162,619 results

1. SPYtag-Histag-Avidin

   Plasmid

   #214836

   Purpose

   This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   SPYtag-Histag-Avidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-tMCK-ER-TurboID

   Plasmid

   #149412

   Purpose

   Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Insert

   ER-TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mutation

   Promoter

   tMCK

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HPRT2b-pGEM

   Plasmid

   #69539

   Purpose

   gRNA targeting human HPRT gene

   Depositor

   Hong Yan

   Article

   Liao et al Nucleic Acids Res. 2015 Jun 29. pii: gkv

   Insert

   HPRT gRNA2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLO87

   Plasmid

   #91726

   Purpose

   mycobacterial expression of mCherry from Hsp60 promoter

   Depositor

   Graham Hatfull

   Article

   Oldfield et al J Bacteriol. 2014 Oct;196(20):3589-97. d

   Insert

   hsp60 promoter
   

   Use

   Mycobacterial expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   hsp60

   Available since

   May 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-Sp-pegRNA-HEK3_CTT_ins

   Plasmid

   #132778

   Purpose

   S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   HEK3_CTT_ins pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMU-PERr

   Plasmid

   #61202

   Purpose

   Fluorescent reporter for peroxisomes mCherry-SKL expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   mCherry-SKL
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CAG.Ruby2sm-Flag.WPRE.SV40

   Plasmid

   #98925

   Purpose

   AAV expression of Ruby2 spaghetti monster FLAG construct

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   Ruby2-smFP-Flag
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBA

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pv9_TAF3_2xPHD-13XL-BASU

   Plasmid

   #179414

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3.2xPHD-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   TAF3.2xPHD-13XL-BASU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV-U6+27-Tornado-RhoBAST

   Plasmid

   #196341

   Purpose

   Overexpression of circular RhoBAST

   Depositor

   Murat Sunbul

   Article

   Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

   Insert

   Tornado-RhoBAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CAG-Cas9-T2A-EGFP-ires-puro

    Plasmid

    #78311

    Purpose

    Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

    Depositor

    Timo Otonkoski

    Article

    Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

    Insert

    WT SpCas9-T2A-EGFP-ires-puromycin resistance
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xHA-TurboID pRetrox

    Plasmid

    #155203

    Purpose

    For doxycycline-inducible expression of 3xHA-TurboID on N-terminus of bait protein

    Depositor

    Kyle Roux

    Article

    May et al Cells. 2020 Apr 25;9(5). pii: cells90510

    Insert

    3xHA-TurboID
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promotor

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_BiPVe4_eGFP

    Plasmid

    #221463

    Purpose

    AAV construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFP

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3 IRF9-STAT2C

    Plasmid

    #37544

    Depositor

    Curt Horvath

    Article

    Kraus et al J Biol Chem. 2003 Apr 11;278(15):13033-8

    Insert

    IRF-9 STAT2 C (IRF9, STAT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. SPHK2 gRNA (BRDN0001148465)

    Plasmid

    #77093

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-NFAT1(4-460)-GFP

    Plasmid

    #11107

    Purpose

    Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

    Depositor

    Anjana Rao

    Article

    Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Tags

    3x HA and GFP

    Expression

    Mammalian

    Mutation

    Contains amino acids 4-460 of NFAT1; L78P compare…

    Promoter

    CMV

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRANS_220

    Plasmid

    #91113

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-CAG-FLEX-BFP2-(mCherry)’

    Plasmid

    #115234

    Purpose

    Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    mTagBFP2 and mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG

    Plasmid

    #129632

    Purpose

    Visualization of Akt and ERK activity

    Depositor

    Dorus Gadella

    Article

    Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

    Insert

    PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAG-LiLac

    Plasmid

    #184570

    Purpose

    Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

    Depositor

    Gary Yellen

    Article

    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

    Insert

    LiLac fluorescent sensor of lactate
    

    Use

    AAV

    Tags

    His7

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #161928

    Purpose

    Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    Surface marker and Citrine activation reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLKO.1 mCherry

    Plasmid

    #128073

    Purpose

    puro exchanged to mCherry

    Depositor

    Oskar Laur

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    no

    Promoter

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDR-GW FLIPglu-600μΔ13V

    Plasmid

    #28001

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    FLIPglu-600μΔ13V
    

    Use

    Tags

    eCFP and eYFP

    Expression

    Yeast

    Mutation

    F16A in mglB

    Promoter

    Available since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

23. pGL3-rev-erbα

    Plasmid

    #186823

    Purpose

    Fluorescent reporter for rev-erbα expression

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    rev-erbα promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    rev-erbα promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pD649-HAsp-SIGLEC7-COMP5AP-AviTag-9xHis

    Plasmid

    #157337

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SIGLEC7 (SIGLEC7 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF0757

    Plasmid

    #143074

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NOTCH1 (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase

    Plasmid

    #188391

    Purpose

    Reporter for BCAM expression

    Depositor

    Markus Frank

    Article

    Sasamoto et al Cell Rep. 2022 Aug 9;40(6):111166. doi:

    Insert

    BCAM reporter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGP-CMV-jGCaMP7s

