419 results

1. LC-E18

   Bacterial Strain

   #115924

   Purpose

   Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Bacterial Resistance

   None

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

2. SQ171/pCSacB/ptRNA100

   Bacterial Strain

   #155206

   Purpose

   SQ171 strain supported by pCSacB and ptRNA100 plasmids

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Bacterial Resistance

   Kanamycin

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

3. BL21ΔABCF

   Bacterial Strain

   #102270

   Purpose

   ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

   Depositor

   Jack Leo

   Article

   Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

   Bacterial Resistance

   Tetracycline

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

4. rEcoli XpS

   Bacterial Strain

   #192872

   Purpose

   MG1655-C321 -- All 321 UAG codons changed to UAA

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

   Bacterial Resistance

   Bleocin (zeocin)

   Species

   Escherichia coli

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

5. CJW4617

   Bacterial Strain

   #177118

   Purpose

   E. coli strain carrying IPTG-inducible gfp-muNS gene for monitoring the mobility of cytoplasmic particles.

   Depositor

   Christine Jacobs-Wagner

   Article

   Parry et al Cell. 2014 Jan 16;156(1-2):183-94. doi:

   Bacterial Resistance

   Kanamycin

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

6. BL21∆serB

   Bacterial Strain

   #34929

   Depositor

   Dieter Söll, Jesse Rinehart

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Bacterial Resistance

   None

   Available since

   Feb. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

7. C321.∆A.opt

   Bacterial Strain

   #87359

   Purpose

   This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

   Depositor

   George Church

   Article

   Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

   Bacterial Resistance

   Gentamicin

   Species

   Synthetic

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

8. eScaf

   Bacterial Strain

   #220921

   Purpose

   cssDNA production strain

   Depositor

   Shawn Douglas

   Article

   Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

9. Syn61Δ3(ev5) ΔrecA (ev1)

   Bacterial Strain

   #189857

   Purpose

   Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

   Depositor

   George Church

   Article

   Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

   Bacterial Resistance

   Streptomycin

   Species

   Synthetic

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

10. pRK24

    Bacterial Strain

    #51950

    Purpose

    broad-host range natural conjugative plasmid

    Depositor

    Farren Isaacs

    Article

    Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

    Bacterial Resistance

    Tetracycline

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

11. C321.ΔA.M9adapted

    Bacterial Strain

    #98568

    Purpose

    Recoded strain C321.ΔA adaptively evolved for 1000+ generations in M9 minimal media + 1% glucose and biotin

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

    Bacterial Resistance

    Ampicillin

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

12. sAJM.1504

    Bacterial Strain

    #108251

    Purpose

    Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Bacterial Resistance

    Chloramphenicol

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

13. C321.deltaA (Isaacs lab)

    Bacterial Strain

    #73581

    Purpose

    Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Bacterial Resistance

    Ampicillin

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

14. RF11

    Bacterial Strain

    #61961

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

15. TB205

    Bacterial Strain

    #230034

    Purpose

    Fluorescently labelled (mCherry) E. coli

    Depositor

    Calin Guet, Tobias Bergmiller

    Article

    Bergmiller et al Science. 2017 Apr 21;356(6335):311-315.

    Bacterial Resistance

    None

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

16. BL21 Rosetta2 ΔrecBCD

    Bacterial Strain

    #176583

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

17. HE_150

    Bacterial Strain

    #201051

    Purpose

    DH10B-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.

    Depositor

    Francis Nano

    Bacterial Resistance

    Streptomycin

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

18. sAJM.1506

    Bacterial Strain

    #108254

    Purpose

    Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Bacterial Resistance

    Chloramphenicol

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

19. Strain SIJ_488

    Bacterial Strain

    #68246

    Purpose

    E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Bacterial Resistance

    None

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. MK01

    Bacterial Strain

    #195090

    Purpose

    Improved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).

