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31,059 results

Showing: 1321 - 1360 of 31059 results

  3. pDNA2.0 6H-TEV-KRAS G13D

    Plasmid
    #111851
    Purpose
    Expresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G13D (KRAS Human)
    Tags
    6xHis-tag and TEV protease cleavage sequence
    Expression
    Bacterial
    Mutation
    Changed Glycine 13 to Aspartic Acid
    Promoter
    T5
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111851-plasmid-map-sequence-id-213465

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  16. pKB225

    Plasmid
    #170015
    Purpose
    Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Peptide display protein with no C-terminal peptide
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    Nov. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    170015-plasmid-map-sequence-id-335287

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  18. 6BHD (SETDB1)

    Plasmid
    #119955
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    SETDB1 (SETDB1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 190-410
    Promoter
    T7
    Available Since
    Feb. 1, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119955-plasmid-map-sequence-id-234347

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  19. pCDF-mcherry1

    Plasmid
    #87144
    Purpose
    Constitutive E. coli mcherry Expression
    Depositor
    Michael Lynch
    Insert
    mcherry
    Expression
    Bacterial
    Promoter
    Constitutive proD
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87144-plasmid-map-sequence-id-176832

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  23. pZP06

    Plasmid
    #232320
    Purpose
    pZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232320-plasmid-map-sequence-id-462279

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  25. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

    Plasmid
    #189752
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Article
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet3
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    189752-plasmid-map-sequence-id-375290

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  34. pExp-MBP

    Plasmid
    #112568
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-MBP and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112568-plasmid-map-sequence-id-217224

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Showing: 1321 - 1360 of 31059 results