Skip to main content
Addgene
Showing: 401 - 440 of 7933 results

  1. pHS3

    Plasmid
    #81038
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    NATMX - HO endonuclease
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. pAV116

    Plasmid
    #63183
    Purpose
    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63183-plasmid-map-sequence-id-104859

    Enlarge

  6. pHflu4

    Plasmid
    #203882
    Purpose
    pHI1 with insertion of monomeric red fluorescent protein (mRFP)
    Depositor
    Bogumil Karas
    Insert
    monomeric red fluorescent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203882-plasmid-map-sequence-id-403835

    Enlarge

  9. FRP2008

    Plasmid
    #159312
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mKOkappa under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    V2del
    Promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159312-plasmid-map-sequence-id-311212

    Enlarge

  10. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

    Enlarge

  14. FRP2370

    Plasmid
    #127576
    Purpose
    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection marker
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1
    Insert
    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    127576-plasmid-map-sequence-id-252228

    Enlarge

  24. pH3mCHSH2

    Plasmid
    #101053
    Purpose
    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.
    Depositor
    Jennifer Lodge
    Article
    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108
    Insert
    Cryptococcus mCherry expression plasmid
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Cryptococcus Histone3 promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    101053-plasmid-map-sequence-id-192942

    Enlarge

  26. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Article
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196040-plasmid-map-sequence-id-386150

    Enlarge

  27. pAV126

    Plasmid
    #63192
    Purpose
    pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    63192-plasmid-map-sequence-id-104870

    Enlarge

  29. FRP2365

    Plasmid
    #127575
    Purpose
    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection marker
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1
    Insert
    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    127575-plasmid-map-sequence-id-252224

    Enlarge

  31. pAW212

    Plasmid
    #172196
    Purpose
    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites
    Depositor
    Chang Liu
    Article
    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038
    Insert
    p10B2-AT110
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    10B2 (P1 specific promoter)
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    172196-plasmid-map-sequence-id-338565

    Enlarge

  32. pPICZc-ScAct1*-thymosinB-8His

    Plasmid
    #111148
    Purpose
    Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37
    Depositor
    Mohan Balasubramanian
    Article
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    Act1 (ACT1 Budding Yeast)
    Use
    Pichia pastoris integration plasmid
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Mutation
    Promoter
    AOX1
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111148-plasmid-map-sequence-id-214055

    Enlarge

  37. pJYDN

    Plasmid
    #162450
    Purpose
    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162450-plasmid-map-sequence-id-321442

    Enlarge

  40. pAG426-GAL-Alpha-synuclein

    Plasmid
    #181715
    Purpose
    Galactose inducible expression of Alpha-synuclein
    Depositor
    Alejandro Chavez
    Insert
    Alpha-synuclein (SNCA Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    181715-plasmid-map-sequence-id-436188

    Enlarge

Showing: 401 - 440 of 7933 results