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8,265 results

Showing: 651 - 700 of 8265 results
  1. pFA6a-mNeonGreen-KanMX6

    Plasmid
    #129099
    Purpose
    For tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen
    Depositor
    Insert
    mNeonGreen
    Expression
    Yeast
    Available Since
    July 31, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pRS410

    Plasmid
    #11258
    Depositor
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Feb. 24, 2006
    Availability
    Academic Institutions and Nonprofits only
  3. pTPGI_dCas9

    Plasmid
    #64279
    Purpose
    encodes yeast-optimized dCas9 synthetic transcription factor
    Depositor
    Insert
    Yeast-optimized dCas9
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    D10A & H840A relative to S. pyogenes Cas9
    Promoter
    pTPGI
    Available Since
    May 5, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pNA0304

    Plasmid
    #165612
    Purpose
    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)
    Depositor
    Insert
    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    NotI site replaced the CAN1 spacer in parent vect…
    Promoter
    SNR52
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pHCA/GAL4(1-93).ER.VP16

    Plasmid
    #108216
    Purpose
    Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain
    Depositor
    Insert
    Gal4 DBD-ERalpha HBD-VP16
    Expression
    Yeast
    Mutation
    ERalpha HBD has G400V mutation
    Promoter
    ADH1
    Available Since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pGR420

    Plasmid
    #213782
    Purpose
    Integrates onto p1 a partial LEU2 sequence to be subsequently used as an integration flank
    Depositor
    Insert
    MET15
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pCAS9c

    Plasmid
    #141250
    Purpose
    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pHS2

    Plasmid
    #81037
    Purpose
    HO endonuclease expression
    Depositor
    Insert
    HYGMX
    Expression
    Yeast
    Promoter
    TEF
    Available Since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
  9. pUDP004

    Plasmid
    #101165
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    ScTDH3
    Available Since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. pGR475

    Plasmid
    #213784
    Purpose
    Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker
    Depositor
    Insert
    WT TP-DNAP1 (recoded)
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. ZJOM31

    Plasmid
    #133645
    Purpose
    Integration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.
    Depositor
    Insert
    p1k-TGL3-GFP (TGL3 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. ZJOM64

    Plasmid
    #133655
    Purpose
    Integration of COX4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.
    Depositor
    Insert
    p1k-COX4-DuDre (COX4 Budding Yeast)
    Tags
    DuDre
    Expression
    Yeast
    Available Since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pDEST-N2H-F2-empty vector

    Plasmid
    #125552
    Purpose
    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)
    Depositor
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 651 - 700 of 8265 results