29,712 results
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Plasmid#64961Purposemini-Tn7 Gateway destination vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits
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p2.0VPI.EGFP
Plasmid#40868DepositorInsertmouse vasopressin promoter
UseAAVTagsExpressionBacterial and InsectMutationPromotermouse vasopressinAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAT588
Plasmid#68907PurposeNanoLuc expressed constiutively from PBT1311 and GH023 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMJ_042
Plasmid#214061PurposeT7 inducible LanTERN (pRSET)DepositorInsertLanTERN
UseTags6x His, T7 Tag, and Xpress(tm) TagExpressionBacterialMutationPromoterT7Available sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
TAF3 PHD_pGEX-6P-2
Plasmid#92100PurposeExpresses GST fusion TAF3 PHD domain proteinDepositorInsertTAF3 PHD domain (TAF3 Human)
UseTagsGST TagExpressionBacterialMutationPromotertac promoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHybrid-Electra1
Plasmid#179474PurposeExpresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cellsDepositorInsertsElectra1
mScarlet-I
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-CBX1-eReader
Plasmid#135089PurposeBacterial expression of engineered CBX1 with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered
UseTags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWUR702
Plasmid#53079PurposeInducible TtAgo expressionDepositorInsertTtAgo
UseTagsStrep-II tagExpressionBacterialMutationPromoterT7Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSR1037
Plasmid#208736PurposeCharacterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)DepositorInsertPSarA1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-3'TwStrep
Plasmid#208053PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.DepositorTypeEmpty backboneUseTagsTwin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKCyR8
Plasmid#149494PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBECKP-spe
Plasmid#117236Purposencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickaseDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEEK2
Plasmid#83492PurposeExpression plasmid for conjugational transfer into cyanobacteriaDepositorTypeEmpty backboneUseSynthetic BiologyTagsStrep-tagExpressionBacterialMutationPromoterPtrc coreAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AviTag-SnoopLigase
Plasmid#105626PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylationDepositorInsertAviTag-SnoopLigase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24b-SUMO-BG2.4
Plasmid#199438Purposeexpresses BAmGreen2.4 in bacterial cells with a SUMO tagDepositorInsertBAMGreen2.4
UseTagshis tag and sumoExpressionBacterialMutationPromoterT7Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-pro-IL1B
Plasmid#183386PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertpro-IL1B (Il1b Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-AR-LBD
Plasmid#171239PurposeMammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)DepositorInsert1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSP573
Plasmid#98796PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV WT
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGL815
Plasmid#52322PurposeA vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.DepositorTypeEmpty backboneUseAgrobacteriumTagsExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
lacI-PT5-gusA-pBAV1k
Plasmid#30501DepositorInsertbeta-glucuronidase (gusA Escherichia coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mVenus NB
Plasmid#103986PurposePlasmid encoding mVenus NBDepositorInsertmVenus NB
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KO (KanR, 186)
Plasmid#45985DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)
Plasmid#29722DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pST-K
Plasmid#44560Purposefor ectopic constitutive expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterUV15 promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMYCO1_SpdCas9_pmcDA1
Plasmid#204615PurposeCRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoidesDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-tdTomato-hphMX6
Plasmid#105157PurposeYeast gene targetingDepositorInserttdTomato-hphMX6
UseTagstdTomatoExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBLADE(FP6*)-mCherry
Plasmid#168048PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.DepositorInsertsBlue light-inducible AraC dimers in Escherichia coli
mCherry
UseTagsExpressionBacterialMutationPromoterJ23101* and PBADAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-1α
Plasmid#214341PurposeProtein overexpression in bacteriaDepositorInsertIL1A (IL1A Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Paxillin
Plasmid#186142PurposeFor expresseion of recombinant paxillin in bacteriaDepositorInsertPXN (PXN Human)
UseTagsGSTExpressionBacterialMutationPaxillin C1997A (silent mutation for cloning)PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbS5c-mCherry-AsLOV2*(543)
Plasmid#202712PurposeExpresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)DepositorInsertmCherry-AsLOV2*(543)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS PKC theta
Plasmid#8426DepositorInsertMouse Protein Kinase C theta (Prkcq Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 22, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn-pOUT
Plasmid#213905PurposeMariner transposon with outward-facing pJ23104 promoterDepositorInsertmariner transposon with kan resistance and outward-facine pJ23104 promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2B
Plasmid#15781DepositorInsertUbiquitin conjugating enzyme UbE2B (UBE2B Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-6xHis mAb
Plasmid#214952PurposeBacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.DepositorInsertsVNp-anti-6xHis mAb heavy chain
VNp-anti-6xHis mAb light chain
UseTagsVNp6ExpressionBacterialMutationPromoterT7Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-human APPL1
Plasmid#22198DepositorInsertAdaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
p15aC-GFP
Plasmid#107862PurposeControl plasmid expressing GFPDepositorInsertGFP
UseTagsmycExpressionBacterialMutationPromoterproDAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
sfTq2ox-PBP5
Plasmid#117960PurposePeriplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)DepositorInsertsfTq2ox-PBP5 (dacA )
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEGF
Plasmid#199231PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertEpidermal growth factor, partial (EGF Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)
Plasmid#182395PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusionsDepositorInsertsVNp-LZ-VenusN154
VNp-LZ-VenusC155
UseTagsVesicle Nucleating peptide (VNp) & Leucine Zi…ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen-Etanercept
Plasmid#182420PurposeBacterial expression of mNeongreen-Etanercept fusionDepositorInsertmNeongreen-Etanercept (TNFRSF1B Human)
UseTagsmNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-HIV RT-6xHis-RBS-prot
Plasmid#159149Purposeexpresses His-tagged HIV reverse transcriptase and untagged HIV proteaseDepositorInsertsHuman immunodeficiency virus 1 (HIV-1) reverse transcriptase
Human immunodeficiency virus 1 (HIV-1) protease
UseTagsHisExpressionBacterialMutationHIV-1 group M/subtype B – BH10 strainPromoterAvailable sinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
L4440_BioBrick-SCR-sgRNA-A
Plasmid#177811PurposeOverexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)DepositorInsertScrambled sgRNA targeting sequences
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD237-3a-2
Plasmid#90957PurposeExpresses ThsR and mCherry constitutively and sfGFP under the PphsA promoterDepositorInsertsThiosulfate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23100, Bba_J23105, and PphsAAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAPB02
Plasmid#65628PurposeA transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilisDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pHBintE
Plasmid#48136PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBK- Ma PylRS WT
Plasmid#197573PurposeExpresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS WT
UseTagsExpressionBacterialMutationPromoterGlnS (constitutive)Available sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR-spondin 1
Plasmid#128844Purposebacterial expression of pR-spondin 1DepositorInsertR-spondin 1 (RSPO1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTmpKm
Plasmid#176227PurposeA template plasmid for amplification of the cI-hok-neo cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, neo
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLRD11
Plasmid#208263PurposeProduction of sclareol in E. coli MEV20DepositorInsertstHMGR
abCAS
ssSS
UseTagsExpressionBacterialMutationabCAS(D537A)PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only