422 results

1. RF15

   Bacterial Strain

   #102799

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted.

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

   Bacterial Resistance

   None

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

2. ML8

   Bacterial Strain

   #61909

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. argH gene deleted.

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   Kanamycin

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

3. BLIM cells

   Bacterial Strain

   #35609

   Depositor

   Kathleen Matthews

   Article

   Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

   Bacterial Resistance

   None

   Available since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

4. ML3

   Bacterial Strain

   #61907

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG gene deleted.

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   Kanamycin

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

5. LC-E02

   Bacterial Strain

   #78551

   Purpose

   E. coli strain K-12 MG1655, pTet--dCas9 cassette integrated at 186 primary attB site.

   Depositor

   David Bikard

   Article

   Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

   Bacterial Resistance

   None

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

6. MG1655 Z1 malE

   Bacterial Strain

   #65915

   Purpose

   E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

   Depositor

   Keith Tyo

   Article

   Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

   Bacterial Resistance

   None

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

7. SKA974

   Bacterial Strain

   #80403

   Purpose

   JT2-based E. coli host strain for optogenetic control of cell growth.

   Depositor

   Mustafa Khammash

   Article

   Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

   Bacterial Resistance

   Kanamycin

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

8. JM106

   Bacterial Strain

   #49757

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Yanisch-Perron et al Gene. 1985;33(1):103-19.

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

9. RF1

   Bacterial Strain

   #61962

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. glyA gene deleted

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

10. RF5

    Bacterial Strain

    #62073

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB hisG genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

11. ML2

    Bacterial Strain

    #61906

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. ilvE gene deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

    Bacterial Resistance

    Kanamycin

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

12. RF10

    Bacterial Strain

    #62076

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. lysA genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

13. delta-ZYA

    Bacterial Strain

    #52695

    Purpose

    MG1655 with Lac Operon removed from native locus

    Depositor

    Tanja Kortemme

    Article

    Eames et al Science. 2012 May 18;336(6083):911-5. do

    Bacterial Resistance

    None

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

14. S4197

    Bacterial Strain

    #200839

    Purpose

    wildtype strain MG1655 rph+, ilvG+, ΔlacZ

    Depositor

    Karin Schnetz

    Article

    Venkatesh et al J Bacteriol. 2010 Dec;192(24):6456-64. d

    Bacterial Resistance

    None

    Species

    Escherichia coli str. k-12 substr. mg1655

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

15. LC-E03

    Bacterial Strain

    #78552

    Purpose

    E. coli strain K-12 MG1655, pTet--Cas9 cassette integrated at 186 primary attB site.

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Bacterial Resistance

    None

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

16. C321.∆A ompT- rne- endA- lon-

    Bacterial Strain

    #182775

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Ampicillin

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

17. E. coli MG1655-Landing Pad-Clone 3

    Bacterial Strain

    #200007

    Purpose

    Landing pad (with pIP1433 seed promoter) in MG1655 clone 3

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Bacterial Resistance

    Apramycin

    Species

    Synthetic

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

18. BL21 ΔrecB

    Bacterial Strain

    #176580

    Purpose

    E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression.

    Depositor

    Jean-Loup Faulon, Chase Beisel

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    None

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

19. ML6

    Bacterial Strain

    #61908

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. ilvE, avtA genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

    Bacterial Resistance

    Kanamycin

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

20. C321.∆A ompT- rne- endA-

    Bacterial Strain

    #182774

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Ampicillin

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

21. RF17

    Bacterial Strain

    #102801

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

    Bacterial Resistance

    None

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

22. delta-Z

    Bacterial Strain

    #52706

    Purpose

    KIlac with lacZ deletion

    Depositor

    Tanja Kortemme

    Article

    Eames et al Science. 2012 May 18;336(6083):911-5. do

    Bacterial Resistance

    None

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

23. E. coli K-12 MG1655_rLP5(nth-ydgR)

    Bacterial Strain

    #110245

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

24. EcAR7

    Bacterial Strain

    #52055

    Purpose

    Strain for use in Rinehart Lab Phosphoprotein synthesis kit

    Depositor

    Jesse Rinehart

    Article

    Heinemann et al FEBS Lett. 2012 Oct 19;586(20):3716-22.

    Bacterial Resistance

    None

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

25. HL 770

    Bacterial Strain

    #60369

    Purpose

    Lim Lab Strain

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Bacterial Resistance

    None

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

26. HL 1285

    Bacterial Strain

    #52942

    Purpose

    MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

27. BL21ΔAC

    Bacterial Strain

    #102261

    Purpose

    ompA, ompC double knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

28. Strain KJ1C

    Bacterial Strain

    #21874

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Protoc. 2009. 4(10):1471-501.

    Bacterial Resistance

    Tetracycline

    Available since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

29. HL 1128

    Bacterial Strain

    #60372

    Purpose

    Lim Lab Strain

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Bacterial Resistance

    None

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

30. ML17

    Bacterial Strain

    #61912

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. glnA gene deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

31. CY008

    Bacterial Strain

    #72401

    Purpose

    Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA

    Depositor

    Matthew Bennett

    Article

    Chen et al Science. 2015 Aug 28;349(6251):986-9. do

    Bacterial Resistance

    None

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

32. BL21ΔC

    Bacterial Strain

    #102258

    Purpose

    ompC deletion strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

33. Strain CSH100

    Bacterial Strain

    #21875

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Protoc. 2009. 4(10):1471-501.

    Bacterial Resistance

    None

    Available since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

34. KIlac

    Bacterial Strain

    #52696

    Purpose

    MG1655 with Lac Operon removed from native locus and re-integrated at the attTn7 locus

    Depositor

    Tanja Kortemme

    Article

    Eames et al Science. 2012 May 18;336(6083):911-5. do

    Bacterial Resistance

    None

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

35. SJ358

    Bacterial Strain

    #67755

    Purpose

    a derivative of E. coli strain NCM3722 which has been cured of the F plasmid

    Depositor

    Suckjoon Jun

    Article

    Brown et al Genome Announc. 2015 Aug 6;3(4). pii: e0

    Bacterial Resistance

    None

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

36. E. coli ER1821ΔlacI

    Bacterial Strain

    #141407

    Purpose

    LacI-deficient E. coli host bacterium that produces β-galactosidase constitutively

    Depositor

    Antal Kiss

    Article

    Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

    Bacterial Resistance

    None

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

37. Akaby

    Bacterial Strain

    #184224

    Purpose

    Akaby is a RecB knockout E. coli strain, made for cell-free expression system for linear templates.

    Depositor

    Kate Adamala

    Article

    Sato et al PLoS One. 2022 Apr 7;17(4):e0266272. doi

    Bacterial Resistance

    Kanamycin

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

38. CYN2/150 (E. coli Entner-Doudoroff v11)

    Bacterial Strain

    #71420

    Purpose

    This E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway with an improved NADPH regeneration rate. Module is Cm resistant

    Depositor

    Howard Salis

    Article

    Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

    Bacterial Resistance

    Chloramphenicol

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

39. DH10B E. coli aptI/gidB::Landing Pad

    Bacterial Strain

    #83036

    Purpose

    For site-specific chromosomal insertion of a target DNA sequence at an engineered landing pad site in the atpI/gidB locus.

    Depositor

    Cammie Lesser

    Article

    Reeves et al ACS Synth Biol. 2015 May 15;4(5):644-54.

    Bacterial Resistance

    Tetracycline

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

40. EcM2.1.dtolC

    Bacterial Strain

    #64053

    Purpose

    Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

    Depositor

    George Church

    Article

    Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

    Bacterial Resistance

    Ampicillin

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only