We narrowed to 16,885 results for: Por
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Plasmid#216373PurposexhNup214-Nb1t (+2Cys) Nanobody productionDepositorInsertxhNup214-Nb1t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-scSUMOExpressionBacterialMutationPromoterAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-PtH2B
Plasmid#133390PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterNo promoterAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-MTS-EGFP
Plasmid#190270PurposeLentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cellsDepositorInsertMTS-EGFP
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterTight TRE promoterAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ3H PTP-1B
Plasmid#8601DepositorAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.21_PB-UGCG-PGK-Blast
Plasmid#205566PurposeExpress human UGCGDepositorAvailable SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-BFP
Plasmid#138272PurposeExpression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editorDepositorInsertBFP H66 PAM
UseCRISPRTagsExpressionMammalianMutationH66, PAM motifPromoterEf1aAvailable SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-CPspaH2B
Plasmid#133385PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HaloTag-CNA35
Plasmid#215457PurposeEncodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.DepositorInsertCNA35
UseTagsHaloTagExpressionBacterialMutationIncludes stop codonPromoterAvailable SinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFSKm4-ATF1
Plasmid#221133PurposeFormaldehyde sensor based on the frmR repressor, which expresses yeast alcohol acetyl transferase (ATF1) upon detectionDepositorAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sender_plasmid2
Plasmid#140693PurposeEncodes inducible expression of RhlI (C4-HSL quorum sensing synthase)DepositorInsertRhlI (rhlI )
UseTagsssrA degradation tag (ASV)ExpressionBacterialMutationPromoterengineered lac promoter (IPTG inducible)Available SinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
UseTagsExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAL101
Plasmid#22474PurposeExpresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to PhyDepositorInsertPIF6APB
UseSynthetic BiologyTagsmYFPExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015
Plasmid#202025PurposeExpresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coliDepositorInsertBacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
UseTagsExpressionBacterialMutationGvpB S9G-R31L-R85LPromoterAvailable SinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP728
Plasmid#216374PurposexNup358-Nb1t (+3Cys) Nanobody productionDepositorInsertxNup358-Nb1t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Slow-FT
Plasmid#31915DepositorInsertSlow-FT
UseTagsExpressionMammalianMutationM18V/E30V/K69R/L84W/A179V compared to mCherry (bu…PromoterPTight Tet-responsive PminCMVdAvailable SinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_BirA
Plasmid#136537PurposeLentiviral expression vector for an inducible BirADepositorInsertBirA
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationPromoterAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
P2A-NLS-tdtomato-EPG backbone
Plasmid#215548PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
P2A-mCherry-EhT backbone
Plasmid#215549PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomatoDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai
Plasmid#202569PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Arginine 261 to AlaninePromoterAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3-Neo-EF1a-GFP
Plasmid#69134PurposeSleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promotersDepositorTypeEmpty backboneUseSleeping beauty transposonTagsExpressionMammalianMutationPromoterU3MSCVAvailable SinceJan. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRXG
Plasmid#113643PurposeThis construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.DepositorInsertmRFP1-linker-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR
Plasmid#192636PurposeExpresses ERT2-CreN59-nMag-NLS with mCherry marker.DepositorInsertERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mut1-RS
Plasmid#182881PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertMut1-RS
UseTagsExpressionBacterialMutationPromoterAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-ASAP1
Plasmid#52519PurposeFor mammalian expression of the fluorescent voltage sensor ASAP1DepositorInsertAccelerated Sensor of Action Potentials 1
UseTagsExpressionMammalianMutationPromoterCAGAvailable SinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCSC-NLS-EGFP
Plasmid#221811PurposeLentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)DepositorInsertNLS-EGFP
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-epNTR
Plasmid#62213PurposeepNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2DepositorInsertepNTR
UseMrna synthesisTagsExpressionMutationPromoterAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFast-FT-N1
