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31,059 results

Showing: 3921 - 3960 of 31059 results

  2. pETM6-G6-vioABE-4A6-vioC

    Plasmid
    #73439
    Purpose
    Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PG6 and P4A6 (orthogonal T7-lac variants)
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73439-plasmid-map-sequence-id-135338

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  8. pBT1-proD-mCherry

    Plasmid
    #65823
    Purpose
    Const. mCherry expression , proD promoter, E. coli Expression
    Depositor
    Michael Lynch
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    proD E . coli Constitutive Promoter
    Available Since
    July 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65823-plasmid-map-sequence-id-111984

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  9. YARS_pNIC-CTB10H

    Plasmid
    #153059
    Purpose
    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    YARS (YARS1 Human)
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153059-plasmid-map-sequence-id-304479

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  20. pASKt15C+SrtA7

    Plasmid
    #64986
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64986-plasmid-map-sequence-id-109956

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  21. GST-thr-RAF1-RBD

    Plasmid
    #86033
    Purpose
    Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation
    Depositor
    Dominic Esposito
    Insert
    RAF1 RBD (Ras-binding domain) (RAF1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Contains residues 1-149
    Promoter
    T7
    Available Since
    Jan. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    86033-plasmid-map-sequence-id-170056

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  23. pNBU2_repA2

    Plasmid
    #211911
    Purpose
    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
    Depositor
    Adam Arkin
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211911-plasmid-map-sequence-id-421618

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  25. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184248-plasmid-map-sequence-id-370322

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  27. pcDNA3.1.Gα12-LgB115

    Plasmid
    #134363
    Purpose
    Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα12-LgB115 (GNA12 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134363-plasmid-map-sequence-id-306384

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  28. pAP114

    Plasmid
    #120799
    Purpose
    E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Insert
    Pxyl::mCherryOpt
    Expression
    Bacterial
    Mutation
    1.4 kb xylR-Pxyl DNA fragment from C. difficile R…
    Promoter
    Pxyl
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120799-plasmid-map-sequence-id-235314

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  30. pTP434

    Plasmid
    #104167
    Purpose
    Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling
    Depositor
    Dirk Görlich
    Article
    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554
    Insert
    Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
    Tags
    14x Histidine tag and NEDD8 from Brachypodium dis…
    Expression
    Bacterial
    Mutation
    3x Cysteines located at bps 493-495, 520-522, and…
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104167-plasmid-map-sequence-id-200061

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  35. pTriEx-mCherry-PA-Rac1-T17N

    Plasmid
    #22029
    Purpose
    Expression of photoactivatable Rac1 (dominant-negative mutant).
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22029-plasmid-map-sequence-id-239317

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  37. pRS413-GAL1-yEGFP1

    Plasmid
    #40235
    Depositor
    Michael Benton
    Insert
    yEGFP1
    Tags
    HIS3MX6
    Expression
    Bacterial
    Promoter
    GAL1
    Available Since
    Oct. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
    40235-plasmid-map-sequence-id-51356

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Showing: 3921 - 3960 of 31059 results