32,156 results
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Plasmid#171081PurposeCo-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYCDepositorTagsGST and HisExpressionBacterialPromoterT5 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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pUC57-Curlcake1
Plasmid#139340PurposeExpresses Curlcake1 (CC1) in E.coliDepositorInsertCurlcake1 with T7 promoter
ExpressionBacterialPromoterT7Available SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Curlcake2
Plasmid#139341PurposeExpresses Curlcake2 (CC2) in E.coliDepositorInsertCurlcake2 with T7 promoter
ExpressionBacterialPromoterT7Available SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Curlcake4
Plasmid#139343PurposeExpresses Curlcake4 (CC4) in E.coliDepositorInsertCurlcake4 with T7 promoter
ExpressionBacterialPromoterT7Available SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_3xNLS_SpCas9_protein_expression
Plasmid#114365PurposeProtein expression plasmid for 3xNLS SpCas9 in pET-21a backboneDepositorInsert3xNLS_SpCas9
UseCRISPRTagsNLSExpressionBacterialPromoterT7Available SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBA560
Plasmid#186236PurposepTet-eLbuCas13a crRNA entry vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-NLS-Cas9-6xHis
Plasmid#62934PurposeExpression of NLS-Cas9-6xHis in bacterial cellsDepositorInsertNLS-Cas9-6xHis
Tags6xHis tag and NLSExpressionBacterialPromoterT7Available SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b_Bst-LF
Plasmid#153313PurposeBacterial expression of the Bst Large FragmentDepositorInsertBst Large Fragment
ExpressionBacterialAvailable SinceJune 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDM030
Plasmid#216810PurposeVector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.DepositorTypeEmpty backboneUseCRISPR; Fungal expressionExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-PRC1
Plasmid#167539PurposeFor expression of His6-TEV-PRC1 in bacteria.DepositorAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQacR-Q1
Plasmid#74689PurposeQacR-Q1 gateDepositorInsertPTac-RBSQ1-QacR-PQacR-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSmartBAC-S
Plasmid#107268PurposeBroad host range bacterial artificial chromosome vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Claspin-FL-wt
Plasmid#31621DepositorAvailable SinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCK314
Plasmid#110545PurposeModified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteriaDepositorInsertsModified PrhaBAD (CRP-binding sites removed)
yfp
rhaS
ExpressionBacterialAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(-30)GFP
Plasmid#62936PurposeExpression of 6xHis-(-30)GFP in bacterial cellsDepositorInsert(-30)GFP
Tags6xHis tagExpressionBacterialPromoterT7Available SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRsetB-PercevalHR
Plasmid#49081PurposeBacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.DepositorInsertPercevalHR
Tags7xHisExpressionBacterialAvailable SinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETM33_IRF3_SMAD/FHA
Plasmid#178479PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7(gfcC)
Plasmid#176197PurposegfcC protein expressionDepositorInsertgfcC
Tags6xHis-TeVExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJ915
Plasmid#69090PurposeExpress Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLSDepositorInsertcas9
TagsMBP, hexa His, and two SV40 NLSExpressionBacterialPromoterT7Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PE-bacteria
Plasmid#172715PurposeExpression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editingDepositorInsertCas9n-linker-M-MLV2
ExpressionBacterialMutationH840A of Cas9Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP wild type
Plasmid#174075PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
TagsHis-6ExpressionBacterialPromoterT7Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY71-PT7-RiboJ-sfGFP-MGapt
Plasmid#129119PurposeExpresses a fluorescent reporter for the quantification of cell-free protein expression.DepositorInsertSuperfolder GFP with Malachite Green aptamer
UseSynthetic BiologyTagsHis6 and RiboJ InsulatorExpressionBacterialPromoterT7Available SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pARC8-LambdaRedBeta-EcSSB
Plasmid#162572PurposeArabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.DepositorInsertsLambda Red-Beta
E. coli SSB
ExpressionBacterialPromoterpBADAvailable SinceMay 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEmal10H – MBP-EWS_300-447 (RGG1+RRM)
Plasmid#238369PurposeExpresses the RGG1-RRM (300-447) region from the RNA-binding protein EWSDepositorInsertEWSR1 (EWSR1 Human)
Tags10xHis-MBPExpressionBacterialMutationdeleted amino acids 1-299, 448-638PromoterT7Available SinceSept. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY18
Plasmid#221708PurposeEmpty Cas9 vector for multiplex gene editing in fungiDepositorTypeEmpty backboneUseCRISPR; AspergillusExpressionBacterialAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
FDA60
Plasmid#45606DepositorInsertFDA60
Tags6xHisExpressionBacterialAvailable SinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET22b-6h-GST-TEVp
Plasmid#172887PurposeCodon optimized (E. coli) TEVp fused with GST and his-tagged for purificationDepositorInsertTEV protease
TagsGST solubility tag and HHHHHHExpressionBacterialPromoterT7 promoterAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS-QUE7mu
Plasmid#129311PurposeLow concentration ATP measurementDepositorInsertQUEEN-7mu
ExpressionBacterialAvailable SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
T777T
Plasmid#113082PurposeEnhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.DepositorTypeEmpty backboneUseRNAiExpressionBacterialAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-EGFP-PRC1
Plasmid#167540PurposeFor expression of His6-TEV-EGFP-PRC1 in bacteria.DepositorAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
OPEN HLA-A*02:01-BirA
Plasmid#215569PurposeE. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.DepositorInsertOPEN HLA-A*02:01 (HLA-A Human)
TagsBirAExpressionBacterialMutationGlycine 120 to CysteinePromoterT7Available SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-sgRNA-RFP
Plasmid#166005PurposeBacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)DepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
CHIKV eGFP-Zeo replicon
Plasmid#249289PurposeSP6-driven CHIKV_LR2006 OPY1 replicon with eGFP-Zeocin reporter selection cassetteDepositorInsertCHIKV LR2006-OPY1 replicon harboring nonstructural genes (nsp1-nsp4) and eGFP-Zeocin ORF in place of structural genes
ExpressionBacterialMutationstructural polyprotein open reading frame deleted…PromoterSP6Available SinceJan. 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-pBAD
Plasmid#54622PurposeLocalization: Protein Expression Vector , Excitation: 488, Emission: 507DepositorHas ServiceCloning Grade DNATypeEmpty backboneTags6xHis and mEGFPExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZac2.1 gfaABC1D-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176741PurposeExpresses membrane bound biotin ligase, BioID2, in astrocytesDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin WT
Plasmid#12647DepositorAvailable SinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
elavl3:hArr-TEV;he1.1:YFP
Plasmid#218855PurposeD. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.DepositorInserthArr
Tagshuman Arrestin-Tobbaco Etch Virus protease fusion…ExpressionBacterialAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMevT
Plasmid#17815DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-proinsulin-msGFP2
Plasmid#160466PurposeEncodes a fusion of human proinsulin and the monomeric superfolder GFP2DepositorInsertproinsulin-msGFP2
ExpressionBacterialPromoterT5Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Ezr-BioID2-HA
Plasmid#176744PurposeExpresses Ezrin fused with BioID2 at the C-terminus in astrocytesDepositorInsertEzr-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only