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Mammalian Expression

76,323 results

Showing: 281 - 320 of 76323 results

  1. pcDNA-Dlx6

    Plasmid
    #99480
    Purpose
    Mammalian expression of the Distal-less Homeobox 6 gene
    Depositor
    Jhumku Kohtz
    Insert
    Dlx6 (Dlx6 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    99480-plasmid-map-sequence-id-190484

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  3. SaCas9v3-Puro

    Plasmid
    #178813
    Purpose
    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178813-plasmid-map-sequence-id-354841

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  5. tdEos-Rad21-N-18

    Plasmid
    #57666
    Purpose
    Localization: Centromere, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Rad21 (RAD21 Human)
    Use
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57666-plasmid-map-sequence-id-233120

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  7. pGBW-m4134902

    Plasmid
    #151912
    Purpose
    Mammalian expression plasmid for Human coronavirus 229E nucleocapsid
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    Human coronavirus 229E nucleocapsid protein (N Human coronavirus 229E)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    151912-plasmid-map-sequence-id-307617

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  8. GPR27-Tango

    Plasmid
    #66349
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR27 (GPR27 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66349-plasmid-map-sequence-id-456518

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  10. pGBW-m4134908

    Plasmid
    #151957
    Purpose
    Mammalian expression plasmid for Human coronavirus NL63 nucleocapsid
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    Human coronavirus NL63 nucleocapsid protein (HCNV63gp6 Human coronavirus NL63)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    151957-plasmid-map-sequence-id-306275

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  11. pLXSN p110 CUX1

    Plasmid
    #90471
    Purpose
    Retroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectively
    Depositor
    Alain Nepveu
    Article
    Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11
    Insert
    CUX1 (amino acids 747-1505) (CUX1 Human)
    Use
    Retroviral
    Tags
    HA and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Moloney murine leukemia virus long terminal repeat
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    90471-plasmid-map-sequence-id-205963

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  15. mCherry-LC-Myosin-N-7

    Plasmid
    #55072
    Purpose
    Localization: Light Chain Myosin, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    LC-Myosin
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pcDNA3-Akt-STOPS

    Plasmid
    #175006
    Purpose
    Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.
    Depositor
    Jin Zhang
    Article
    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:
    Insert
    mCherry-Akt-STOPS
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    175006-plasmid-map-sequence-id-344970

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  17. pCDNA3-hTICAM1-VenusFlag

    Plasmid
    #197632
    Purpose
    Human TRIF protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TICAM1 (TICAM1 Human)
    Use
    Tags
    Venus-Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197632-plasmid-map-sequence-id-392629

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  24. pTRE-T2-∆mIR-IRES-Hygromycin

    Plasmid
    #105169
    Purpose
    Mammalian expression vector for expressing tandem cDNAs
    Depositor
    Michael E. Wright
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet/CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    105169-plasmid-map-sequence-id-204231

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  31. pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)

    Plasmid
    #223087
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223087-plasmid-map-sequence-id-447656

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  32. A24M04 Ig kappa light chain-pFEEKW

    Plasmid
    #234810
    Purpose
    Express Ig kappa light chain containing the VL region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1
    Depositor
    Susan Pierce
    Article
    Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12
    Insert
    A24M04 Igkappa light chain (IGKC, IGK Human)
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EEK promoter
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    234810-plasmid-map-sequence-id-466812

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  38. SaCas9 GFP V3

    Plasmid
    #226962
    Purpose
    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226962-plasmid-map-sequence-id-450821

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  39. pCAG-EGFP-C-IFT172

    Plasmid
    #218734
    Purpose
    Expresses N-terminally EGFP-tagged IFT172 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    IFT172 (IFT172 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218734-plasmid-map-sequence-id-434222

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  40. Kv1.4 K+ channel (extracellular) scFv [L71/5]

    Plasmid
    #190517
    Purpose
    Mammalian Expression of Kv1.4 K+ channel (extracellular) scFV. Derived from hybridoma L71/5.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Kv1.4 K+ channel (extracellular) (Rattus norvegicus) recombinant scFV (Kcna4 Mouse)
    Use
    Tags
    HA, Sortase, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    190517-plasmid-map-sequence-id-377123

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Showing: 281 - 320 of 76323 results