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Bacterial Expression

29,771 results

Showing: 7501 - 7550 of 29771 results

  7. pDSP33

    Plasmid
    #199375
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Available since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199375-plasmid-map-sequence-id-391013

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  8. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Available since
    May 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    53225-plasmid-map-sequence-id-82901

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  10. pDule2-3-nitroTyrosine (5B)

    Plasmid
    #85499
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85499-plasmid-map-sequence-id-169856

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  12. DOT1L

    Plasmid
    #40736
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (DOT1L Human)
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. DOT1L

    Plasmid
    #36196
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (GeneScript Synthesized) (DOT1L Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-420
    Promoter
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pSC201plus

    Plasmid
    #164227
    Purpose
    Integrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression
    Depositor
    Silvia Cardona
    Article
    Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Rhamnose-inducible
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164227-plasmid-map-sequence-id-325697

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  22. pOPINO_H3

    Plasmid
    #171927
    Purpose
    Bacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_H3
    Use
    Tags
    His6
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171927-plasmid-map-sequence-id-338639

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  32. pHH0103 BAG3 WW domain

    Plasmid
    #104266
    Purpose
    Bacterial expression of WW domain from BAG3
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    BAG3 WW domain (BAG3 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104266-plasmid-map-sequence-id-203492

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  33. T-IL2-12

    Plasmid
    #166564
    Purpose
    scFv of a human scaffold targeting Interleukin-2. Antigen coverage aa 21-153 of 153, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166564-plasmid-map-sequence-id-327819

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  37. pMflET-o4

    Plasmid
    #101314
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    ereB resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    101314-plasmid-map-sequence-id-198386

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  43. pGGA_RT_pLexA

    Plasmid
    #136961
    Purpose
    Goldengate/Greengate module for generation of beta-estradiole inducible genes.
    Depositor
    Klaus Theres
    Insert
    pLexA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136961-plasmid-map-sequence-id-269088

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  44. pET22b_EcSSB_2

    Plasmid
    #78204
    Purpose
    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
    Depositor
    Martin Webb
    Article
    Fili et al Nucleic Acids Res. 2010 Jul;38(13):4448-
    Insert
    ssb (W88C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    W88C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78204-plasmid-map-sequence-id-151737

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  45. pGEX-2T-Cdc42-T17N

    Plasmid
    #12970
    Depositor
    Gary Bokoch
    Insert
    Cdc42 DN (CDC42 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    T17N Dominant negative
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  48. pTH385-GST-TEV-SUP35

    Plasmid
    #29744
    Depositor
    Tobias von der Haar
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    wild type
    Promoter
    Available since
    Aug. 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    29744-plasmid-map-sequence-id-13828

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  49. pSL-1

    Plasmid
    #180422
    Purpose
    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.
    Depositor
    Nancy Moran
    Article
    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119
    Insert
    Red Chromprotein gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    180422-plasmid-map-sequence-id-357215

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Showing: 7501 - 7550 of 29771 results