32,069 results
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Plasmid#200191PurposeA TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertblasticidine - P2A - TETon-3G transactivator
UseLentiviralExpressionBacterial and MammalianPromoterUbCAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnCS_SEPT6_SEPT7-TEV-Strep
Plasmid#174499Purposebacterial co-expression of human SEPT6 and of human SEPT7DepositorAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPhlF-P1
Plasmid#74685PurposePhlF-P1 gateDepositorInsertPTac-RBSP1-PhlF-PPhlF-YFP
ExpressionBacterialAvailable SinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCgsgRNA_crtYf
Plasmid#153338PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; SpcrDepositorInsertcrRNA targeting crtYf
UseCRISPRExpressionBacterialAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBad-mCherry-XL (R125K)
Plasmid#188054PurposeExpresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteriaDepositorInsertmCherry-XL (R125K)
Tags6xHisExpressionBacterialMutationdiffers from mCherry by R125K, W143S, I161V, Q163…PromoteraraBADAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huMb2Cas12a
Plasmid#174676PurposeExpresses 6xHis-MBP tagged Mb2Cas12aDepositorArticleInserthuMb2Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-PRM-B0034-geneVI
Plasmid#80858PurposeAccessory plasmid (encodes M13 gene VI)DepositorInsertM13 gene VI
ExpressionBacterialPromoterLambda PrmAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase
Plasmid#231128PurposeExpresses xylanase via a cSAT2.0 schemeDepositorInsertxylanase
TagscSAT2.0ExpressionBacterialPromoterT7Available SinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-ABK
Plasmid#126035PurposeEfficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.DepositorInsertstRNA synthetase 1
tRNA synthetase 2
tRNA for TAG codon
ExpressionBacterialMutationL274M, C313A, Y349FPromoteraraBAD promoter, glnS promoter, and proK promoterAvailable SinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2
Plasmid#79783PurposeExpresses Spinach2 under a T7 promoterDepositorInsertT7-tRNA-Spinach2
ExpressionBacterialPromoterT7Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBCPB+
Plasmid#18940DepositorInsertattP, lacZ, attB
ExpressionBacterialAvailable SinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET Flag TEV LIC cloning vector (1L)
Plasmid#29662DepositorTypeEmpty backboneTagsFlag and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRT498-OtUBD(V203D)
Plasmid#190090PurposeExpresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD(V203D)
Tags6xHis and MBPExpressionBacterialAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-GCaMP6f
Plasmid#67564Purposebacterial expression of a fluorescent calcium reporterDepositorInsertGCaMP6f
TagsHIS-tagExpressionBacterialAvailable SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAP05
Plasmid#102920PurposeDirect cloning and broad-host-range heterologous expression vectorDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21b Apaf1 1-591
Plasmid#26642DepositorInsertApoptotic protease activating factor 1 (APAF1 Human)
TagsHisExpressionBacterialMutationAmino Acids 1-591Available SinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCpf1b-sp
Plasmid#122188PurposeBacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection markerDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI
Plasmid#44182DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7FTrp)RS
Plasmid#177310PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-human p53-(1-393)
Plasmid#24860DepositorAvailable SinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen
Plasmid#182413PurposeBacterial expression of mNeongreenDepositorInsertmNeongreen
ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYC1446
Plasmid#158720PurposeIntegrating plasmid expressing Sth1 Cas9 and the cognate sgRNADepositorInsertSth1 sgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_ABCE1-Halo
Plasmid#175335PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmPAGFP_IRES_puro2
Plasmid#21046DepositorInsertpmPAGFP
ExpressionBacterial and MammalianAvailable SinceMay 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Synaptojanin 1-170
Plasmid#22292DepositorInsertSynaptojanin 1_170 (SYNJ1 Human)
TagsFlagExpressionBacterial and MammalianMutationK334R compared to Genbank ID NM_003895Available SinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
MSP573
Plasmid#98796PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV WT
ExpressionBacterialMutationNoneAvailable SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
PBAD-his6-prkA-pACYC184
Plasmid#41041DepositorInsertprkA
Tags6xHis and T7 epitope tagExpressionBacterialPromoterPBADAvailable SinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX5X.1 PINK1 WT
Plasmid#13321DepositorAvailable SinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
HMGCS2
Plasmid#38984PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMMLV-EGFP-P2A-Puro
Plasmid#160226PurposeExpresses EGFP in mammalian cellsDepositorInsertEGFP-P2A-PuroR
UseRetroviralExpressionBacterial and MammalianPromoterLTRAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET Trx His6 LIC cloning vector (2Tc-T)
Plasmid#37239DepositorTypeEmpty backboneTags6xHis and Thioredoxin (Trx)ExpressionBacterialPromoterT7Available SinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX 6P1 CDK2 (552)
Plasmid#61845Purposebacterial expression of GST CDK2 WTDepositorAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
HSA1_His
Plasmid#202763Purposehuman serum albumin design HSA1, in pET29b with C-terminal 6His-tagDepositorInsertHSA1
TagsHis tagExpressionBacterialPromoterT7Available SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-22b DT 51E/148K
Plasmid#11081DepositorInsertDT
TagsHisExpressionBacterialMutation51E/148K, catalytically inactiveAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIBA3-SpyCatcher-sfGFP
Plasmid#107420PurposeFor production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.DepositorInsertSpyCatcher
TagsHis tag, StrepII, and superfolder GFPExpressionBacterialPromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500
Plasmid#45788DepositorInsertdeGFP
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialPromoterpBAD-tetO1Available SinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBad-mKalama1
Plasmid#14892PurposeBacterial expression of mKalama1 blue fluorescent proteinDepositorInsertmKalama1
TagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…Available SinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
GST-p35
Plasmid#24895DepositorAvailable SinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
PARG-c002
Plasmid#173107PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-FnCas9GFP
Plasmid#130966Purposeexpression of FnCas9-GFP in bacterial cellsDepositorInsertHA-NLS-FnCas9
UseCRISPRTags6xHis-mEGFP-TEV-HA-NLSExpressionBacterialPromoterT7Available SinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only