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Yeast Expression

7,933 results

Showing: 901 - 950 of 7933 results

  1. pYeDP60-hATP13A2-WT

    Plasmid
    #171377
    Purpose
    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888
    Depositor
    Peter Vangheluwe
    Article
    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:
    Insert
    human ATP13A2 variant 2 wild-type
    Use
    Tags
    BAD tag
    Expression
    Yeast
    Mutation
    Promoter
    URA3
    Available since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171377-plasmid-map-sequence-id-342929

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  10. pV1200

    Plasmid
    #111429
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111429-plasmid-map-sequence-id-212464

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  11. pACT2-μ3A

    Plasmid
    #198174
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-3 μ3A
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    silent substitution in codon 225
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198174-plasmid-map-sequence-id-387537

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  12. pACT2-μ3B

    Plasmid
    #198175
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-3 μ3B
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198175-plasmid-map-sequence-id-387536

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  20. pCfB2225

    Plasmid
    #67553
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67553-plasmid-map-sequence-id-116384

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  23. pFA6a-link-tdTomato-SpHis5

    Plasmid
    #44640
    Purpose
    Yeast tagging vector to create gene fusion with tdTomato with SpHis5 selection
    Depositor
    Kurt Thorn
    Insert
    tdTomato
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44640-plasmid-map-sequence-id-61108

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  24. pRS267

    Plasmid
    #122380
    Purpose
    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon
    Depositor
    Rebecca Shapiro
    Article
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Insert
    HSP90
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122380-plasmid-map-sequence-id-243382

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  26. pFA6a-link-yEmCFP-Hygro

    Plasmid
    #44644
    Depositor
    Kurt Thorn
    Insert
    yEmCFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    A206R
    Promoter
    Available since
    May 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    44644-plasmid-map-sequence-id-61113

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  36. pILGFP5EC4

    Plasmid
    #185880
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185880-plasmid-map-sequence-id-370826

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  37. pILGFP3AG4ARSd

    Plasmid
    #185876
    Purpose
    A plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185876-plasmid-map-sequence-id-370819

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  38. pRS316-RGR-GFP

    Plasmid
    #51056
    Purpose
    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    RGR-GFP
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Yeast ADH1 promoter
    Available since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51056-plasmid-map-sequence-id-77326

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Showing: 901 - 950 of 7933 results