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32,656 results

Showing: 2451 - 2500 of 32656 results

  5. pHBintE

    Plasmid
    #48136
    Purpose
    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector
    Depositor
    Dieter Jahn
    Article
    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    48136-plasmid-map-sequence-id-69942

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  6. pSW031

    Plasmid
    #231144
    Purpose
    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.
    Depositor
    Philip Cole
    Article
    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021
    Insert
    S. aureus Sortase A mutant
    Tags
    Nostoc punctiform split intein C-terminal fragmen…
    Expression
    Bacterial
    Mutation
    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…
    Promoter
    T7
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231144-plasmid-map-sequence-id-460063

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  11. pD-MSP1D1

    Plasmid
    #161607
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    D-MSP1D1
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1; all tryptophans were replaced b…
    Promoter
    T7
    Available Since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161607-plasmid-map-sequence-id-317653

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  14. T7 promoter + terminators reporter plasmid

    Plasmid
    #156456
    Purpose
    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.
    Depositor
    Matthew D. Shoulders
    Article
    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560
    Inserts
    NeoR/KanR
    TcR
    Expression
    Bacterial
    Mutation
    ATG start codon mutated to ACG
    Promoter
    Em7 and tet
    Available Since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156456-plasmid-map-sequence-id-311167

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  15. pNB22

    Plasmid
    #103059
    Purpose
    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpAp
    Depositor
    Dirk Bumann
    Article
    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-
    Insert
    GFP-OVA
    Tags
    none
    Expression
    Bacterial
    Promoter
    hmpAp
    Available Since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    103059-plasmid-map-sequence-id-199053

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  18. GST-mDia1(FH1FH2DAD)-His

    Plasmid
    #85822
    Purpose
    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.
    Depositor
    Antoine Jégou, Guillaume Romet-Lemonne
    Article
    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc
    Insert
    Protein diaphanous homolog 1 (Diaph1 Mouse)
    Tags
    8*His and GST
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85822-plasmid-map-sequence-id-183287

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  23. pLT 653

    Plasmid
    #59996
    Purpose
    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
    Depositor
    Lisa Timmons
    Article
    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(
    Inserts
    dhc-3 (dhc-3 Nematode)
    klp-16 (klp-16 Nematode)
    Expression
    Bacterial
    Mutation
    partial genomic
    Available Since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    59996-plasmid-map-sequence-id-110769

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  24. pET24a-VHH-myc

    Plasmid
    #109422
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, T7, and myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109422-plasmid-map-sequence-id-211585

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  31. pORTMAGE311B

    Plasmid
    #120418
    Purpose
    Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR
    Depositor
    Csaba Pál
    Article
    Szili et al Antimicrob Agents Chemother. 2019 Aug 23
    Inserts
    MutL E32K
    Lambda Beta
    XylS
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Pm
    Available Since
    Feb. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    120418-plasmid-map-sequence-id-236013

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  36. pDM030

    Plasmid
    #216810
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216810-plasmid-map-sequence-id-430137

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  40. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

    Plasmid
    #218509
    Purpose
    integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mKate2
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Deletion of Plac_mrfp cassette, introduction of a…
    Promoter
    P23
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218509-plasmid-map-sequence-id-438730

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  47. pExp-NusA

    Plasmid
    #112571
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112571-plasmid-map-sequence-id-220666

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Showing: 2451 - 2500 of 32656 results