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32,126 results

Showing: 1721 - 1760 of 32126 results

  2. pEXP5-NT/pLux eGFP

    Plasmid
    #193624
    Purpose
    Inducible expression of eGFP by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL).
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    eGFP
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    pLux
    Available Since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193624-plasmid-map-sequence-id-381997

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  3. pEXP5-NT/pT7 LuxR

    Plasmid
    #193625
    Purpose
    Constitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LuxR
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7 RNAP promoter
    Available Since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193625-plasmid-map-sequence-id-388599

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  4. Py-MDR2-NBD

    Plasmid
    #25590
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Py-MDR2-NBD
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. pD-MSP1D1

    Plasmid
    #161607
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    D-MSP1D1
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1; all tryptophans were replaced b…
    Promoter
    T7
    Available Since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161607-plasmid-map-sequence-id-317653

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  11. pHtrA-TEV-His12

    Plasmid
    #137036
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137036-plasmid-map-sequence-id-275699

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  18. pTYB3-T4gp43(WT)

    Plasmid
    #228203
    Purpose
    Expression in BL21(DE3) of WT T4 DNA polymerase
    Depositor
    Stephen Benkovic
    Insert
    43
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    228203-plasmid-map-sequence-id-472938

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  21. PARP1 FL

    Plasmid
    #169815
    Purpose
    Expresses codon optimised full length His tagged PARP1 in bacterial cells
    Depositor
    David Neuhaus
    Article
    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226
    Insert
    Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Human, Synthetic)
    Tags
    MKHHHHHHMKQ
    Expression
    Bacterial
    Mutation
    Valine 762 mutated to an alanine
    Promoter
    T7 promoter
    Available Since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    169815-plasmid-map-sequence-id-334563

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  22. pDest-566

    Plasmid
    #11517
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    6xHis and MBP
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11517-plasmid-map-sequence-id-299808

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  27. pET-6xHis-(pos28)GFP

    Plasmid
    #89250
    Purpose
    Expresses GFP with +28 theoretical charge in E.Coli
    Depositor
    David Liu
    Insert
    His6-(pos16)-GFP
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    89250-plasmid-map-sequence-id-176151

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  31. pET151-D-TOPO-PARP14-SΔK

    Plasmid
    #216658
    Purpose
    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.
    Depositor
    Herwig Schüler
    Article
    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.
    Insert
    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
    Tags
    6xHis-V5-TEV
    Expression
    Bacterial
    Mutation
    Deletion starting from Q1390 to D1448 and substit…
    Promoter
    T7
    Available Since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216658-plasmid-map-sequence-id-429340

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  35. pBBR15.2-2H12.D11opt-scarRef

    Plasmid
    #221150
    Purpose
    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2.1
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2.1)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221150-plasmid-map-sequence-id-437166

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  37. pGL4.R_TRE_minCMV

    Plasmid
    #211518
    Purpose
    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression
    Depositor
    Justin English
    Article
    Zahm et al Nat Commun. 2024 Nov 28;15(1):10353. doi
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    SV40 and minCMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211518-plasmid-map-sequence-id-419254

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  38. pENV8(GAAA)-GFPuv

    Plasmid
    #70128
    Purpose
    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop
    Depositor
    Robert Batey
    Article
    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:
    Insert
    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70128-plasmid-map-sequence-id-130022

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  39. PGL4.11-COL1A1mTEAD

    Plasmid
    #230916
    Purpose
    Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase- luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    mutant potential TEAD bind elements AGGAAT to CTG…
    Promoter
    COL1A1
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230916-plasmid-map-sequence-id-460667

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Showing: 1721 - 1760 of 32126 results