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32,669 results

Showing: 7501 - 7550 of 32669 results

  3. pET28-Nsp14-TEV-Strep

    Plasmid
    #154953
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
    Tags
    TEV-Strep
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154953-plasmid-map-sequence-id-307852

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  4. As0

    Plasmid
    #123158
    Purpose
    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J109, ParsR-ABS67, PhrpLE, PrinA
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123158-plasmid-map-sequence-id-243982

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  5. pDIV303

    Plasmid
    #204978
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    amdS
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    two silent mutations (nt177T>C, nt879C>T)
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204978-plasmid-map-sequence-id-415579

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  6. pDSP31

    Plasmid
    #199373
    Purpose
    Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199373-plasmid-map-sequence-id-390590

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  10. pET21b-MBPx-MBPx-SpyTag003

    Plasmid
    #216291
    Purpose
    Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    MBPx-MBPx-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 and SpyTag003
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216291-plasmid-map-sequence-id-443903

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  11. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid
    #194469
    Purpose
    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Article
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Expression
    Bacterial
    Available Since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    194469-plasmid-map-sequence-id-384873

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  15. pelB-MBP-BB0238-ss

    Plasmid
    #137046
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0238: lacks the BB0238 signal peptide
    Promoter
    T7lac
    Available Since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    137046-plasmid-map-sequence-id-292071

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  17. pRPCN

    Plasmid
    #193726
    Purpose
    Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193726-plasmid-map-sequence-id-381907

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  23. pETSUMO2_Bacteroidetes-bGSDM-TEV

    Plasmid
    #222101
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Bacteroidetes-bGSDM-TEV ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    TEV protease site replaces residues E242–G248
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222101-plasmid-map-sequence-id-440273

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  24. pTPH413

    Plasmid
    #185728
    Purpose
    Bacterial PAM profiling of adenine base editor variants
    Depositor
    David Liu
    Article
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Inserts
    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…
    Available Since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185728-plasmid-map-sequence-id-364088

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  26. pHL 1405

    Plasmid
    #53010
    Purpose
    pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    DsrA sRNA
    hfq
    sodB
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  31. pGEM-NLS-Cre-2A-mCherry-FKF

    Plasmid
    #119868
    Purpose
    Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Tags
    mCherry
    Expression
    Bacterial
    Available Since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119868-plasmid-map-sequence-id-240979

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  34. N-BLa 1.2

    Plasmid
    #88999
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    N-BLa 1.2 fusion protein
    Tags
    Flag
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    May 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    88999-plasmid-map-sequence-id-179971

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  35. C-BLa 1.2

    Plasmid
    #89000
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    C-BLa 1.2 fusion protein
    Tags
    Flag
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    89000-plasmid-map-sequence-id-177513

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  37. FLuc-N-HiBiT

    Plasmid
    #207117
    Purpose
    Bacterially expressed HiBiT-FLuc for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    luciferin 4-monooxygenase
    Tags
    HiBiT Tag and His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207117-plasmid-map-sequence-id-417172

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  42. ASSA

    Plasmid
    #42368
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ASSA (ASS1 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  48. pFGL815

    Plasmid
    #52322
    Purpose
    A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.
    Depositor
    Naweed Naqvi
    Article
    Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10
    Type
    Empty backbone
    Use
    Agrobacterium
    Expression
    Bacterial
    Available Since
    July 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52322-plasmid-map-sequence-id-81025

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  50. pJJS007

    Plasmid
    #188330
    Purpose
    PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    mIAA7::wrmScarlet::Linker
    Expression
    Bacterial
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188330-plasmid-map-sequence-id-370746

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Showing: 7501 - 7550 of 32669 results