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Mammalian Expression

76,289 results

Showing: 6051 - 6100 of 76289 results

  7. ORFtag_TetR-SD_Frame2

    Plasmid
    #220982
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220982-plasmid-map-sequence-id-437118

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  8. ORFtag_TetR-SD_Frame3

    Plasmid
    #220983
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220983-plasmid-map-sequence-id-437500

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  10. mRFP-Rab7

    Plasmid
    #14436
    Purpose
    Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.
    Depositor
    Ari Helenius
    Article
    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.
    Insert
    Rab 7 (RAB7A Canis familialis)
    Use
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    3 silent mutations near the C-terminus
    Promoter
    Available since
    March 23, 2007
    Availability
    Academic Institutions and Nonprofits only
    14436-plasmid-map-sequence-id-229535

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  12. mCherry-Vimentin-N-18

    Plasmid
    #55158
    Purpose
    Localization: Intermediate Filaments, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55158-plasmid-map-sequence-id-267740

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  14. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197064
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197064-plasmid-map-sequence-id-402605

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  28. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid
    #170378
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Article
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin promoter
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    170378-plasmid-map-sequence-id-333532

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  32. pSV40-mCherry-P2A-Hygro

    Plasmid
    #106478
    Purpose
    Expression of mCherry and Hygromycin resistance gene from SV40 promoter
    Depositor
    Alex Hewitt
    Inserts
    mCherry
    Hygromycin B resistance
    Use
    Tags
    Expression
    Mammalian and Plant
    Mutation
    Promoter
    SV40 promoter
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106478-plasmid-map-sequence-id-207986

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  35. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  39. pLentiCMV_HyPer7RgDAAO

    Plasmid
    #217653
    Purpose
    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane
    Depositor
    Seth Parker
    Article
    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi
    Insert
    HyPer7 D amino acid oxidase
    Use
    Lentiviral
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Promoter
    CMV
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217653-plasmid-map-sequence-id-441459

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  40. TFORF2971

    Plasmid
    #144447
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NR1H4 (NR1H4 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144447-plasmid-map-sequence-id-391596

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  42. PA-mCherry-Alpha-5-Integrin-12

    Plasmid
    #62790
    Purpose
    Localization: Focal Adhesions, Excitation:400 / 504, Emission:515 / 517
    Depositor
    Michael Davidson
    Insert
    Alpha5Integrin (ITGAV Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  46. CMV-LUC2CP/ARE

    Plasmid
    #62857
    Purpose
    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    destabilizing sequences (DS) added to the C termi…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62857-plasmid-map-sequence-id-212900

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  47. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

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Showing: 6051 - 6100 of 76289 results