8,350 results
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Plasmid#122060Purposegalactose inducible expression of GFP in yeast. marked with URA3DepositorInsertgreen fluorescent protein
ExpressionYeastPromoterGAL1Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-HALO-NAT
Plasmid#188931PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-HALO-NAT
ExpressionYeastAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB2195
Plasmid#67548PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCE41
Plasmid#174433PurposeC. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.DepositorInsertHYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
UseCRISPRExpressionBacterial and YeastAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBHM2404
Plasmid#173441PurposepRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-NAT1
TagsCBP-2xFLAGExpressionBacterial and YeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS306-PHIS3-GFP-μNS
Plasmid#116935PurposeYeast integrative vector for the expression of μNSDepositorInsertμNS
TagsGFPExpressionYeastPromoterHIS3Available SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC5
Plasmid#188602PurposeSuperior Conjugative Plasmid to Diverse YeastDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFS255
Plasmid#12382DepositorInsertthymidine kinase
ExpressionYeastAvailable SinceSept. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-F1-empty vector
Plasmid#125551PurposeN2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 alone without any attached prot…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#102951PurposeExpresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoterDepositorInsertAPEX2-NES
TagsFLAGExpressionYeastPromoterGAL1Available SinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSII41K
Plasmid#194522PurposeLow copy vector backbone with G418 selectivity, contains MCSDepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXRA2
Plasmid#63144PurposeADE2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxExpressionBacterial and YeastAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ube2v2
Plasmid#31430DepositorAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
HP-Cas9hGem-Ade2-LEU2-LINEAR
Plasmid#174839PurposepRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorphaDepositorInsertHH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
UseCRISPR and Synthetic BiologyExpressionBacterial and YeastPromoterTDH3pAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKL08
Plasmid#104432PurposepHuji in yeast expression vectorDepositorInsertpHuji
ExpressionYeastPromoterTef1Available SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIV515
Plasmid#177703PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAV0133
Plasmid#133467PurposeIntegration into fission yeast genome at ura4 locusDepositorTypeEmpty backboneExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH761-CEN-mCherry_v4
Plasmid#40599DepositorInsertmCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
ExpressionYeastMutationmCherry ORF partially codon optimised for yeast e…PromoterGPD (TDH3)Available SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS712
Plasmid#213041PurposeCRISPR activation based overexpression in Nakaseomyces (Candida) glabrataDepositorTypeEmpty backboneExpressionYeastAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pG4
Plasmid#162605PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
ExpressionYeastAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYDS2.0_AT118-L
Plasmid#222847PurposeYeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivityDepositorInsertAT118-L
ExpressionYeastAvailable SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB4906
Plasmid#106150PurposeEasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistanceDepositorInsertCas9
ExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG303-Gal-PA50
Plasmid#84906Purposeinduce expression of dipeptide repeat PA in yeastDepositorAvailable SinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYZ183
Plasmid#98425PurposePombe Expression Vector - nmt41 promoter with empty BsaI-pad - no markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ184
Plasmid#98426PurposePombe Expression Vector - nmt81 promoter with empty BsaI-pad - no markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-EGFP-ccdB
Plasmid#14316DepositorTypeEmpty backboneUseGateway CloningTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0 Tp
Plasmid#188619PurposeMutated pTA-Mob 2.0 Backbone in TraJ Promoter RegionDepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidExpressionBacterial and YeastAvailable SinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pV1326
Plasmid#111435PurposeS. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP8H
Plasmid#188062PurposePROSS-designed versatile peroxidase 8HDepositorInsertPROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
TagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation38 PROSS mutations, described in publicationAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pROS10
Plasmid#107924PurposeURA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pfa6a PhoCl-TsCC(B)::KANMX
Plasmid#185906PurposePlasmid for cloning PhoCl fused to TsCC(B) into yeast.DepositorInsertPhoCl-TsCC(B)
TagsPhoCl fluorophore with C-terminal TsCC(B)ExpressionYeastPromoterSP6Available SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM0641
Plasmid#129006PurposeIntegration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRR-AR
Plasmid#73045PurposeConstitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)DepositorInsertAndrogen receptor (AR Human)
ExpressionYeastMutationplease see depositor comments belowPromoterglyceraldehyde phosphate dehydrogenase (GPD)Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJH2971
Plasmid#100955PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSII42H-TDH3pr
Plasmid#194533PurposeHigh copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)DepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT166_GalL_A3A-BE3
Plasmid#145116PurposeExpressing base editor A3A-BE3 in yeast cellsDepositorInsertA3A-BE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS42H_PTEF1_dCas9_TCYC1
Plasmid#177155PurposeExpresses dCas9 in yeast cellsDepositorInsertNuclease-null Cas9
TagsSV40 NLSExpressionYeastPromoterTEF1 promoterAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS316-4M5.3
Plasmid#45831DepositorInsert4M5.3
TagsFLAGExpressionYeastPromoterGAL1Available SinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L
Plasmid#89576PurposecaCas9 integrating vector into the Neut5L locusDepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-NLS-3HA-KanMX6
Plasmid#228022PurposeTo integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastAvailable SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-NLS-3HA-HISMX6
Plasmid#228027PurposeTo integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with HISMX6 markerDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-NLS-3HA-KanMX6
Plasmid#228026PurposeTo integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
426Gal-FUS-YFP
Plasmid#29592DepositorAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH3
Plasmid#83553PurposeUsed to evaluate the expression output of HXT7 promoter with yEGFP used as the reporter gene.DepositorInsertHXT7 promoter-yEGFP
ExpressionYeastPromoterHXT7Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRR-ERalpha-5Z
Plasmid#23061DepositorInsertERalpha (ESR1 Human)
ExpressionYeastAvailable SinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pE6n
Plasmid#17460Purposemodified pENTR vector for N-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBHM2406
Plasmid#173442PurposepRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-HYGB
TagsCBP-2xFLAGExpressionBacterial and YeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
YEp195-CUP1-His-Ubi
Plasmid#99540PurposeYeast episomal vector for expression of His-tagged ubiquitin; URA3 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only