    Depositor

    Sander Tans

    Article

    Kogenaru et al J Biol Eng. 2014 Jan 2;8(1):2. doi: 10.1

    Bacterial Resistance

    Chloramphenicol

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

21. V. natriegens NC1

    Bacterial Strain

    #215355

    Purpose

    Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

    Depositor

    Buz Barstow

    Bacterial Resistance

    None

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

22. FW102 OL2-62

    Bacterial Strain

    #53735

    Purpose

    Bacterial-two hybrid reporter strain

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Bacterial Resistance

    Kanamycin and streptomycin

    Species

    Escherichia coli

    Available since

    June 18, 2014

    Availability

    Academic Institutions and Nonprofits only

23. CLM24 ∆lpxM

    Bacterial Strain

    #132780

    Purpose

    CLM24 strain with lpxM deletion

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Bacterial Resistance

    None

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

24. E. coli BW25113 ΔtnaA ΔtrpR

    Bacterial Strain

    #205015

    Purpose

    Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

    Depositor

    Yajun Yan

    Article

    Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Aug. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

25. Strain: C321.ΔA

    Bacterial Strain

    #68306

    Purpose

    Genomically recoded strain of E. coli (no genomic TAG)

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Bacterial Resistance

    Bleocin (zeocin)

    Available since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

26. Jun lab tunable CRISPRi strain

    Bacterial Strain

    #86400

    Purpose

    Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

    Depositor

    Suckjoon Jun

    Article

    Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

    Bacterial Resistance

    Spectinomycin and hygromycin

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

27. MM39

    Bacterial Strain

    #42894

    Depositor

    Bryan Berger

    Article

    Su et al J Biol Chem. 2012 Jul 22.

    Bacterial Resistance

    Streptomycin

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

28. LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

    Bacterial Strain

    #99247

    Purpose

    LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

    Depositor

    Jeff Hasty

    Article

    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

    Bacterial Resistance

    Tetracycline

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

29. DHIP1

    Bacterial Strain

    #226851

    Purpose

    Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

    Depositor

    David Nielsen

    Article

    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

    Bacterial Resistance

    Streptomycin

    Species

    Escherichia coli

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

30. sAJM.1505

    Bacterial Strain

    #108253

    Purpose

    Marionette-Pro. Marionette cluster inserted in the direction of leading strand replication, between 3,720,027 and 3,721,644 in E. coli BL21

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Bacterial Resistance

    Chloramphenicol

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

31. Escherichia coli DH10B-MOB

    Bacterial Strain

    #229395

    Purpose

    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Bacterial Resistance

    60 ug/ml of dap

    Species

    Escherichia coli

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

32. MG1655-OptoCre-knt-P**-R

    Bacterial Strain

    #188477

    Purpose

    Cre-inducible activation of knt for kanamycin resistance.

    Depositor

    Mary Dunlop

    Article

    Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

    Bacterial Resistance

    None

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

33. JM109

    Bacterial Strain

    #49761

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Yanisch-Perron et al Gene. 1985;33(1):103-19.

    Bacterial Resistance

    None

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

34. TB204

    Bacterial Strain

    #230033

    Purpose

    Fluorescently labelled (monomeric sfGFP) E. coli

    Depositor

    Calin Guet, Tobias Bergmiller

    Article

    Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

    Bacterial Resistance

    None

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

35. C-5545 ∆cosδε

    Bacterial Strain

    #209018

    Purpose

    Deficient in the P2 bacteriophage packaging cos site and encoding rhamnose-inducible P4 delta (δ) and epsilon (ε) genes

    Depositor

    Alfonso Jaramillo

    Article

    Tridgett et al ACS Synth Biol. 2021 Jan 15;10(1):107-11

    Bacterial Resistance

    Hygromycin

    Species

    Escherichia coli

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

36. TB201

    Bacterial Strain

    #230031

    Purpose

    Fluorescently labelled (YFP) E. coli

    Depositor

    Calin Guet, Tobias Bergmiller

    Article

    Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

    Bacterial Resistance

    None

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

37. TB205 △ilvA

    Bacterial Strain

    #230042

    Purpose

    Fluorescently labelled (mCherry) E. coli, auxotrophic for isoleucine

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

38. TB204 △ilvA

    Bacterial Strain

    #230041

    Purpose

    Fluorescently labelled (GFP) E. coli, auxotrophic for isoleucine

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

39. TB205 △metA

    Bacterial Strain

    #230040

    Purpose

    Fluorescently labelled (mCherry) E. coli, auxotrophic for methionine

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

40. TB204 △metA

    Bacterial Strain

    #230039

    Purpose

    Fluorescently labelled (GFP) E. coli, auxotrophic for methionine

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only