Skip to main content
Addgene
Showing: 301 - 350 of 1735 results

  6. Ccas9LacZ

    Plasmid
    #113083
    Purpose
    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113083-plasmid-map-sequence-id-218959

    Enlarge

  7. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid
    #154840
    Purpose
    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.
    Depositor
    Patrick Phillips
    Article
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Worm
    Mutation
    HYGR∆ encodes aa 1-226
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154840-plasmid-map-sequence-id-307910

    Enlarge

  12. pJJR81

    Plasmid
    #75029
    Purpose
    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Mike Boxem
    Insert
    tagBFP2^SEC^3xFlag
    Use
    CRISPR
    Tags
    3xFLAG and C. elegans codon optimized tagBFP2
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    75029-plasmid-map-sequence-id-141917

    Enlarge

  35. pMB70

    Plasmid
    #47943
    Purpose
    Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences
    Depositor
    Mike Boxem
    Article
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    guide RNA
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Oct. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    47943-plasmid-map-sequence-id-68789

    Enlarge

  37. pCFJ1415

    Plasmid
    #84828
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns
    Depositor
    Andrew Fire
    Article
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Use
    Tags
    Expression
    Bacterial and Worm
    Mutation
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84828-plasmid-map-sequence-id-163670

    Enlarge

  38. pJH3628

    Plasmid
    #191445
    Purpose
    Prgef-1-Chrimson::UrSL2::wCherry unc-54 3' UTR C.elegans panneural expression of Chrimson RFP
    Depositor
    Mei Zhen
    Article
    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e
    Insert
    Chrimson
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Pregf-1
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191445-plasmid-map-sequence-id-377567

    Enlarge

Showing: 301 - 350 of 1735 results