    Plasmid

    #104463

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7s
    

    Use

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV-CAG-jGCaMP8s-WPRE

    Plasmid

    #179256

    Purpose

    AAV-mediated expression of GCaMP8s under the CAG promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMXs-Hhex

    Plasmid

    #131600

    Purpose

    expression of mouse Hhex in mammalian cells

    Depositor

    Filipe Pereira, Kateri Moore

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Hhex (Hhex Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR223_BTK_WT

    Plasmid

    #82197

    Purpose

    Gateway Donor vector containing BTK , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    BTK (BTK Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLJM1-EGFP-GRAM-H

    Plasmid

    #211703

    Purpose

    Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (pLJM1-EGFP-GRAM1b G187L)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGL2-Skp2 promoter-luciferase

    Plasmid

    #81119

    Purpose

    Reporter activity of SKP2 gene promoter

    Depositor

    Sung Hee Baek

    Article

    Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

    Insert

    SKP2 (Skp2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    contains the region from 1_kb upstream of transcr…

    Promoter

    SKP2

    Available since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

33. pHAGE-FLAG-APEX2-MAP1LC3B

    Plasmid

    #175753

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a(+) NRP1 b1 (273-427)

    Plasmid

    #162734

    Purpose

    Bacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

    Depositor

    Brett Collins

    Article

    Daly et al Science. 2020 Oct 20. pii: science.abd30

    Insert

    Human NRP1 b1 domain residues 273-427
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimised

    Promoter

    Available since

    April 27, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. α-actinin-4-M522-sstFRET(Venus/Cerulean) in pUNO1

    Plasmid

    #190799

    Purpose

    Imaging of force-sensitive α-actinin-4 in live cells

    Depositor

    Vivian Tang

    Article

    Morris et al J Cell Biol. 2022 May 2;221(5). pii: 213

    Insert

    α-actinin-4-Venus-Cerulean-522 (ACTN4 Human, Synthetic)
    

    Use

    Tags

    cerulean and venus

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1-HTLV

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GFP-hCCDC11

    Plasmid

    #122871

    Purpose

    Labels centriolar satellites in mammalian cells

    Depositor

    Ken-Ichi Takemaru, FENG-QIAN Li

    Insert

    human CCDC11 (CFAP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJ204-loxP-100RPU.2-ETN-BGHpA-cHS4-100RPU.2-ETN-lox2272

    Plasmid

    #154834

    Purpose

    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of etanercept

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Inserts

    ETN
    ETN
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    100RPU.2

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GFP-CA-mDia1

    Plasmid

    #45583

    Depositor

    Klaus Hahn, Ronen Zaidel-Bar

    Article

    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

    Insert

    Constitutively active mDia1 (CA-mDia1) (Diaph1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Insert mDia1 contains mutations V161D and N165D i…

    Promoter

    CMV

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

39. psiCHECK2-miR-106b-5p

    Plasmid

    #135713

    Purpose

    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-106-5p)

    Depositor

    Florian Karreth

    Insert

    miR-106b-5p reverse complement
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GFP-RalGDS

    Plasmid

    #118315

    Purpose

    Expression of human RalGDS

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Insert

    RalGDS (RALGDS Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    fragment of human RalGDS 203aa

    Promoter

    CMV

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pHR_SFFv_Her2-BFP_RHL027

    Plasmid

    #164823

    Purpose

    Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    Extracellular and transmembrane region of ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. psiCHECK2-miR-144-3p

    Plasmid

    #135717

    Purpose

    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-144-3p)

    Depositor

    Florian Karreth

    Insert

    miR-144-3p reverse complement
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAGC-Lb12-P1Bb

    Plasmid

    #231387

    Purpose

    For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

    Depositor

    Qi-Jun Chen

    Article

    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

    Insert

    U6-29
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGL3-mBcl11b-MAR4

    Plasmid

    #178188

    Purpose

    Lucifease reporter driven by MAR4 regulatory sequences of mouse Bcl11b

    Depositor

    Katsuhiko Ono

    Article

    Nomura et al Cell Rep. 2018 Mar 20;22(12):3142-3151.

    Insert

    Bcl11b MAR4
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 promoter

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. pGL3-Ub(L40)

    Plasmid

    #52892

    Purpose

    Aedes aegypti ubiquitin promoter driving firefly luciferase

    Depositor

    Zach Adelman

    Article

    Anderson et al Insect Mol Biol. 2010 Aug;19(4):441-9. d

    Insert

    ubiquitin promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Ae aegypti ubiquitin

    Available since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pDONR221-SFXN1

    Plasmid

    #132311

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SFXN1 (SFXN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. pBad-CA

    Plasmid

    #105665

    Purpose

    expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA 3.2/V5-DEST CDC42EP1 S192A

    Plasmid

    #69820

    Purpose

    Expresses CDC42EP1-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    CDC42 effector protein (CDC42EP1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S192A

    Promoter

    CMV

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. psiCHECK2-miR-19-3p

    Plasmid

    #135710

    Purpose

    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-19-3p)

    Depositor

    Florian Karreth

    Insert

    miR-19-3p reverse complement
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLenti Lifeact-iRFP670 BlastR

    Plasmid

    #84385

    Purpose

    Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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