425 results

#230031

Fluorescently labelled (YFP) E. coli

Tobias Bergmiller, Calin Guet

Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

Feb. 3, 2025

Academic Institutions and Nonprofits only

#115925

Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

David Bikard

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

March 26, 2019

Academic Institutions and Nonprofits only

#197112

E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Christopher Voigt

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

June 30, 2023

Academic Institutions and Nonprofits only

#230032

Fluorescently labelled (mCerulean) E. coli

Tobias Bergmiller, Calin Guet

Feb. 3, 2025

Academic Institutions and Nonprofits only

#156460

An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.

Matthew D. Shoulders

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Oct. 19, 2020

Academic Institutions and Nonprofits only

#105063

host strain for phage assisted continuous evolution

David Liu

Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

March 21, 2018

Academic Institutions and Nonprofits only

#192874

Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

Christian Chalut, Christophe Guilhot

Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

Sept. 7, 2023

Academic Institutions and Nonprofits only

#226851

Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

David Nielsen

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Nov. 15, 2024

Academic Institutions and Nonprofits only

#87359

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

George Church

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

May 1, 2017

Academic Institutions and Nonprofits only

#176582

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

Jean-Loup Faulon

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Dec. 14, 2021

Academic Institutions and Nonprofits only

#37853

Kristala Prather

Leonard et al Proc Natl Acad Sci U S A. 2010 Aug 3;107

Nov. 6, 2012

Academic Institutions and Nonprofits only

#108254

Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

Christopher Voigt

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

June 26, 2018

Academic Institutions and Nonprofits only

#192872

MG1655-C321 -- All 321 UAG codons changed to UAA

Jesse Rinehart

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Dec. 14, 2022

Academic Institutions and Nonprofits only

#141407

LacI-deficient E. coli host bacterium that produces β-galactosidase constitutively

Antal Kiss

Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

Aug. 4, 2020

Academic Institutions and Nonprofits only

#182775

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

Michael Jewett

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

April 18, 2023

Academic Institutions and Nonprofits only

#218769

E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

Abhishek Chatterjee

Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

June 21, 2024

Academic Institutions and Nonprofits only

#73581

Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

Farren Isaacs

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

March 4, 2016

Academic Institutions and Nonprofits only

#71852

RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

Todd Klaenhammer, Jan Kok

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Feb. 22, 2016

Academic Institutions and Nonprofits only

#189857

Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

George Church

Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

Jan. 27, 2023

Academic Institutions and Nonprofits only

#65915

E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

Keith Tyo

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

March 1, 2016

Academic Institutions and Nonprofits only

#61151

DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

Matthew Bennett

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Oct. 2, 2015

Academic Institutions and Nonprofits only

#98417

Bacterial Strain with Genotype: MC4100 ∆clpPX

Johan Paulsson

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

July 10, 2017

Academic Institutions and Nonprofits only

#174513

Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

Jason W Chin

Fredens et al Nature. 2019 May;569(7757):514-518. doi:

Nov. 18, 2021

Academic Institutions and Nonprofits only

#218774

E. coli strain, genotype: C321.ΔA pUltraBR-MjY ΔtyrS tyrTV::tolC tyrU::GentR lambda::ZeoR

Abhishek Chatterjee

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

June 28, 2024

Academic Institutions and Nonprofits only

#191530

Contains a knockout gene in the E. coli strain BL21-AI

Seth Shipman

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Nov. 2, 2022

Academic Institutions and Nonprofits only

#26931

George Church

Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

Jan. 28, 2011

Academic Institutions and Nonprofits only

#115924

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

David Bikard

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

March 26, 2019

Academic Institutions and Nonprofits only

#45472

Michele Swanson

Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

Feb. 24, 2016

Academic Institutions and Nonprofits only

#68306

Genomically recoded strain of E. coli (no genomic TAG)

Jesse Rinehart

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Sept. 29, 2015

Academic Institutions and Nonprofits only

#220588

Increase editing efficiency of retron and oligo recombineering

Seth Shipman

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

May 16, 2024

Academic Institutions and Nonprofits only

#49763

E.coli K-12 derivative

Joachim Messing

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Oct. 14, 2014

Academic Institutions and Nonprofits only

#110245

E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Sriram Kosuri

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

March 5, 2019

Academic Institutions and Nonprofits only

#18049

Scot Wolfe

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

July 11, 2008

Academic Institutions and Nonprofits only

#215355

Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

Buz Barstow

Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

Feb. 22, 2024

Academic Institutions and Nonprofits only

#115926

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

David Bikard

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

March 26, 2019

Academic Institutions and Nonprofits only

#68246

E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.

Alex Nielsen

Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

Dec. 8, 2015

Academic Institutions and Nonprofits only

#99247

LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

Jeff Hasty

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Nov. 1, 2017

Academic Institutions and Nonprofits only

#50348

E.coli K-12 derivative

Joachim Messing

Messing et al Recombinant DNA Technical Bulletin, 1979

Oct. 14, 2014

Academic Institutions and Nonprofits only

#61911

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted.

Robert Gennis, Toshio Iwasaki

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Jan. 26, 2015

Academic Institutions and Nonprofits only

#49761

E.coli K-12 derivative

Joachim Messing

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Sept. 29, 2014

Academic Institutions and Nonprofits only