Plasmid#31910DepositorInsertFast-FT
UseTagsExpressionMammalianMutationM18V/E34K/K69R/L84W/S149T/A224S compared to mCher…PromoterCMVAvailable SinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
T2A 3xNLS GFP
Plasmid#227721PurposePlasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertT2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
UseCloning vectorTagsExpressionMutationPromoterAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
P336-FBP_11
Plasmid#139702PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing LuzDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_g5-HT3.0
Plasmid#208727PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytesDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTK-RetE1-Citrine
Plasmid#127779PurposeEnhancer reporter construct containing the chicken RetE1 enhancer driving Citrine activityDepositorInsertRetE1
UseEnhancer reporter constructTagsExpressionMutationPromoterAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG-NSarQ27G-VMAn-FKBP
Plasmid#70229PurposeExpresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicingDepositorInsertalpha Sarcin (N-terminal portion)
UseTags3XFLAG, FK506- and rapamycin-binding protein (FKB…ExpressionMammalianMutationChanged Gln 27 to GlyPromoterCMVAvailable SinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTRrsGreenF-E
Plasmid#205764PurposeExpression of JNK KTR rsGreenF-E under PKG promoter (With Puromycin Resistance)DepositorInsertJNK KTR rsGreenF-E
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV Puro DEST ERKKTRrsFastLime
Plasmid#205765PurposeExpression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)DepositorInsertERK KTR rsFastLime
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
AzoRS-4
Plasmid#182884PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertAzoRS-4
UseTagsExpressionBacterialMutationPromoterAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)::NL
Plasmid#141289PurposeNLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC
UseLuciferaseTagsExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH2.155_LV-Puro-T2A-mTagBFP2
Plasmid#205553PurposeExpress puromycin resistance marker and mTagBFP2DepositorInsertPuro-T2A-mTagBFP2
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-QFGal4-SV40pA
Plasmid#155118PurposeMiddle entry vector containing QFGal4DepositorInsertQFGal4
UseGateway middle entry vectorTagsExpressionMutationPromoterAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Medium-FT
Plasmid#31914DepositorInsertMedium-FT
UseTagsExpressionMammalianMutationM18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…PromoterPTight Tet-responsive PminCMVdAvailable SinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-GRAB_g5-HT3.0
Plasmid#208726PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVTagsExpressionMutationPromoterEF1aAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB c Rosella I
Plasmid#71245Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.DepositorInsertRosella
UseTagsExpressionYeastMutationPromoterPGK1Available SinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-NCreERT2
Plasmid#106370PurposeN-terminal part of the split-CreERT2 SystemDepositorInsertNCreERT2
UseCre/LoxTagsflagExpressionMammalianMutationPromoterCMVAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag3B-ERT2CCre
Plasmid#106371PurposeC-terminal part of the split-CreERT2 SystemDepositorInsertERT2CCre
UseCre/LoxTagsc-mycExpressionMammalianMutationPromoterCMVAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-GFP1-9 T2A mCherry
Plasmid#124430PurposeExpresses GFP1-9 and T2A mCherry in mammalian cellsDepositorInsertGFP1-9 T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKV016
Plasmid#64767PurposeRecombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1
Plasmid#214995PurposeExpress the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.DepositorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMedium-FT-N1
Plasmid#31911DepositorInsertMedium-FT
UseTagsExpressionMammalianMutationM18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…PromoterCMVAvailable SinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
Plasmid#176311PurposeExpresses NLS-pMag-CreC60 with tagBFP markerDepositorInserttagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
UseLentiviral and Synthetic BiologyTagstagBFPExpressionMammalianMutationPromoterhuman EF1alphaAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG385 bJun-NFAST-NLS-DEVDG-mCherry-NES
Plasmid#130816PurposeExpresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cellsDepositorInsertbJun-NFAST-NLS-DEVDG-mCherry-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUBC_hsPUM1-mCherry
Plasmid#107607PurposeTransient expression of Pumilio1, fused to mCherry in mammalian cells, under a hUBC promoterDepositorInserthsPUM1-mCherry
UseSynthetic BiologyTagsmCherryExpressionMammalianMutationPromoterhuman UBCAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only