422 results

1. MG1655 ΔendA ΔrecA (DE3)

   Bacterial Strain

   #37854

   Depositor

   Kristala Prather

   Article

   Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

   Bacterial Resistance

   None

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

2. C321.∆A T7RNAP ompT- rne-

   Bacterial Strain

   #182777

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Spectinomycin

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

3. EC1000

   Bacterial Strain

   #71852

   Purpose

   RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

   Depositor

   Jan Kok, Todd Klaenhammer

   Article

   Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

   Bacterial Resistance

   Kanamycin

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

4. V. natriegens NC1

   Bacterial Strain

   #215355

   Purpose

   Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

   Depositor

   Buz Barstow

   Bacterial Resistance

   None

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

5. DH5alpha::DddI

   Bacterial Strain

   #179631

   Purpose

   For expression of toxic DddA

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Bacterial Resistance

   Gentamicin

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

6. LC-E75

   Bacterial Strain

   #115925

   Purpose

   Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Bacterial Resistance

   None

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

7. ATMW-BL21

   Bacterial Strain

   #218769

   Purpose

   E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

   Depositor

   Abhishek Chatterjee

   Article

   Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

   Bacterial Resistance

   Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

   Species

   Escherichia coli

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

8. MG1655 ΔendA ΔrecA

   Bacterial Strain

   #37853

   Depositor

   Kristala Prather

   Article

   Leonard et al Proc Natl Acad Sci U S A. 2010 Aug 3;107

   Bacterial Resistance

   None

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

9. sAJM.1504

   Bacterial Strain

   #108251

   Purpose

   Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Bacterial Resistance

   Chloramphenicol

   Available since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

10. EcNR2 Strain

    Bacterial Strain

    #26931

    Depositor

    George Church

    Article

    Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

11. nuc5-

    Bacterial Strain

    #40804

    Depositor

    George Church

    Article

    Mosberg et al PLoS One. 2012;7(9):e44638. Epub 2012 Se

    Bacterial Resistance

    Ampicillin

    Available since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. Syn61Δ3(ev5) ΔrecA (ev1)

    Bacterial Strain

    #189857

    Purpose

    Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

    Depositor

    George Church

    Article

    Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

    Bacterial Resistance

    Streptomycin

    Species

    Synthetic

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

13. E. coli MEV20

    Bacterial Strain

    #197113

    Purpose

    E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Bacterial Resistance

    None

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

14. E. coli BW25113 ΔtnaA ΔtrpR

    Bacterial Strain

    #205015

    Purpose

    Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

    Depositor

    Yajun Yan

    Article

    Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Aug. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

15. JM83

    Bacterial Strain

    #50348

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Messing et al Recombinant DNA Technical Bulletin, 1979

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

16. BL21ΔABCF

    Bacterial Strain

    #102270

    Purpose

    ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    Tetracycline

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

17. TB204 △metA

    Bacterial Strain

    #230039

    Purpose

    Fluorescently labelled (GFP) E. coli, auxotrophic for methionine

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

18. pRK24

    Bacterial Strain

    #51950

    Purpose

    broad-host range natural conjugative plasmid

    Depositor

    Farren Isaacs

    Article

    Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

    Bacterial Resistance

    Tetracycline

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

19. rEcoli XpS

    Bacterial Strain

    #192872

    Purpose

    MG1655-C321 -- All 321 UAG codons changed to UAA

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

    Bacterial Resistance

    Bleocin (zeocin)

    Species

    Escherichia coli

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

20. MG1655GFP

    Bacterial Strain

    #109393

    Purpose

    MG1655 with a "capacity monitor" inserted in the lambda phage integration site.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Bacterial Resistance

    Kanamycin

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

21. JM109

    Bacterial Strain

    #49761

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Yanisch-Perron et al Gene. 1985;33(1):103-19.

    Bacterial Resistance

    None

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

22. C321.∆A.opt

    Bacterial Strain

    #87359

    Purpose

    This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

    Depositor

    George Church

    Article

    Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

    Bacterial Resistance

    Gentamicin

    Species

    Synthetic

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

23. DHIP1

    Bacterial Strain

    #226851

    Purpose

    Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

    Depositor

    David Nielsen

    Article

    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

    Bacterial Resistance

    Streptomycin

    Species

    Escherichia coli

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

24. RF13

    Bacterial Strain

    #62078

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

25. CJW4617

    Bacterial Strain

    #177118

    Purpose

    E. coli strain carrying IPTG-inducible gfp-muNS gene for monitoring the mobility of cytoplasmic particles.

    Depositor

    Christine Jacobs-Wagner

    Article

    Parry et al Cell. 2014 Jan 16;156(1-2):183-94. doi:

    Bacterial Resistance

    Kanamycin

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

26. BL21ΔBCF

    Bacterial Strain

    #102269

    Purpose

    lamB, ompC, ompF triple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

27. 39R861+

    Bacterial Strain

    #119737

    Purpose

    Six stable plasmids can be used as size standards

    Depositor

    Ruth Hall

    Article

    Moran et al Plasmid. 2019 Mar;102:6-9. doi: 10.1016/

    Bacterial Resistance

    None

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

28. ∆cosσε ∆HG

    Bacterial Strain

    #197132

    Purpose

    Deficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Bacterial Resistance

    Hygromycin

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

29. BL21 Rosetta2 ΔrecBCD GamS

    Bacterial Strain

    #176586

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

30. DH10B-ALT

    Bacterial Strain

    #61151

    Purpose

    DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Bacterial Resistance

    None

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

31. Strain SIJ_488

    Bacterial Strain

    #68246

    Purpose

    E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Bacterial Resistance

    None

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

32. C321.ΔA.M9adapted

    Bacterial Strain

    #98568

    Purpose

    Recoded strain C321.ΔA adaptively evolved for 1000+ generations in M9 minimal media + 1% glucose and biotin

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

    Bacterial Resistance

    Ampicillin

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

33. BL21ΔCF

    Bacterial Strain

    #102265

    Purpose

    ompC, ompF double knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

34. sAJM.1506

    Bacterial Strain

    #108254

    Purpose

    Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Bacterial Resistance

    Chloramphenicol

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

35. EcNR1 Strain

    Bacterial Strain

    #26930

    Depositor

    George Church

    Article

    Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

    Bacterial Resistance

    Ampicillin

    Available since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

36. SQ171/pCSacB/ptRNA100

    Bacterial Strain

    #155206

    Purpose

    SQ171 strain supported by pCSacB and ptRNA100 plasmids

    Depositor

    Alexander Mankin

    Article

    Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

    Bacterial Resistance

    Kanamycin

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

37. C321.deltaA (Isaacs lab)

    Bacterial Strain

    #73581

    Purpose

    Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Bacterial Resistance

    Ampicillin

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

38. BL21 Rosetta2 ΔrecBCD

    Bacterial Strain

    #176583

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

39. DHL708

    Bacterial Strain

    #98417

    Purpose

    Bacterial Strain with Genotype: MC4100 ∆clpPX

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Bacterial Resistance

    Streptomycin

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

40. ML14

    Bacterial Strain

    #61911

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only