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We narrowed to 24,628 results for: Nes

Showing: 4121 - 4160 of 24628 results
  1. pKL2299

    Plasmid
    #186332
    Purpose
    This plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.
    Depositor
    Insert
    virulence genes from Agrobacterium tumefaciens pTiBo542
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3

    Plasmid
    #111924
    Purpose
    Orange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μ
    Depositor
    Insert
    OeNL(Ca2+)_18μ
    Use
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, E104D, D133E at CaM
    Promoter
    CMV
    Available Since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

    Plasmid
    #111925
    Purpose
    Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ
    Depositor
    Insert
    CeNL(Ca2+)_110μ
    Use
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    E31D, F92W, E104D. D133E at CaM
    Promoter
    CMV
    Available Since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

    Plasmid
    #111923
    Purpose
    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ
    Depositor
    Insert
    CeNL(Ca2+)_19μ
    Use
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, F92W, E104D, D133E at CaM
    Promoter
    CMV
    Available Since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pDule-Acd82

    Plasmid
    #174099
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Promoter
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pAAV-hSyn-sbGLuc-B7-EYFP

    Plasmid
    #114110
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics
    Inserts
    Gaussia luciferase, slow burn
    B7 hinge region
    Enhanced Yellow Fluorescent Protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pV1524

    Plasmid
    #111431
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
    Depositor
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pDule2-Acd82

    Plasmid
    #174100
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Promoter
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pV1393

    Plasmid
    #111430
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pCRISPathBrick

    Plasmid
    #65006
    Purpose
    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive native promoters
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. AAEL010097-Cas9

    Plasmid
    #100707
    Purpose
    The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Inserts
    AAEL010097-hspcas9-GFP
    Opie2-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Mutation
    Promoter
    AAEL010097 and Opie2
    Available Since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  12. pEasyG2_mic

    Plasmid
    #184916
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.
    Depositor
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pC1-SypHer3s-IMS

    Plasmid
    #108120
    Purpose
    Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.
    Depositor
    Insert
    SypHer3s-IMS
    Use
    Tags
    ratiometricYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 4121 - 4160 of 24628 results