8,350 results
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Plasmid#105077PurposeYeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloningDepositorTypeEmpty backboneTagsNubGExpressionYeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only
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pLyGo-Kp-1
Plasmid#163143PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastPromoterAOX1Available SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
Plasmid#198417PurposeExpresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeastDepositorInsertOsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
ExpressionYeastPromoterADH1 promoterAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS316-IVa
Plasmid#31456DepositorInsertlinker
ExpressionYeastAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pXylP-HygR
Plasmid#168131PurposeExpression of HygR from the xylose-inducible xylP promoterDepositorInsertXylP-HygR
UseAspergillus expressionExpressionYeastPromoterxylPAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG416-Gal-PR50
Plasmid#84901Purposeinduce expression of dipeptide repeat PR in yeastDepositorAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Ptdh3-CAD-Ttdh1
Plasmid#127727PurposeYeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPtdh3Available SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAP05
Plasmid#102920PurposeDirect cloning and broad-host-range heterologous expression vectorDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHRW32
Plasmid#130972PurposeHyPr plasmid with ble markerDepositorInsertempty backbone
ExpressionYeastAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFS554
Plasmid#242854PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW3 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW3 promoterAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS553
Plasmid#242853PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW2 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW2 promoterAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTEF:ATP
Plasmid#92179PurposeHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.DepositorInsertATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
UseIntegration and expression into yeast.TagsYeast codon optimized mseCFP (donor) and cp173-mV…ExpressionYeastPromoterTEF1pAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lact-C2-GFP-p416
Plasmid#22853DepositorInsertLactadherin-C2
TagsGFPExpressionYeastAvailable SinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_XPR2
Plasmid#84609PurposeIntroduces DSB at XPR2 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gal:mCherry-uTEV3
Plasmid#135453PurposeYeast expression of uTEV3 fused to mCherryDepositorInsertmCherry-uTEv3
TagsmCherryExpressionYeastMutationS219V mutation improves stability , I138T/S153N/T…PromoterGalAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMetOYC-Dest
Plasmid#105082PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloningDepositorTypeEmpty backboneTagsCub-PLV and mOST4ExpressionYeastAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_AXP
Plasmid#84608PurposeIntroduces DSB at AXP locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJT11
Plasmid#98308PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3ClLIS1>TRPL41B
ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-V5-hphMX4
Plasmid#20779DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJH001
Plasmid#67641PurposeHigh copy Cas9 expression vector with LEU2 marker for yeast transformationDepositorInsertsCas9
Empty
UseCRISPRExpressionYeastAvailable SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVT36b
Plasmid#184437PurposeCRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p416 103Q GAL
Plasmid#1186DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAV0757
Plasmid#133509PurposeExpression of 3GFP tagged CRIB domain to monitor active Cdc42DepositorInsertpHis5(StuI)-p(pak1)-CRIB(gic2aa2-181)-3GFP-terminator(ScADH1)-kanMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p413 TEF NAPstar3
Plasmid#232740PurposeExpresses NADPH/NADP+ biosensor NAPstar3 in S. cerevisiae.DepositorInsertNAPstar3
ExpressionYeastAvailable SinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMVS102_P3-GFP-NATMX6
Plasmid#99048PurposeEGFP reporter with six binding sites for the Zif268 DNA binding domainDepositorInsertsMcIsaac 2014 P3 promoter
eGFP
clonNAT resistance
ExpressionYeastMutationGAL1 promoter with 4 GAL4 sites removed and repla…PromoterMcIsaac 2014 P3 promoterAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMZ379
Plasmid#74215PurposeCombined sgRNA/Cas9 vector with NatMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXNgate21-3HA
Plasmid#105078PurposeYeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloningDepositorTypeEmpty backboneTagsNubGExpressionYeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV0765
Plasmid#133501PurposeExpression of mCherry targeted to the nucleus with one NLS signalsDepositorInsertpLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX
ExpressionYeastAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJJB1402
Plasmid#218616PurposeExpress Pex15, Pex22, Pex4, Pex1, and Pex6DepositorInsertPex15
ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG413GAL-ccdB-HA
Plasmid#14237DepositorTypeEmpty backboneUseGateway CloningTags3HAExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPICZaa-STE2-cohis
Plasmid#133433PurposeYeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.DepositorInsertSTE2 (STE2 Budding Yeast)
ExpressionYeastMutationC59S/C252A, Codon optimized for P. pastoris, and …Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-L
Plasmid#51226Purposebi-directional expression vector for yeast, constitutively active, LEU2 selectionDepositorTypeEmpty backboneExpressionYeastPromoterGPD/ADH1Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB3045(gRNA XI-3)
Plasmid#73287PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK425 (GAL-OsTIR1(F74A))
Plasmid#140657PurposeGAL-OsTIR1(F74A)DepositorInsertGAL-OsTIR1(F74A)
ExpressionYeastAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yEmCFP-Hygro
Plasmid#44644DepositorInsertyEmCFP
ExpressionYeastMutationA206RAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYES2-gRNA-hyg-MCS
Plasmid#107734PurposeE. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.DepositorInsertX-3 site gRNA expression cassettes
UseCRISPRExpressionYeastPromoterSNR52pAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pV1393
Plasmid#111430PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK369
Plasmid#73594PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitopeDepositorInsertdelta-12 fatty acid acetylenase
TagsFLAG tagExpressionYeastPromoterGAL10Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
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ymScarlet
Plasmid#111917Purposeyeast optimized mScarletDepositorInsertymScarlet
ExpressionYeastPromoterGPDAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV0661
Plasmid#133476PurposeExpression in fission yeast from promoter pact1, integrated at ade6 locusDepositorInsertpAde6(PmeI)-p(act1)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymTurquoise2
Plasmid#118453PurposeOverexpression of yeast codon-optimized mTq2DepositorInsertymTurquoise2
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT2
Plasmid#98306PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-EGFP-ccdB
Plasmid#14323DepositorTypeEmpty backboneUseGateway CloningTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymNeongreen
Plasmid#193959PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeastDepositorInsertymNeonGreen
ExpressionYeastPromoterTEF1Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
yo-msGFP2
Plasmid#160471PurposeEncodes a yeast codon optimized version of msGFP2DepositorInsertyo-msGFP2
ExpressionYeastAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FLAG-APEX2-NES-HIS3
Plasmid#229230PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneExpressionYeastAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMG13
Plasmid#122932PurposeExpression of the regulator tTA.DepositorInserttTA
ExpressionYeastPromoterPcln3Available SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDP0437
Plasmid#221127PurposePlasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMXDepositorInsertpHluorin
ExpressionYeastAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRL_GI_PCB28
Plasmid#98745PurposeFor phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.DepositorInsertsUseSynthetic BiologyExpressionYeastMutationCodon optimized for Saccharomyces cerevisiaePromoterADH1m and PGK1Available SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only