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Mammalian Expression

75,820 results

Showing: 4521 - 4560 of 75820 results

  4. PB-CMV-MCS-Puro

    Plasmid
    #219794
    Purpose
    Mammalian expression plasmids using piggyBac Transposon System
    Depositor
    Keiichi Nakayama
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    219794-plasmid-map-sequence-id-441249

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  5. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Article
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    117686-plasmid-map-sequence-id-236350

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  10. pCS2+ GFP-2xESP3I-IRES-mCherry

    Plasmid
    #230996
    Purpose
    Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.
    Depositor
    Michael Rape
    Article
    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:
    Type
    Empty backbone
    Use
    Tags
    AcGFP1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    230996-plasmid-map-sequence-id-457945

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  12. pCMV5 TBRI-His

    Plasmid
    #19161
    Depositor
    Joan Massague
    Insert
    transforming growth factor, beta receptor 1 (TGFBR1 Human)
    Use
    Tags
    His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 1, 2008
    Availability
    Academic Institutions and Nonprofits only
    19161-plasmid-map-sequence-id-364368

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  18. pAPV-DsRed2-ER-5

    Plasmid
    #170570
    Purpose
    Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170570-plasmid-map-sequence-id-335779

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  25. pCMV-EGFP-p50

    Plasmid
    #107977
    Purpose
    Expresssion of NF-kB p50
    Depositor
    Georg Weber
    Insert
    NF-kB p50 (Nfkb1 Mouse)
    Use
    Tags
    N/A
    Expression
    Mammalian
    Mutation
    Amino acids VQACI added to C-terminus
    Promoter
    CMV
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107977-plasmid-map-sequence-id-218208

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  26. pDRM210 LGP (Luciferase GFP Puro)

    Plasmid
    #174722
    Purpose
    Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    eGFP
    pac
    Use
    Lentiviral and Luciferase
    Tags
    E2A linker and F2A linker
    Expression
    Mammalian
    Mutation
    Promoter
    MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    174722-plasmid-map-sequence-id-348963

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  27. mEmerald-Alpha-V-Integrin-25

    Plasmid
    #53985
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Alpha-V Integrin (ITGAV Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    53985-plasmid-map-sequence-id-257384

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  28. pENN.AAV.CAG.tdTomato.WPRE.SV40

    Plasmid
    #105554
    Purpose
    AAV expression of tdTomato from CAG promoter
    Depositor
    James M. Wilson
    Insert
    tdtomato
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105554-plasmid-map-sequence-id-202390

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  30. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122239-plasmid-map-sequence-id-292012

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  31. JD150

    Plasmid
    #229485
    Purpose
    Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, VA RNA I, E4orf6, L4-22k/33k
    Use
    AAV and Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    229485-plasmid-map-sequence-id-455002

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  33. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

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  36. pLenti CMV/TO GFP-MDC1 (779-2)

    Plasmid
    #26285
    Depositor
    Eric Campeau
    Insert
    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    26285-plasmid-map-sequence-id-228307

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Showing: 4521 - 4560 of 75820 results