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32,156 results

Showing: 1321 - 1360 of 32156 results

  11. pDM043

    Plasmid
    #216813
    Purpose
    Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mScarlet3
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216813-plasmid-map-sequence-id-430146

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  12. HA-HA-AR-NTD-DBD

    Plasmid
    #171236
    Purpose
    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain (AR Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171236-plasmid-map-sequence-id-336032

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  13. Pet28a_Synthetic_Human_H2AZ.1

    Plasmid
    #42629
    Depositor
    Joe Landry
    Insert
    Human H2AZ.1 (H2AZ1 Synthetic, Codon Optimized, Human)
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Codon Optimized
    Promoter
    T7
    Available Since
    March 21, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42629-plasmid-map-sequence-id-434040

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  15. KPC2-pNic-Bsa4

    Plasmid
    #231082
    Purpose
    expresses Klebsiella pneumoniae carbapenemase-2 (KPC-2) under T7 with His tag
    Depositor
    Josh Beckham
    Insert
    carbapenemase-2 (kpc2 )
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    231082-plasmid-map-sequence-id-462636

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  16. pExp-Trx

    Plasmid
    #112577
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-TrxA and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112577-plasmid-map-sequence-id-217212

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  17. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid
    #178043
    Purpose
    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP
    Depositor
    Han Xiao
    Article
    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1
    Inserts
    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    Tags
    His tag
    Expression
    Bacterial
    Available Since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    178043-plasmid-map-sequence-id-359586

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  24. pDest-565

    Plasmid
    #11520
    Purpose
    Gateway destination vector for bacterial expression; His,GST tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    6xHis and GST
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11520-plasmid-map-sequence-id-324017

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  25. pPEPX-P3-sgRNAluc

    Plasmid
    #85590
    Purpose
    Integrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template for
    Depositor
    Jan-Willem Veening
    Article
    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do
    Insert
    sgRNA targeting firefly luciferase encoding gene
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    P3
    Available Since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85590-plasmid-map-sequence-id-169513

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  26. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Article
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163148-plasmid-map-sequence-id-331409

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  30. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid
    #114070
    Purpose
    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114070-plasmid-map-sequence-id-220511

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  31. pJJS006

    Plasmid
    #188329
    Purpose
    PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::mNeonGreen::mIAA7
    Expression
    Bacterial
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188329-plasmid-map-sequence-id-370757

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  38. pPaGE Pyl TAG FliC T248TAG

    Plasmid
    #160089
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea
    Inserts
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Flagellin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Changed Threonine 248 to TAG stop-codon.
    Promoter
    Pseudomonas aeruginosa Leu-tRNA native promoter a…
    Available Since
    June 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160089-plasmid-map-sequence-id-314779

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  39. pET21a-Streptavidin-Dead

    Plasmid
    #20859
    Purpose
    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Dead
    Expression
    Bacterial
    Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A
    Available Since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20859-plasmid-map-sequence-id-220527

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  40. pGPΩTp

    Plasmid
    #130660
    Purpose
    Insertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.
    Depositor
    Miguel Valvano
    Article
    Flannagan et al Infect Immun. 2007 Apr;75(4):1679-89. Ep
    Insert
    Ω trimethoprim cassette
    Expression
    Bacterial
    Mutation
    Addition of Ω sites next to trimethoprim cassette…
    Promoter
    dhfr promoter
    Available Since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    130660-plasmid-map-sequence-id-258172

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Showing: 1321 - 1360 of 32156 results