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80,562 results

Showing: 4521 - 4560 of 80562 results

  2. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  9. TMED10-bio-His

    Plasmid
    #51852
    Purpose
    Expresses full-length Transmembrane emp24 domain-containing protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TMED10 (TMED10 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51852-plasmid-map-sequence-id-79739

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  11. epi-BE4max-NG

    Plasmid
    #135977
    Purpose
    Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
    Depositor
    Yongming Wang
    Article
    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only

  16. TFORF0589

    Plasmid
    #142653
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXO1 (FOXO1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142653-plasmid-map-sequence-id-397779

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  21. pCAG-VSVN

    Plasmid
    #64087
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain nucleoprotein gene
    Depositor
    Ian Wickersham
    Insert
    VSV nucleoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64087-plasmid-map-sequence-id-436491

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  23. pAK_EF1a_CasRx_Hygro

    Plasmid
    #134840
    Purpose
    Lentiviral expression of CasRx T2A Hygromycin
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Hygromycin
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134840-plasmid-map-sequence-id-266452

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  26. pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE

    Plasmid
    #89609
    Purpose
    over-expression of VEGF-A(165) in human cells
    Depositor
    Fernando Fierro
    Insert
    VEGF-A(165) (VEGFA Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    K188E
    Promoter
    MNDU3
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    89609-plasmid-map-sequence-id-183289

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  27. pcDNA3.1.D2-NP

    Plasmid
    #134366
    Purpose
    Nanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    D2-NP (DRD2 Human)
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134366-plasmid-map-sequence-id-306391

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  28. pCAG-VSVP

    Plasmid
    #64088
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene
    Depositor
    Ian Wickersham
    Insert
    VSV phosphoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64088-plasmid-map-sequence-id-438903

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  33. pcDNA3.1-3'3xFlag

    Plasmid
    #208616
    Purpose
    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208616-plasmid-map-sequence-id-418312

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  36. TFORF2746

    Plasmid
    #144351
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HELZ2 (PRIC285 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144351-plasmid-map-sequence-id-404198

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Showing: 4521 - 4560 of 80562 results