432 results

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RF1

Bacterial Strain

#61962

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. glyA gene deleted

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF11

Bacterial Strain

#61961

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
BL21ΔF

Bacterial Strain

#102259

Purpose

ompF deletion strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

None

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecBCD GamS

Bacterial Strain

#176586

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and Ampicillin

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
SB39

Bacterial Strain

#235121

Purpose

E. coli strain suitable for cloning, expression, library construction (good electroporation yield) and quantitative bacterial two-hybrid (qB2H).

Depositor

Oscar Ramos

Bacterial Resistance

None

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only
IF189

Bacterial Strain

#134836

Purpose

Rapid and efficient recombineering and purificiation of modified lambda phage DNA

Depositor

IIya Finkelstein

Article

Kim et al Sci Rep. 2017 May 18;7(1):2071. doi: 10.

Bacterial Resistance

None

Available Since

Jan. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits
AV04

Bacterial Strain

#115926

Purpose

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Bacterial Resistance

None

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only
E. coli ER1821ΔlacI

Bacterial Strain

#141407

Purpose

LacI-deficient E. coli host bacterium that produces β-galactosidase constitutively

Depositor

Antal Kiss

Article

Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

Bacterial Resistance

None

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only
MG1655-OptoCre-cat-P-R

Bacterial Strain

#188479

Purpose

Cre-inducible activation of catfor chloramphenicol resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Bacterial Resistance

None

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecB

Bacterial Strain

#176582

Purpose

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP5(nth-ydgR)

Bacterial Strain

#110245

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
CLM24 ∆lpxM

Bacterial Strain

#132780

Purpose

CLM24 strain with lpxM deletion

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Bacterial Resistance

None

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only
BL21ΔBC

Bacterial Strain

#102263

Purpose

lamB, ompC double knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

None

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
TB204 △metA

Bacterial Strain

#230039

Purpose

Fluorescently labelled (GFP) E. coli, auxotrophic for methionine

Depositor

Martin Ackermann, Olga Schubert

Article

Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
RF2

Bacterial Strain

#62070

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC gene deleted

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
C321

Bacterial Strain

#48999

Purpose

Recoded E. coli MG1655 strain with wt UAG termination function (RF1 is still present)

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Ampicillin

Species

Escherichia coli

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
39R861+

Bacterial Strain

#119737

Purpose

Six stable plasmids can be used as size standards

Depositor

Ruth Hall

Article

Moran et al Plasmid. 2019 Mar;102:6-9. doi: 10.1016/

Bacterial Resistance

None

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only
MM39

Bacterial Strain

#42894

Depositor

Bryan Berger

Article

Su et al J Biol Chem. 2012 Jul 22.

Bacterial Resistance

Streptomycin

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
DH10GFP

Bacterial Strain

#109392

Purpose

DH10B with a "capacity monitor" inserted in the lambda phage integration site.

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Bacterial Resistance

Kanamycin

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only
BL21ΔCF

Bacterial Strain

#102265

Purpose

ompC, ompF double knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

None

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
BL21ΔACF

Bacterial Strain

#102268

Purpose

ompA, ompC, ompF triple knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

None

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP ompT- rne-

Bacterial Strain

#182777

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
BL21ΔA

Bacterial Strain

#102256

Purpose

ompA deletion strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

None

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
HL 770

Bacterial Strain

#60369

Purpose

Lim Lab Strain

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Bacterial Resistance

None

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
DH10B-M2.NmeMC58II

Bacterial Strain

#114268

Purpose

The strain expresses M2.NmeMC58II methylase and zeocin selection marker from the arsB locus.

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Bacterial Resistance

Bleocin (Zeocin)

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only
rApoI

Bacterial Strain

#156459

Purpose

An E. coli strain that expresses rat APOBEC1. Used as a control strain for mutagenesis experiments with MutaT7.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Bacterial Resistance

Streptomycin

Species

Rattus norvegicus

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only
BLIM cells

Bacterial Strain

#35609

Depositor

Kathleen Matthews

Article

Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

Bacterial Resistance

None

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
drApo1–T7

Bacterial Strain

#156461

Purpose

An E. coli strain that expresses an inactive variant of MutaT7. Used as a control strain for mutagenesis experiments with MutaT7.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Bacterial Resistance

Streptomycin

Species

Rattus norvegicus

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only
ML3

Bacterial Strain

#61907

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Kanamycin

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
TB205 △ilvA

Bacterial Strain

#230042

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for isoleucine

Depositor

Martin Ackermann, Olga Schubert

Article

Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △ilvA

Bacterial Strain

#230041

Purpose

Fluorescently labelled (GFP) E. coli, auxotrophic for isoleucine

Depositor

Martin Ackermann, Olga Schubert

Article

Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB205 △metA

Bacterial Strain

#230040

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for methionine

Depositor

Martin Ackermann, Olga Schubert

Article

Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
SKA974

Bacterial Strain

#80403

Purpose

JT2-based E. coli host strain for optogenetic control of cell growth.

Depositor

Mustafa Khammash

Article

Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

Bacterial Resistance

Kanamycin

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
nuc5-

Bacterial Strain

#40804

Depositor

George Church

Article

Mosberg et al PLoS One. 2012;7(9):e44638. Epub 2012 Se

Bacterial Resistance

Ampicillin

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
ML8

Bacterial Strain

#61909

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. argH gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Kanamycin

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
Strain KJ1C

Bacterial Strain

#21874

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Bacterial Resistance

Tetracycline

Available Since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only
HL 716

Bacterial Strain

#60368

Purpose

Lim Lab Strain

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Bacterial Resistance

None

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
DH5alpha::DddI

Bacterial Strain

#179631

Purpose

For expression of toxic DddA

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Bacterial Resistance

Gentamicin

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only
Strain CSH100

Bacterial Strain

#21875

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Bacterial Resistance

None

Available Since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only
LC-E03

Bacterial Strain

#78552

Purpose

E. coli strain K-12 MG1655, pTet--Cas9 cassette integrated at 186 primary attB site.

Depositor

David Bikard

Article

Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

Bacterial Resistance

None

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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