1,748 results

1. pJW1659

   Plasmid

   #154314

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::AID*::3xFLAG for NT slot
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. T444T-GFP(NCO)

   Plasmid

   #183972

   Purpose

   Enhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFP

   Depositor

   David Matus

   Article

   Palmisano et al CourseSource, 2022

   Insert

   GFP (non-codon-optimized)
   

   Use

   RNAi

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCFJ2369 - intron GG1 - 250bp

   Plasmid

   #159877

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG1 - 250bp
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDD287 (mKate2^SEC^3xMyc

   Plasmid

   #70685

   Purpose

   mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   mKate2-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xMyc and C. elegans codon-optimized mKate2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJW1821

   Plasmid

   #163092

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   mScarlet^SEC (Lox511I)^::3xMyc
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJW1816

   Plasmid

   #154320

   Purpose

   Multi-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBN1

   Plasmid

   #86710

   Purpose

   C. elegans injection marker

   Depositor

   Peter Askjaer

   Article

   Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

   Insert

   Plmn-1::mCherry::his-58::pie-1 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBN42

   Plasmid

   #86713

   Purpose

   C. elegans injection marker

   Depositor

   Peter Askjaer

   Article

   Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

   Insert

   Pmyo-3::GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEY14

   Plasmid

   #191059

   Purpose

   acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   acr-5(orf) (acr-5 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmec-4::Chrimson::SL2::mCherry::unc-54

    Plasmid

    #107745

    Purpose

    Express Chrimson and mCherry in mechanosensory neurons.

    Depositor

    Andrew Leifer

    Article

    Liu et al Elife. 2018 Jun 26;7. pii: 36419. doi: 1

    Inserts

    Chrimson
    mCherry
    

    Use

    Tags

    Expression

    Insect and Worm

    Mutation

    Promoter

    mec-4

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159857

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHIP_RNA2

    Plasmid

    #61260

    Purpose

    Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    heat shock promoter

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

    Plasmid

    #159817

    Purpose

    Positive control for germline GFP expression from simple array in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid

    #70684

    Purpose

    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.

    Depositor

    Bob Goldstein

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAS1-Prig-3::tagBFP

    Plasmid

    #165598

    Purpose

    rig-3 promoter derive BFP expression in AVA head neurons.

    Depositor

    Andrew Leifer

    Article

    Hallinen et al Elife. 2021 Jul 29;10:e66135. doi: 10.75

    Insert

    Prig-3, tagBFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

    Plasmid

    #159808

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159845

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJH1371

    Plasmid

    #199631

    Purpose

    Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    GPI
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    Pregf-1

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pSM-GFPnovo2

    Plasmid

    #116943

    Purpose

    GFPnovo2_unc-54 utr vector for expression in C. elegans

    Depositor

    Kota Mizumoto

    Article

    Hendi et al MicroPubl Biol. 2018 Sep 6;2018:10.17912

    Insert

    C. elegans codon optimized GFPnovo2
    

    Use

    Tags

    GFPnovo2

    Expression

    Worm

    Mutation

    Y145F, V163A, S202T, L221V

    Promoter

    none

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEM238

    Plasmid

    #161515

    Purpose

    Neuronal expression of histamine-sensitive chloride channel for negative selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

    Insert

    pEXP[Psnt-1 | histamine | rpl-3 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Psnt-1

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLT61

    Plasmid

    #1690

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    RNAi

    Tags

    T7p, unc224616, unc2218148, unc2218082, unc223827…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Pmec-18 caspase-3 (p12)::nz [TU#813]

    Plasmid

    #16082

    Depositor

    Martin Chalfie

    Article

    Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

    Insert

    caspase-3 (p12) :: nz (CASP3 Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

    Plasmid

    #193048

    Purpose

    Heatshock expressed Cas9

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    hsp16.41p

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2

    Plasmid

    #182357

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Peft-3

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNMSB84

    Plasmid

    #199309

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…

    Promoter

    flp-18

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

    Plasmid

    #193049

    Purpose

    Encodes G-418 resistance

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    prsp-27::NeoR::unc-54 3’ UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    prsp-27

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSEM237 - pEXP[Prpl-3 | histamine | rpl-3 UTR]

    Plasmid

    #159796

    Purpose

    Histamine based negative selection marker to paralyze animals with arrays

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

    Insert

    pEXP[Prpl-3 | histamine | rpl-3 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCFJ1137

    Plasmid

    #84827

    Purpose

    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized GFP
    

    Use

    Tags

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Peft-3

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSF11-wElectra1

    Plasmid

    #184932

    Purpose

    Codon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmid

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    tag-168

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

    Plasmid

    #168173

    Purpose

    Expression of BeCyclOp in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

    Insert

    BeCyclOp (codon optimized for C. elegans)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge