159,371 results

1. IFI16-FL (pcDNA3-FLAG)

   Plasmid

   #35064

   Depositor

   Cheryl Arrowsmith

   Article

   Liao et al Structure. 2011 Mar 9;19(3):418-29.

   Insert

   IFI16 (IFI16 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

2. AAVS1-MAFB-IRF8-CEBPA

   Plasmid

   #190720

   Purpose

   For microglia differentiation from iPSC

   Depositor

   Li Gan

   Article

   Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

   Insert

   MAFB-IRF8-CEBPA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-CW3SL-EGFP

   Plasmid

   #61463

   Purpose

   AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Halo_aTubulin-pTwistCMVBetaGlobWPRENeo

   Plasmid

   #221901

   Purpose

   Expresses Halo7 fused to alpha tubulin

   Depositor

   Janelia Integrative Imaging

   Insert

   Halo7-aTubulin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCI-ASC-HA

   Plasmid

   #41553

   Depositor

   Kate Fitzgerald

   Article

   Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

   Insert

   ASC (PYCARD Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate-early enhancer/promoter

   Available since

   Dec. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT3-EF1a-YapS127A

   Plasmid

   #46049

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S127A

   Promoter

   EF1a

   Available since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

7. GFP-TREX1

   Plasmid

   #27219

   Depositor

   Judy Lieberman

   Article

   Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Jan. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRN120

   Plasmid

   #160696

   Purpose

   BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Inserts

   CmYLCV::Luc+::AtHSP
   Nos::WUS2::PinII
   AtUbi10::STM::35S
   

   Use

   Luciferase; Gemini viral replicon, plant developm…

   Tags

   Expression

   Mutation

   Promoter

   AtUbi10, CmYLCV, and Nos

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-N1-PARP1

   Plasmid

   #211578

   Purpose

   CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ef1a_Large T-antigen_Ires_Puro

    Plasmid

    #18922

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    Large T Antigen (SV40gp6 Simian virus 40)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGSKU

    Plasmid

    #72243

    Purpose

    Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    kanMX4
    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p285 TIR1_HA_mH11

    Plasmid

    #164855

    Purpose

    constitutive TIR1 expression vector for mouse H11 locus

    Depositor

    Philip Jordan

    Article

    Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

    Insert

    OsTIR1
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Red Glifon 3000

    Plasmid

    #163116

    Purpose

    Red fluorescent protein-based glucose sensor for mammalian cells with lower affinity

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon 3000
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDRM210 LGP (Luciferase GFP Puro)

    Plasmid

    #174722

    Purpose

    Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    eGFP
    pac
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and F2A linker

    Expression

    Mammalian

    Mutation

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RfxCas13d-backbone

    Plasmid

    #165078

    Purpose

    Expression of RfxCas13d sgRNAs from a U6 promoter

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    Scaffold gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pICE-FLAG-NAT10-siR-WT

    Plasmid

    #59365

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Larrieu et al Science. 2014 May 2;344(6183):527-32. do

    Insert

    human NAT10 (NAT10 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Mutations to render cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid

    #138147

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mEmerald-Mito-7

    Plasmid

    #54160

    Purpose

    Localization: Mitochondria, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pEGFP-C1-ER alpha

    Plasmid

    #28230

    Purpose

    Mammalian expression of Estrogen Receptor alpha fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

    Insert

    Estrogen Receptor alpha (ESR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-pBAD

    Plasmid

    #54630

    Purpose

    Localization: Protein Expression Vector , Excitation: 587, Emission: 610

    Depositor

    Michael Davidson, Nathan Shaner, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Type

    Empty backbone

    Use

    Tags

    6xHis and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGWB-cLUC

    Plasmid

    #174051

    Purpose

    Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

    Depositor

    Alberto Macho

    Article

    Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHR-PP7-2xmCherry-CAAX

    Plasmid

    #74925

    Purpose

    Expresses PP7-2xmCherry-CAAX

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    PP7-2xmCherry-CAAX
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA6 asyn WT

    Plasmid

    #107425

    Purpose

    Mammalian expression of human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti CMV rtTA3 Hygro (w785-1)

    Plasmid

    #26730

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLBS-mScarlet

    Plasmid

    #129337

    Purpose

    cytosol marker, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    mScarlet (Addgene 85042)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40

    Plasmid

    #105545

    Purpose

    AAV expression of EGFP-Cre from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP-Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HI

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. rAAV9-CAP-in-cis-Lox-AAV

    Plasmid

    #205990

    Purpose

    rAAV9 cap acceptor vector for CREATE libraries

    Depositor

    Viviana Gradinaru

    Article

    Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

    Insert

    rAAV9 cap acceptor vector for CREATE libraries
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Deletion between XbaI and AgeI sites

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-sfGFP

    Plasmid

    #125747

    Purpose

    sfGFP without iCreERT2 fusion

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EB1-tdTomato

    Plasmid

    #50825

    Purpose

    expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

    Depositor

    Erik Dent

    Insert

    EB1 (Mapre1 Mouse)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pON.mCherry

    Plasmid

    #84821

    Purpose

    Constitutive Expression plasmid with mCherry fluorescent protein

    Depositor

    Howard Shuman

    Article

    Gebhardt et al PLoS One. 2017 Mar 3;12(3):e0173116. doi

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac Promoter

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge