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rEcoli XpS

Bacterial Strain

#192872

Purpose

MG1655-C321 -- All 321 UAG codons changed to UAA

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
E. coli MEV20

Bacterial Strain

#197113

Purpose

E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
E. coli MEV15

Bacterial Strain

#197112

Purpose

E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
E. coli ER1821ΔlacI

Bacterial Strain

#141407

Purpose

LacI-deficient E. coli host bacterium that produces β-galactosidase constitutively

Depositor

Antal Kiss

Article

Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

Bacterial Resistance

None

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655 RARE

Bacterial Strain

#61440

Purpose

This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

Depositor

Kristala Prather

Article

Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

Bacterial Resistance

None

Available since

Feb. 1, 2015

Availability

Academic Institutions and Nonprofits only
E. coli BW25113 ΔtnaA ΔtrpR

Bacterial Strain

#205015

Purpose

Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

Depositor

Yajun Yan

Article

Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

Bacterial Resistance

None

Species

Escherichia coli

Available since

Aug. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits
Escherichia coli DH10B-MOB

Bacterial Strain

#229395

Purpose

Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Bacterial Resistance

60 ug/ml of dap

Species

Escherichia coli

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only
E. coli BL21 (DE3) ΔEntD

Bacterial Strain

#192874

Purpose

Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

Depositor

Christian Chalut, Christophe Guilhot

Article

Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP5(nth-ydgR)

Bacterial Strain

#110245

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP4(essQ-cspB)

Bacterial Strain

#110244

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP6(ygcE-ygcF)

Bacterial Strain

#110246

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP1(atpI-gidB)

Bacterial Strain

#110241

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the atpl-gidB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP3(ybbD-ylbG)

Bacterial Strain

#110243

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the ybbD-ylbG intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
CYN1 (E. coli Entner-Doudoroff v1)

Bacterial Strain

#71421

Purpose

This E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway (first version). The module also contains a Cm resistance marker.

Depositor

Howard Salis

Article

Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

Bacterial Resistance

Chloramphenicol

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP2(yieN-trkD)

Bacterial Strain

#110242

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the yieN-trkD intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli MG1655-Landing Pad-Clone 3

Bacterial Strain

#200007

Purpose

Landing pad (with pIP1433 seed promoter) in MG1655 clone 3

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Bacterial Resistance

Apramycin

Species

Synthetic

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only
DH10B E. coli aptI/gidB::Landing Pad

Bacterial Strain

#83036

Purpose

For site-specific chromosomal insertion of a target DNA sequence at an engineered landing pad site in the atpI/gidB locus.

Depositor

Cammie Lesser

Article

Reeves et al ACS Synth Biol. 2015 May 15;4(5):644-54.

Bacterial Resistance

Tetracycline

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only
CYN2/150 (E. coli Entner-Doudoroff v11)

Bacterial Strain

#71420

Purpose

This E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway with an improved NADPH regeneration rate. Module is Cm resistant

