78,292 results

1. pTriEx-mCherry-LOV2

   Plasmid

   #81041

   Purpose

   Mammalian or insect expression for wt LOV2

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV2
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV, p10

   Available Since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.10/RSV_SF3B3-Xon_Luciferase

   Plasmid

   #174659

   Purpose

   Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.

   Depositor

   Beverly L. Davidson

   Article

   Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

   Insert

   Xon cassette
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Kozak ATG initiation sequence in middle exon

   Promoter

   RSV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Sec31a #861#640

   Plasmid

   #42123

   Depositor

   Ayano Satoh

   Article

   Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

   Insert

   Sec31a (SEC31A Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCMV4 p65

   Plasmid

   #21966

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NFkB p65 subunit (RELA Human)
   

   Expression

   Mammalian

   Mutation

   This construct was the product of 3 subcloning pr…

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1+-Tornado-nLuc

   Plasmid

   #212611

   Purpose

   For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   Tornado-CVB3-nLuc
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-Flag-CtIP

   Plasmid

   #71108

   Purpose

   mammalian expession of Flag-CtlP

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Please see Depositor Comments

   Promoter

   TRE promoter, Tet ON

   Available Since

   Nov. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. MSCV IRES Luciferase

   Plasmid

   #18760

   Depositor

   Scott Lowe

   Type

   Empty backbone

   Use

   Luciferase and Retroviral

   Expression

   Mammalian

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. Twitch-2B pcDNA3

   Plasmid

   #49531

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-2B
   

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-3xFLAG-TERT

   Plasmid

   #51631

   Purpose

   Mammalian expression of 3xFLAG tagged TERT, N-terminal.

   Depositor

   Steven Artandi

   Article

   Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

   Insert

   TERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. mNeonGreen-APEX3

    Plasmid

    #202590

    Purpose

    Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

    Depositor

    Reuben Harris

    Article

    Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

    Insert

    mNeonGreen-APEX3-V5 (APX1 )
    

    Use

    Retroviral

    Tags

    V5 epitope and mNeonGreen

    Expression

    Mammalian

    Mutation

    APEX2 L242A

    Promoter

    MESV LTR

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

    Plasmid

    #129631

    Purpose

    Visualization of Akt and ERK activity

    Depositor

    Dorus Gadella

    Article

    Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

    Insert

    H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDisplay-GRAB_SST1.0

    Plasmid

    #208664

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW-Flag-CtIP-T847E

    Plasmid

    #71110

    Purpose

    mammalian expession of Flag-CtlP T847E mutation

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    CtlP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T847E

    Promoter

    TRE promoter, Tet ON

    Available Since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. CMV-Hylight-T152E

    Plasmid

    #193448

    Purpose

    A binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)

    Depositor

    Richard Goodman

    Article

    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Binding-Dead Hylight
    

    Expression

    Mammalian

    Mutation

    Threonine 67 (ACT) mutated to a Glutamic Acid (GA…

    Promoter

    CMV

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C1-hAtg2A

    Plasmid

    #36456

    Depositor

    Noboru Mizushima

    Article

    Velikkakath et al Mol Biol Cell. 2012 Mar;23(5):896-909. E

    Insert

    autophagy-related protein 2 homolog A (ATG2A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed Proline 656 to Arginine

    Promoter

    CMV

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS

    Plasmid

    #158741

    Purpose

    Expresses influenza A virus trimeric hemagglutinin A/H1/MI/15 fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

    Insert

    Hemagglutinin A/MI/45/15 H1N1
    

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    AA61-469

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. LRG2.1_Neo

    Plasmid

    #125593

    Purpose

    Lentiviral expression of sgRNA with GFP and neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

    Plasmid

    #164611

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Expression

    Mammalian

    Mutation

    S383T

    Promoter

    CMV

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LifeAct-mGarnet2

    Plasmid

    #104308

    Purpose

    codes for a fusion of fluorescent protein mGarnet2 and lifeact that targets F-actin in live cells

    Depositor

    Ulrich Nienhaus

    Article

    Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

    Insert

    LifeAct-mGarnet2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmEGFP-C1

    Plasmid

    #36412

    Depositor

    Benjamin Glick

    Type

    Empty backbone

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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