419 results

1. Syn61Δ3(ev5)

   Bacterial Strain

   #174514

   Purpose

   Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Bacterial Resistance

   Streptomycin

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

2. C321.∆A T7RNAP ompT- rne- lon-

   Bacterial Strain

   #182778

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Spectinomycin

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

3. B-95.ΔAΔfabR

   Bacterial Strain

   #197934

   Purpose

   BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

   Bacterial Resistance

   None

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

4. BL21ΔBCF

   Bacterial Strain

   #102269

   Purpose

   lamB, ompC, ompF triple knockout strain for overproduction of outer membrane proteins

   Depositor

   Jack Leo

   Article

   Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

   Bacterial Resistance

   None

   Available since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

5. C321.ΔA.exp

   Bacterial Strain

   #49018

   Purpose

   This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

   Depositor

   George Church

   Article

   Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

   Bacterial Resistance

   Bleocin (zeocin)

   Species

   Escherichia coli

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

6. EcNR2 Strain

   Bacterial Strain

   #26931

   Depositor

   George Church

   Article

   Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

   Bacterial Resistance

   Chloramphenicol and ampicillin

   Available since

   Jan. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

7. S2060

   Bacterial Strain

   #105064

   Purpose

   host strain for phage assisted continuous evolution

   Depositor

   David Liu

   Article

   Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

   Bacterial Resistance

   Streptomycin and tetracycline

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

8. BL21 ΔrecBCD

   Bacterial Strain

   #176581

   Purpose

   E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

   Depositor

   Jean-Loup Faulon, Chase Beisel

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Bacterial Resistance

   None

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

9. B-95.ΔA

   Bacterial Strain

   #197933

   Purpose

   BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

   Bacterial Resistance

   None

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

10. E. coli K-12 MG1655 RARE

    Bacterial Strain

    #61440

    Purpose

    This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

    Depositor

    Kristala Prather

    Article

    Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

    Bacterial Resistance

    None

    Available since

    Feb. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

11. BL21 Rosetta2 ΔrecBCD GamS

    Bacterial Strain

    #176586

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

12. BL21ΔCF

    Bacterial Strain

    #102265

    Purpose

    ompC, ompF double knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

13. BL21ΔF

    Bacterial Strain

    #102259

    Purpose

    ompF deletion strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

14. BL21ΔABC

    Bacterial Strain

    #102266

    Purpose

    ompA, lamB, ompC triple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

15. BL21(DE3) ΔserC

    Bacterial Strain

    #197656

    Purpose

    Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

    Depositor

    Richard Cooley, Ryan Mehl

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Bacterial Resistance

    None

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

16. DH10B-ALT

    Bacterial Strain

    #61151

    Purpose

    DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Bacterial Resistance

    None

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

17. MG1655 ΔendA ΔrecA (DE3)

    Bacterial Strain

    #37854

    Depositor

    Kristala Prather

    Article

    Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

    Bacterial Resistance

    None

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

18. Ochre (rEcΔ2.ΔA.B3.tW*)

    Bacterial Strain

    #234622

    Purpose

    Genomically recoded bacteria for site-specific incorporation of two nonstandard amino acids into proteins

    Depositor

    Farren Isaacs

    Article

    Grome et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Bacterial Resistance

    Hygromycin

    Available since

    May 16, 2025

    Availability

    Academic Institutions and Nonprofits only

19. B95(DE3) ΔA ΔfabR ΔserB

    Bacterial Strain

    #197655

    Purpose

    This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

    Depositor

    Richard Cooley, Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Bacterial Resistance

    None

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

20. JM107

    Bacterial Strain

    #49759

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Yanisch-Perron et al Gene. 1985;33(1):103-19.

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

21. JS200 strain

    Bacterial Strain

    #11794

    Depositor

    Lawrence Loeb

    Article

    Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

    Bacterial Resistance

    None

    Available since

    June 20, 2007

    Availability

    Academic Institutions and Nonprofits only

22. C321.ΔA

    Bacterial Strain

    #48998

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Ampicillin

    Species

    Escherichia coli

    Available since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

23. V. natriegens NC7

    Bacterial Strain

    #215356

    Purpose

    Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

    Depositor

    Buz Barstow

    Bacterial Resistance

    None

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

24. Corynebacterium glutamicum::RFP

    Bacterial Strain

    #79642

    Purpose

    Expresses RFP from the chromosome of Corynebacterium glutamicum ATCC 13032 (ptac promoter unrepressed; no LacI present)

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Bacterial Resistance

    Gentamicin

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

25. bMS.346

    Bacterial Strain

    #220588

    Purpose

    Increase editing efficiency of retron and oligo recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Bacterial Resistance

    None

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

26. MG1655 ΔendA ΔrecA

    Bacterial Strain

    #37853

    Depositor

    Kristala Prather

    Article

    Leonard et al Proc Natl Acad Sci U S A. 2010 Aug 3;107

    Bacterial Resistance

    None

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

27. RHO3

    Bacterial Strain

    #124700

    Purpose

    E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

    Depositor

    Herbert Schweizer

    Article

    Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

    Bacterial Resistance

    Dap (400 µg/ml)

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

28. AdEasier-1 cells (strain)

    Bacterial Strain

    #16399

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Bacterial Resistance

    Ampicillin and streptomycin

    Species

    Escherichia coli

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

29. DHL708

    Bacterial Strain

    #98417

    Purpose

    Bacterial Strain with Genotype: MC4100 ∆clpPX

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Bacterial Resistance

    Streptomycin

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

30. EC1000

    Bacterial Strain

    #71852

    Purpose

    RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

    Depositor

    Jan Kok, Todd Klaenhammer

    Article

    Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

    Bacterial Resistance

    Kanamycin

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

31. LC-E75

    Bacterial Strain

    #115925

    Purpose

    Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Bacterial Resistance

    None

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

32. FR-E01

    Bacterial Strain

    #118727

    Purpose

    Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

    Depositor

    David Bikard

    Article

    Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

    Bacterial Resistance

    None

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

33. BJ5183 cells

    Bacterial Strain

    #16398

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Bacterial Resistance

    Streptomycin

    Species

    Escherichia coli

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

34. BL21(DE3)-R3-pRARE2

    Bacterial Strain

    #26242

    Purpose

    Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Jun 10. ():.

    Bacterial Resistance

    Chloramphenicol

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

35. S1030

    Bacterial Strain

    #105063

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

    Bacterial Resistance

    Streptomycin and tetracycline

    Available since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

36. JM110

    Bacterial Strain

    #49763

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Yanisch-Perron et al Gene. 1985;33(1):103-19.

    Bacterial Resistance

    Streptomycin

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

37. ATMW-BL21

    Bacterial Strain

    #218769

    Purpose

    E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

    Bacterial Resistance

    Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

    Species

    Escherichia coli

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

38. MG1655-OptoCre-knt-P*-R

    Bacterial Strain

    #188476

    Purpose

    Cre-inducible activation of knt for kanamycin resistance.

    Depositor

    Mary Dunlop

    Article

    Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

    Bacterial Resistance

    None

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

39. ML14

    Bacterial Strain

    #61911

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

40. ∆cosσε ∆HG

    Bacterial Strain

    #197132

    Purpose

    Deficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Bacterial Resistance

    Hygromycin

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only