757):514-518. doi:

Nov. 18, 2021

Academic Institutions and Nonprofits only

#197655

This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

Richard Cooley, Ryan Mehl

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

March 16, 2023

Academic Institutions and Nonprofits only

#237425

This engineered E. coli S17-1 λpir strain, featuring a mutation in the gyrA gene (462Arg→Cys), was designed to confer resistance to the CcdB toxin, allowing it to survive with a ccdB-carrying plasmid.

Xue Liu

Zhang et al Nat Commun. 2025 Jul 1;16(1):5870. doi:

Aug. 7, 2025

Academic Institutions and Nonprofits only

#124700

E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

Herbert Schweizer

Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

April 5, 2021

Academic Institutions and Nonprofits only

#98417

Bacterial Strain with Genotype: MC4100 ∆clpPX

Johan Paulsson

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

July 10, 2017

Academic Institutions and Nonprofits only

#248175

Maintenance of R6K plasmids at 95 PCN

Thomas Gorochowski

Hina-Nilesh Joshi et al ACS Synth Biol. 2026 Jan 13. doi: 10.102

March 9, 2026

Academic Institutions and Nonprofits only

#115924

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

David Bikard

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

March 26, 2019

Academic Institutions and Nonprofits only

#229395

Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

Michael Springer

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

March 24, 2025

Academic Institutions and Nonprofits only

#197132

Deficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.

Baojun Wang

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

July 11, 2023

Academic Institutions and Nonprofits only

#105064

host strain for phage assisted continuous evolution

David Liu

Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

March 23, 2018

Academic Institutions and Nonprofits only

#105063

host strain for phage assisted continuous evolution

David Liu

Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

March 21, 2018

Academic Institutions and Nonprofits only

#176581

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Chase Beisel, Jean-Loup Faulon

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Dec. 14, 2021

Academic Institutions and Nonprofits only

#248174

Maintenance of R6K plasmids at 59 PCN

Thomas Gorochowski

Hina-Nilesh Joshi et al ACS Synth Biol. 2026 Jan 13. doi: 10.102

March 9, 2026

Academic Institutions and Nonprofits only

#209018

Deficient in the P2 bacteriophage packaging cos site and encoding rhamnose-inducible P4 delta (δ) and epsilon (ε) genes

Alfonso Jaramillo

Tridgett et al ACS Synth Biol. 2021 Jan 15;10(1):107-11

Jan. 17, 2025

Academic Institutions and Nonprofits only

#99247

LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

Jeff Hasty

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Nov. 1, 2017

Academic Institutions and Nonprofits only

#52706

KIlac with lacZ deletion

Tanja Kortemme

Eames et al Science. 2012 May 18;336(6083):911-5. do

Feb. 2, 2015

Academic Institutions and Nonprofits only

#51950

broad-host range natural conjugative plasmid

Farren Isaacs

Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

May 1, 2014

Academic Institutions and Nonprofits only

#62077

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted.

Robert Gennis, Toshio Iwasaki

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Jan. 26, 2015

Academic Institutions and Nonprofits only