Purpose
              
              Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.
             
          
          
          
            
          
        
          
  
  
    
      
        Inserts
      
      
        
          Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
        
          Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
        
          deGFP
        
      
    
   
  
  
    
  
  
    
  
  
    
  
  
    
      
        Mutation
      
      Changed tyrosine 35 to TAG stop-codon (Site numbe…
     
  
  
    
      
        Promoter
      
      P70b promoter and T500 terminator, Vibrio natrieg…
     
  
         
          
            
              
                Available Since
              
              
                May 11, 2021
              
             
          
          
            
              Availability
            
            
              
                Academic Institutions and Nonprofits only