Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,156 results

Showing: 1041 - 1080 of 32156 results

  2. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

    Enlarge

  3. pBHM2644

    Plasmid
    #229528
    Purpose
    Carries AID* tag, empty sgRNA, and NATR marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - NATR
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229528-plasmid-map-sequence-id-459927

    Enlarge

  4. pBZCas13b

    Plasmid
    #89898
    Purpose
    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89898-plasmid-map-sequence-id-178222

    Enlarge

  7. pZH509

    Plasmid
    #102664
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102664-plasmid-map-sequence-id-199389

    Enlarge

  11. pGM1192

    Plasmid
    #115683
    Purpose
    E. coli-Streptomyces shuttle vector for gene expression
    Depositor
    Günther Muth
    Insert
    mCherry, aac(3)IV, tsr
    Tags
    Strep-tagII on mCherry, codon optimized
    Expression
    Bacterial
    Mutation
    mCherry codon optimized for Streptomyces
    Promoter
    SF14*
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    115683-plasmid-map-sequence-id-237128

    Enlarge

  15. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available Since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

    Enlarge

  18. GPX4

    Plasmid
    #38797
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX4 (GPX4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    SeCys73 -> Gly
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38797-plasmid-map-sequence-id-428950

    Enlarge

  19. YopH (residues 164-468)

    Plasmid
    #79749
    Purpose
    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    YopH (residues 164-468)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79749-plasmid-map-sequence-id-443904

    Enlarge

  24. OmpF

    Plasmid
    #213135
    Purpose
    Expresses outer membrane protein F
    Depositor
    Sebastian Hiller
    Insert
    OmpF (23-362) (ompF E.coli (K12))
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213135-plasmid-map-sequence-id-422154

    Enlarge

  25. pAIP4_3RZY

    Plasmid
    #234156
    Purpose
    Heterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3RZY (FABP4 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234156-plasmid-map-sequence-id-464122

    Enlarge

  30. pCB-CBEv2

    Plasmid
    #221139
    Purpose
    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Bacterial
    Promoter
    lacIq-Ptac
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221139-plasmid-map-sequence-id-437031

    Enlarge

  37. pKL004

    Plasmid
    #98920
    Purpose
    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.
    Depositor
    Joel Kralj
    Article
    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11
    Insert
    GCaMP6f tethered to mRuby
    Tags
    His and mRuby3
    Expression
    Bacterial
    Promoter
    BBa_J23118 - Strong Constitutive
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98920-plasmid-map-sequence-id-189873

    Enlarge

  38. pDM028

    Plasmid
    #216809
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216809-plasmid-map-sequence-id-430135

    Enlarge

Showing: 1041 - 1080 of 32156 results