30,008 results
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Plasmid#222105PurposeA ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.DepositorInsertvirA
UseTagsExpressionBacterial and PlantMutationPromotervirA promoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
eGFP-luciferase-C1
Plasmid#163523PurposeExpresses both eGFP and firefly luciferase in mammalian cellsDepositorInsertFirefly luciferase
UseLuciferaseTagseGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRBN_ΔHBD
Plasmid#232779PurposeExpresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domainDepositorInsertCereblon (CRBN Human)
UseTagsMBP-His6-TEVExpressionBacterialMutationCRBN (residues 47 to 193 and 249 to 436) with GNG…PromoterT7Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-TPNOX
Plasmid#87853PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertTPNOX
UseTagsC-terminal FLAG tag with a linkerExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cas9-His
Plasmid#98158PurposePurification of Cas9 proteinDepositorInsertNLS-Cas9-NLS
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEM415-ldhL-mRFP1
Plasmid#99842PurposeExpression of RFP in LactobacillusDepositorInsertmonomeric red fluorescent protein
UseTagsFLAGExpressionBacterialMutationPromoterldhlAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP6-RNAP
Plasmid#48143PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylADepositorInsertrna polymerase SP6
UseSynthetic BiologyTagsExpressionBacterialMutationV314A (numbering based on NCBI Sequence ID: NP_85…PromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-Cas9-6xHis
Plasmid#62374PurposeExpression of Cas9-6xHis in bacterial cellsDepositorInsertCas9_6xHis
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tn7 helper plasmid
Plasmid#141161PurposeTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoterDepositorInsertTn7 transposon machinery
UseTagsExpressionBacterialMutationPromoterArabinose-inducible pBADAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)
Plasmid#218509Purposeintegrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombinationDepositorInsertmKate2
UseTagsssrA degradation tagExpressionBacterialMutationDeletion of Plac_mrfp cassette, introduction of a…PromoterP23Available sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP566
Plasmid#222724PurposepNP561 - IS621 WT Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19 Giles integrase
Plasmid#170049PurposepUC19 with Giles integraseDepositorInsertGIles Integrase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-dddAI
Plasmid#186559PurposeBroad host range regulated expression of the DddA immunity determinant (DddAI)DepositorInsertdddAI
UseTagsExpressionBacterialMutationPromotertacAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pwtCas9-bacteria
Plasmid#44250PurposeaTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdownDepositorInsertwild-type Cas9
UseCRISPRTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI3M-TBD
Plasmid#113077PurposeExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI3M-TBD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM3D(Gq)
Plasmid#45547PurposeExpresses hM3D (Gq) neuronal activatorDepositorInserthM3D (Gq)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMyC
Plasmid#42192DepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterColE1Available sinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD
Plasmid#98669PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43DepositorInsertTDP-43 (TARDBP Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sfp pet29b C-terminal His Tag
Plasmid#75015PurposeExpresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACPDepositorInsertSfp
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only