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Mammalian Expression

76,423 results

Showing: 11651 - 11700 of 76423 results

  1. TFORF0574

    Plasmid
    #143714
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HOXA13 (HOXA13 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143714-plasmid-map-sequence-id-407845

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  8. GPR52-Tango

    Plasmid
    #66362
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR52 (GPR52 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pDAC569

    Plasmid
    #195241
    Purpose
    Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195241-plasmid-map-sequence-id-384464

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  22. TFORF2910

    Plasmid
    #143665
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIC (NFIC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143665-plasmid-map-sequence-id-400441

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  23. TFORF2912

    Plasmid
    #143667
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIC (NFIC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    143667-plasmid-map-sequence-id-353757

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  24. pREVtre GFP

    Plasmid
    #128069
    Purpose
    Retroviral tet inducible EGFP
    Depositor
    Oskar Laur
    Insert
    tet inducible EGFP
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    no
    Promoter
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128069-plasmid-map-sequence-id-255185

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  25. pmSTARRseq1

    Plasmid
    #96945
    Purpose
    CpG free plasmid for testing effects of methylation on regulatory activity
    Depositor
    Jenny Tung
    Type
    Empty backbone
    Use
    Mstarr-seq screening vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CpG -free EF1 promoter
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    96945-plasmid-map-sequence-id-191112

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  28. pLV V5-lamin B1

    Plasmid
    #175108
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb1
    Depositor
    Larry Gerace
    Insert
    Lmnb1 (Lmnb1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175108-plasmid-map-sequence-id-351795

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  34. pWZL-N1ICD-Flag

    Plasmid
    #171171
    Purpose
    Retroviral vector for expression of NOTCH1 intracellular domain with a C-terminal FLAG tag (to be used in conjunction with Phoenix packaging cells).
    Depositor
    Masashi Narita
    Article
    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do
    Insert
    NOTCH1 intracellular domain (NOTCH1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Will express residues 1758-2556 of human NOTCH1, …
    Promoter
    Viral LTR
    Available since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    171171-plasmid-map-sequence-id-338649

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  35. pSB-Bleo Gal4 tBFP

    Plasmid
    #139190
    Purpose
    Gal4-inducible tag blue fluorescent protein (tBFP) in pSB-Bleo Gal4 Sleeping Beauty backbone.
    Depositor
    Neal Devaraj
    Insert
    tBFP
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Gal4 CMV and STAT3
    Available since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    139190-plasmid-map-sequence-id-309397

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  36. pUBC-popTag-QtE-mScarlet

    Plasmid
    #240101
    Purpose
    Mammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.
    Depositor
    Liam Holt
    Article
    Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e
    Insert
    Encapsulin
    Use
    Lentiviral
    Tags
    mScarlet and popZ
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    UBC promoter
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    240101-plasmid-map-sequence-id-476613

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  40. gWiz_BM40-vAchT-Cytosolic-VL-hIgG1-CL

    Plasmid
    #218447
    Purpose
    mammalian expression plasmid for the light chain fragment of an anti-vAchT Fab domain binding to the cytosolic side of the transporter
    Depositor
    Axel Brunger
    Insert
    vAchT-Cytosolic-VL-hIgG1-CL
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    218447-plasmid-map-sequence-id-431100

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  41. gWiz_BM40-vGLUT1-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218438
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vGLUT1 Fab domain
    Depositor
    Axel Brunger
    Insert
    vGLUT1-Vh-hIgG1-CH2
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218438-plasmid-map-sequence-id-434501

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  42. gWiz_BM40-vAchT-Cytosolic-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218446
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vAchT Fab domain binding to the cytosolic side of the transporter
    Depositor
    Axel Brunger
    Insert
    vAchT-Cytosolic-Vh-hIgG1-CH6
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218446-plasmid-map-sequence-id-434499

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  43. gWiz_BM40-vGLUT1-VL-hIgG1-CL

    Plasmid
    #218439
    Purpose
    mammalian expression plasmid for the light chain fragment of an anti-vGLUT1 Fab domain
    Depositor
    Axel Brunger
    Insert
    vGLUT1-VL-hIgG1-CL
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218439-plasmid-map-sequence-id-431102

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  44. gWiz_BM40-vMAT2-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218442
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vMAT2 Fab domain
    Depositor
    Axel Brunger
    Insert
    vMAT2-Vh-hIgG1-CH4
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218442-plasmid-map-sequence-id-431084

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  45. gWiz_BM40-vMAT2-VL-hIgG1-CL

    Plasmid
    #218443
    Purpose
    mammalian expression plasmid for the light chain fragment of an anti-vMAT2 Fab domain
    Depositor
    Axel Brunger
    Insert
    vMAT2-VL-hIgG1-CL
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218443-plasmid-map-sequence-id-431083

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  46. MRGPRX1-DuET

    Plasmid
    #213344
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MRGPRX1 (MRGPRX1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213344-plasmid-map-sequence-id-430872

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  48. pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

    Plasmid
    #212961
    Purpose
    Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_backbone
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212961-plasmid-map-sequence-id-422482

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  50. DD-DamV133A-tdTomato-LMNB1

    Plasmid
    #159604
    Purpose
    Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.
    Depositor
    Aaron Streets
    Article
    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.
    Insert
    DD-DamV133A-tdTomato-LMNB1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Dam V133A mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159604-plasmid-map-sequence-id-311954

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Showing: 11651 - 11700 of 76423 results