31,059 results
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Plasmid#65030PurposeGmR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
ExpressionBacterialAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
EcPhnD90.L1∆∆AD.L297RL301R.pRSET
Plasmid#33376Purposea single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFPDepositorInsertphnD based Phosphonate Biosensor (phnD E. coli)
Tags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted in place of…PromoterT7Available SinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRAV24
Plasmid#67716PurposeParental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purificationDepositorInsertTranscription cassette for 3'-tagged RNAs
ExpressionBacterialPromoterT7 RNA polymeraseAvailable SinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-GST-Tev-Af1521-myc
Plasmid#196239PurposeN-terminal GST fusion of Af1521(WT) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminusDepositorInsertN-terminal GST fusion of Af1521(WT) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
TagsGST tag and myc tagExpressionBacterialAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28.His.3C.86b.Tau2N4R
Plasmid#226399PurposeExpresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged WT 2N4R tau for recombinant protein production in bacteriaDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-MerTK-PTPN1
Plasmid#197988PurposeExpression of MerTK Kinase Domain (wild-type) in E. coliDepositorExpressionBacterialPromoterT7 promoterAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM31
Plasmid#135491PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3DepositorInserthph SpunH2A/Bpr hph-mCerulean3
TagsmCerulean3ExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512_Mut235
Plasmid#179278PurposeExpresses Br512_Mut235 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
Tags8x His tag; Villin Headpiece 47 amino acid (HP47)…ExpressionBacterialPromoterT7Available SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Kan-LacI
Plasmid#79826PurposeArabinose induced expression of Lac repressorDepositorInsertLacI
TagsHis6ExpressionBacterialPromoteraraBpAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
JC Mad-1
Plasmid#25626DepositorInsertJC Virus Mad-1
ExpressionBacterialAvailable SinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJMP1346
Plasmid#119274Purposeknockdown folA in K. pneumoniaeDepositorInsertsgRNA folA (K. pneumoniae)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST44-AP-1 core
Plasmid#184809PurposeBacterial expression of clathrin adaptor AP-1 core complexDepositorInsertHs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
TagsGST tag and His6ExpressionBacterialMutationAP1S3: S148C; AP1B1: K359R, E476KPromoterT7Available SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIM1773-PRHIII-sfGFP-pNW33N
Plasmid#52217PurposeConstitutive sfGFP expression plasmid for use in Geobacillus and E. coliDepositorInsertPRHIII-sfGFP
ExpressionBacterialPromoterPRHIIIAvailable SinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHyo182
Plasmid#173136PurposeExpresses pheS_A294G in e.coliDepositorInsertpheS_A294G
ExpressionBacterialAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b His6-FKBPF36V
Plasmid#73180PurposeExpressing FKBPF36V protein in washing out shield-1 ligand for FKBPddDepositorAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLG001 (empty vector)
Plasmid#157879PurposeEmpty vector backbone for defense systemsDepositorInsertNone
ExpressionBacterialAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJLG029
Plasmid#192980PurposeBHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purificationDepositorInsertpruR-3xFLAG
Tags3XFLAGExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-RF1-His6
Plasmid#224372PurposeExpress RF1 protein for his-tagged purificationDepositorAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHN922
Plasmid#178975PurposeExpression of GST-His8-GFP-nanobodyDepositorInsertGST-His8-GFP-nanobody
UseAffinity Reagent/ AntibodyTagsGST, His8ExpressionBacterialPromotertacAvailable SinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBJ3
Plasmid#167132PurposeA kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterFrr promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTCF158 Human beta-2 microglobulin
Plasmid#180457PurposeE. coli expression of class I MHC light chainDepositorInsertHuman beta-2 microglobulin
ExpressionBacterialAvailable SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBR BsmAIM Kan + p15A BsaIR Amp
Plasmid#206208PurposeContains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIRDepositorInsertBsamAIM and BsaIR
ExpressionBacterialAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-sfTq2ox
Plasmid#124220PurposePeriplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,DepositorInsertOmpA-mNG-sfTq2ox
ExpressionBacterialAvailable SinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b_H6-Smt3-AesC-GFP
Plasmid#234486PurposeSuperfolder GFP C-terminally fused to His-Smt3-tagged AesC split inteinDepositorInsertC-terminal Fragment of the Aes123 PolB1 Split Intein
TagsHexahistidine Tag, Superfolder Green Fluorescent …ExpressionBacterialAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ecoli PancACe
Plasmid#215712PurposeExpresses acetyl-CoA biosensor in EcoliDepositorInsertPancACe
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cys-60nmSAH-SPYcatcher
Plasmid#214840PurposeThis plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-60nmSAH-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
ExpressionBacterial and YeastAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-GFP_ColE1
Plasmid#171648PurposeExpresses GFP in BL21(DE3) E. coli. Positive control for expression experiments. Contains ColE1 origin of replication.DepositorInsertT7-LacO-GFPssrA-T7 Stop
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
GPX4
Plasmid#38797PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQFT-mNG
Plasmid#203675PurposepQFT with mNeonGreen under control of PQJDepositorInsertmNeonGreen
ExpressionBacterialPromoterPQJAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP43-NTD
Plasmid#241024PurposeBacterial expression of TDP-43 NTDDepositorInsertTDP43-NTD
Tags6x HisExpressionBacterialMutationchanged N81 to stop codonAvailable SinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG8Ha-SERBP1(149-400)
Plasmid#176516PurposeExpresses the human C-terminal SERBP1 149-400 construct.DepositorInsertSERBP1 149-400 (SERBP1 Human)
TagsHis8 tag, linker, TEV siteExpressionBacterialMutationdeleted amino acids 1-148 and 401-408PromoterT7Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2CT-Cdi
Plasmid#83044PurposeExpresses Corynebacterium diphtheriae Cas9 (CdiCas9)DepositorInsertCdiCas9
TagsHis-MBPExpressionBacterialAvailable SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP1337
Plasmid#119270Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
plRL118
Plasmid#163632PurposeRenilla luciferase reporter plasmid; Giles integrating.DepositorTypeEmpty backboneUseLuciferaseExpressionBacterialPromoterMycobacterial Optimized Promoter (MOP)Available SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBb(RK2)1k-GFPuv
Plasmid#106394PurposeIPTG-inducible trc promoter expressing gfpuv on RK2 originDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2T
Plasmid#15808DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEC-K-CBP_CjeCas9
Plasmid#83043PurposeExpresses Campylobacter jejuni Cas9 (Cje Cas9).DepositorInsertCjeCas9
ExpressionBacterialAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU1_000
Plasmid#186773Purpose* Engineered U1 snRNA plasmid for insertion of guide sequences.DepositorTypeEmpty backboneUseCRISPRExpressionBacterial and MammalianPromoterU1 PromoterAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-K128N
Plasmid#120284PurposeProduce human recombinant FGF2 K128N in DE3 cellsDepositorInsertBasic fibroblast growth factor 2 (FGF2 Synthetic, Human)
Tags6x HisExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only