29,729 results
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HPF1-c004
Plasmid#216874PurposeBacterial expression vector for E. coli producing full length HPF1 protein with N-terminal YFP fusionDepositorInsertFull length HPF1 (HPF1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huErCas12a
Plasmid#174685PurposeExpresses 6xHis-MBP tagged ErCas12aDepositorArticleInserthuErCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 6xHisExpressionBacterialMutationQ926R mutation is observed as indicated from the …PromoterT7Available sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-6His-MBP-MpAgo
Plasmid#79249PurposeThis plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expressionDepositorInsertMarinitoga piezophila Argonaute
UseCRISPRTags6XHis-tag + MBP + PreScission Cleavage SiteExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_44
Plasmid#109242PurposepSC101 var2 BbsI cloning vectorDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23105Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCL-GKL1ShortMutPRC-Ug1Lg2starTGA
Plasmid#52347PurposeIntegration cassette to create p1-Short-UL*(TGA)DepositorInsertleu*
UseP1 integration vectorTagsExpressionBacterialMutationchanged S2F, A3V, Q180*PromoterUCS from pGKL2 ORF5Available sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSET-RCaMP1h
Plasmid#42874Purposea single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRubyDepositorInsertRed Fluorescent Calcium binding Protein
UseTagsEK (Enterokinase) Recognition Site, His Tag (6x),…ExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET42b-BE3
Plasmid#87437PurposeExpresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertBE3 (Apobec1 Bacillus phage PBS2, Rat, Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_H-Luc
Plasmid#113924PurposePlasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-LucDepositorInsertH-Luc
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGR618
Plasmid#213786PurposeIntegrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBB-Tv TP-DNAP1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFCM1
Plasmid#64948Purposesource of CmR FRT cassetteDepositorInsertFRT-cat-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD WT
Plasmid#169713PurposeBacterial expression of human hnRNPA1-A Low complexity domain including amino acids 186-320DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYUB28b
Plasmid#37277DepositorTypeEmpty backboneUseMycobacteria shuttle vectorTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pTn7-SCOUT11
Plasmid#200991PurposepLv1-pUC18R6KT-miniTn7-Gm, GmR, ApRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEB1030
Plasmid#22067DepositorTypeEmpty backboneUseTagsT18-FlagExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI
Plasmid#50723Purposeoverexpression of human TPI1 in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pYH78
Plasmid#217386PurposePtrc99A-PAmCherry for bacterial expressionDepositorInsertPAmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-AGO2
Plasmid#24317DepositorInserthuman AGO2 (AGO2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-FLAG-Synaptojanin 1-145
Plasmid#22293DepositorInsertSynaptojanin 1_145 (SYNJ1 Human)
UseTagsFlag and GFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
His_CL7_2NLS_iCas12a-4NLS
Plasmid#222971PurposeBacterial expression of NLS-rich iCas12a constructDepositorInsertHis_CL7_2NLS_iCas12a-4NLS
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-53BP1LCD
Plasmid#204407PurposeE. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tagDepositorInsert53BP1(1208-1977) (TP53BP1 Human)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-2-human RNF146 full length
Plasmid#132610Purposeexpresses human RNF146 full length in E. coliDepositorInsertRNF146 (RNF146 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRTagsExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3035 GST-HPV-18 E6
Plasmid#10850DepositorInsertHPV18 E6 (E6 Human papillomavirus)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pAC-BETAipi-ispA/dxs
Plasmid#178302PurposeProduces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.DepositorInsertsispA
dxs
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
luxB:Venus+luxA/pRSETB Duet
Plasmid#197582PurposeBRET lux luciferase with Venus for bacterial expressionDepositorInsertLuxA LuxBVenus
UseE.coli expressionTagsHis X 6ExpressionBacterialMutationPromoterT7Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 human PP2A B56α full length
Plasmid#132635Purposeexpresses human PP2A B56α full length in E. coliDepositorInsertPP2A B56α
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos3.2 (mEosFP3.2-A69T)
Plasmid#99224PurposeBacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).DepositorInsertpr-mEos3.2
UseTagsExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSV2B
Plasmid#118228PurposeEmpty E. coli - B. burgdorferi shuttle vector, blasticidin S resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRibo EcoHind
Plasmid#36252DepositorTypeEmpty backboneUseSynthetic Biology; Mycobacteria replicating vectorTagsExpressionBacterialMutationPromoterModified Hsp60Available sinceApril 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pet16b-Avi-ubiquitin_rbs_BirA
Plasmid#134897PurposeExpresses HIS-Avi-tagged ubiquitin for recombinant protein productionDepositorInsertsUseTags10HIS-AviTag-PreScission(3C)proteaseExpressionBacterialMutationPromoterRBS and T7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY2
Plasmid#21761DepositorInsertECFP -EYFP linked by flexible linker containing two SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET283xFlagPP7CPHis
Plasmid#28174DepositorInsertPP7 coat protein optimized
UseTags6xhistidine and triple FLAG peptideExpressionBacterialMutationC68A,C73APromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Thioredoxin TEV co-transformation cloning vector (13K-T)
Plasmid#48322DepositorTypeEmpty backboneUseTagsHis6 and ThioredoxinExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
groSL-GFP
Plasmid#109389PurposeGFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpgroSL-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-E. coli Initiation Factor 3 (IF3)
Plasmid#31340DepositorInsertE. coli Initiation Factor 3
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Casp1-His
Plasmid#11809DepositorInsertCaspase-1 (CASP1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pBacPuroR-NeoR
Plasmid#34921DepositorInsertsBacterial promoter::PuroR::bacterial 3p flank
Bacterial promoter::NeoR::bacterial 3p flank
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PCMT1_PCMT
Plasmid#109932PurposeProtein expression and purification of PCMT1_PCMTDepositorInsertPCMT1_PCMT
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAP05
Plasmid#102920PurposeDirect cloning and broad-host-range heterologous expression vectorDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Mm2
Plasmid#197100PurposeTo express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivatiDepositorInsertPylRS variant, Pyl tRNA, kanamycin resistance gene
UseTagsExpressionBacterialMutationLys61, Glu131, Ala306, Phe384, Glu444 (PylRS)PromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
mKO2-pBAD
Plasmid#54555PurposeLocalization: Protein Expression Vector , Excitation: 551, Emission: 565DepositorTypeEmpty backboneUseTags6xHis and mKO2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
IL-2 promoter luciferase
Plasmid#12194DepositorInsertIL-2 promoter (Il2 Mouse)
UseLuciferaseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pC28-LIC-sfGFP-His
Plasmid#165479PurposepET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tagDepositorTypeEmpty backboneUseTagsTEV-GFP-6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
COVID-SARS2 NSP14/10
Plasmid#159613PurposeBacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10DepositorInsertsUseTagsHis6, TEV cleavage site and noneExpressionBacterialMutationCodon-optimized for E. coli expressionPromoter(Bicistronic) and T7 - LacOAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only
