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61,345 results

Showing: 5251 - 5300 of 61345 results

  2. pD649-HAsp-SEMA7A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156922
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SEMA7A (SEMA7A Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156922-plasmid-map-sequence-id-502718

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  5. pRRlsinPGK_H2BGFP_WPRE

    Plasmid
    #91788
    Purpose
    Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.
    Depositor
    Beverly Torok-Storb
    Insert
    Human histone H2B (H2BC11 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91788-plasmid-map-sequence-id-180614

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  7. SPLICSFAST-NU-MT-P2A

    Plasmid
    #214898
    Purpose
    Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-NU-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214898-plasmid-map-sequence-id-423484

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  11. pAAV-hSyn-hChR2(H134R)-EYFP

    Plasmid
    #26973
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available Since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26973-plasmid-map-sequence-id-199313

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  13. TFORF3439

    Plasmid
    #144915
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXI1 (FOXI1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144915-plasmid-map-sequence-id-404496

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  14. TFORF1207

    Plasmid
    #144089
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CASZ1 (CASZ1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144089-plasmid-map-sequence-id-396173

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  19. pCMV-dCas13-NES-eNAT10

    Plasmid
    #238299
    Purpose
    Expresses cytoplasmic version of the programmable RNA acetylation system.
    Depositor
    Won Do Heo
    Article
    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/
    Insert
    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    Use
    CRISPR
    Tags
    HIV NES (C terminal of dPspCas13b)
    Expression
    Mammalian
    Mutation
    H133A for catalytically inactive mutant, deletion…
    Available Since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    238299-plasmid-map-sequence-id-472414

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  22. LYSO-LRRK2

    Plasmid
    #193582
    Purpose
    Expresses human full-length LRRK2 with a lysosomal targeting sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminus
    Depositor
    Mark Cookson
    Article
    Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    Leucine-rich repeat kinase 2 (LRRK2 Human)
    Tags
    LAMTOR1(1-39aa) and 3xFLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    193582-plasmid-map-sequence-id-382173

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  23. pBabepuro-IL11

    Plasmid
    #189609
    Purpose
    Retroviral expression vector for IL11
    Depositor
    Thierry Tchenio
    Insert
    IL11 (IL11 Human)
    Use
    Retroviral
    Promoter
    SV40 early promoter
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189609-plasmid-map-sequence-id-381093

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  28. TFORF2543

    Plasmid
    #142957
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX5 (SOX5 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142957-plasmid-map-sequence-id-397287

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  32. mEos3.2-TOMM20-N-10

    Plasmid
    #57483
    Purpose
    Localization: Mitochondrial Membrane, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    TOMM20 (TOMM20 Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50454
    Purpose
    Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV8
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50454-plasmid-map-sequence-id-214300

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  35. mTagRFP-T2-PCNA-19

    Plasmid
    #58043
    Purpose
    Localization: Nucleus, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Insert
    PCNA (PCNA Human)
    Tags
    mTagRFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    58043-plasmid-map-sequence-id-175700

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  36. V2-STING

    Plasmid
    #124265
    Purpose
    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus2
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124265-plasmid-map-sequence-id-241431

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  47. huntingtin_FL_NbioCflag

    Plasmid
    #194767
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    Q23 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    July 27, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194767-plasmid-map-sequence-id-402615

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  48. TFORF1219

    Plasmid
    #143939
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PRRX2 (PRRX2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143939-plasmid-map-sequence-id-402645

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Showing: 5251 - 5300 of 61345 results