Depositor

Howard Salis

Article

Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

Bacterial Resistance

Chloramphenicol

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
ATMY3

Bacterial Strain

#218771

Purpose

E. coli strain, genotype: EcNR1GT pUltraBR MjY dtyrS tyrTV::CUA tyrU::inact lambda::tolC

Depositor

Abhishek Chatterjee

Article

Italia et al Cell Chem Biol. 2018 Oct 18;25(10):1304-

Bacterial Resistance

Spectinomycin

Species

Escherichia coli

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
ATMY4

Bacterial Strain

#218772

Purpose

E. coli strain, genotype: EcNR1GT pUltraBR MjY dtyrS tyrTV::CUA tyrU::CUA lambda::tolC

Depositor

Abhishek Chatterjee

Article

Italia et al Cell Chem Biol. 2018 Oct 18;25(10):1304-

Bacterial Resistance

Spectinomycin

Species

Escherichia coli

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
ATMY5

Bacterial Strain

#218773

Purpose

E. coli strain, genotype: EcNR1GT pUltraBR MjY dtyrS tyrTV::inact tyrU::inact lambda::tolC

Depositor

Abhishek Chatterjee

Article

Italia et al Cell Chem Biol. 2018 Oct 18;25(10):1304-

Bacterial Resistance

Spectinomycin

Species

Escherichia coli

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
ATMY-C321

Bacterial Strain

#218774

Purpose

E. coli strain, genotype: C321.ΔA pUltraBR-MjY ΔtyrS tyrTV::tolC tyrU::GentR lambda::ZeoR

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Bacterial Resistance

Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

Species

Escherichia coli

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only
ATMY1

Bacterial Strain

#218770

Purpose

E. coli strain, genotype: EcNR1GT pUltraBR MjY dtyrS dtyrTV::tolC tyrU::gentR lambda::ZeoR

Depositor

Abhishek Chatterjee

Article

Italia et al Cell Chem Biol. 2018 Oct 18;25(10):1304-

Bacterial Resistance

Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

Species

Escherichia coli

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
ATMW-BL21

Bacterial Strain

#218769

Purpose

E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

Depositor

Abhishek Chatterjee

Article

Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

Bacterial Resistance

Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

Species

Escherichia coli

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
BM28

Bacterial Strain

#201047

Purpose

MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures. Also carries a pBR325 plasmid carrying groESL called pOF39.

Depositor

Jeannette Winter

Article

McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi

Bacterial Resistance

Ampicillin and kanamycin

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only
BA14

Bacterial Strain

#186997

Purpose

Deletion of the nuo operon

Depositor

Steven Vik

Article

Amarneh et al Biochim Biophys Acta. 2006 Dec;1757(12):

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only
C321.ΔA

Bacterial Strain

#48998

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Ampicillin

Species

Escherichia coli

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
C321

Bacterial Strain

#48999

Purpose

Recoded E. coli MG1655 strain with wt UAG termination function (RF1 is still present)

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Ampicillin

Species

Escherichia coli

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
AV04

Bacterial Strain

#115926

Purpose

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Bacterial Resistance

None

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only
LC-E02

Bacterial Strain

#78551

Purpose

E. coli strain K-12 MG1655, pTet--dCas9 cassette integrated at 186 primary attB site.

Depositor

David Bikard

Article

Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

Bacterial Resistance

None

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only
LC-E03

Bacterial Strain

#78552

Purpose

E. coli strain K-12 MG1655, pTet--Cas9 cassette integrated at 186 primary attB site.

Depositor

David Bikard

Article

Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

Bacterial Resistance

None

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only
FR-E01

Bacterial Strain

#118727

Purpose

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

Bacterial Resistance

None

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only
LC-E18

Bacterial Strain

#115924

Purpose

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Bacterial Resistance

None

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only
S4197

Bacterial Strain

#200839

Purpose

wildtype strain MG1655 rph+, ilvG+, ΔlacZ

Depositor

Karin Schnetz

Article

Venkatesh et al J Bacteriol. 2010 Dec;192(24):6456-64. d

Bacterial Resistance

None

Species

Escherichia coli str. k-12 substr. mg1655

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only
SJ358

Bacterial Strain

#67755

Purpose

a derivative of E. coli strain NCM3722 which has been cured of the F plasmid

Depositor

Suckjoon Jun

Article

Brown et al Genome Announc. 2015 Aug 6;3(4). pii: e0

Bacterial Resistance

None

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
MCJ.559

Bacterial Strain

#69493

Purpose

Strain with recoded TAG and genomic deletions for improved incorporation

Depositor

Michael Jewett

Article

Hong et al Chembiochem. 2015 Mar 23;16(5):844-53. d

Bacterial Resistance

Ampicillin

Species

Eschericia coli

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
MS1

Bacterial Strain

#61960

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE hisG genes deleted

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
LC-E75

Bacterial Strain

#115925

Purpose

Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Bacterial Resistance

None

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only
eScaf

Bacterial Strain

#220921

Purpose

cssDNA production strain

Depositor

Shawn Douglas

Article

Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

Bacterial Resistance

None

Species

Escherichia coli

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only
BM28 ΔlysU

Bacterial Strain

#201048

Purpose

MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.

Depositor

Jeannette Winter

Article

McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi

Bacterial Resistance

Kanamycin

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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