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We narrowed to 591 results for: beta-actin

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Showing: 501 - 550 of 591 results

  12. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid
    #182680
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182680-plasmid-map-sequence-id-363164

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  13. pHLmMBP-8

    Plasmid
    #72347
    Purpose
    Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72347-plasmid-map-sequence-id-133755

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  15. pHLmMBP-11

    Plasmid
    #72349
    Purpose
    Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72349-plasmid-map-sequence-id-133760

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  17. pHLmMBP-1

    Plasmid
    #72343
    Purpose
    Mammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72343-plasmid-map-sequence-id-132066

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  18. pHLmMBP-3

    Plasmid
    #72345
    Purpose
    Mammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72345-plasmid-map-sequence-id-133752

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  21. pBa-tdTomato-flag-BicD2 594-FKBP

    Plasmid
    #64205
    Purpose
    Bicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBP
    Depositor
    Gary Banker, Marvin Bentley
    Article
    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.
    Insert
    Bicaudal D protein (Bicd2 Mouse)
    Tags
    FKBP, flag, and tdTomato
    Expression
    Mammalian
    Mutation
    no start methionine, aa1-594
    Promoter
    chicken Beta Actin
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    64205-plasmid-map-sequence-id-107603

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  22. pHLmMBP-6

    Plasmid
    #72346
    Purpose
    Mammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72346-plasmid-map-sequence-id-133754

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  23. pBa-eGFP-flag-BicD2 594-FKBP

    Plasmid
    #63569
    Purpose
    Bicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBP
    Depositor
    Gary Banker, Marvin Bentley
    Article
    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.
    Insert
    Bicaudal D protein (Bicd2 Mouse)
    Tags
    FKBP, eGFP, and flag
    Expression
    Mammalian
    Mutation
    no start methionine, aa1-594
    Promoter
    chicken Beta Actin
    Available Since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    63569-plasmid-map-sequence-id-105705

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  25. pHLmMBP-2

    Plasmid
    #72344
    Purpose
    Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72344-plasmid-map-sequence-id-132067

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  35. pCAG CAPN7 V18D,F98D-OSF

    Plasmid
    #193045
    Purpose
    Mammalian expression plasmid encoding full-length CAPN7 with V18D and F98D mutations that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    CAPN7 (CAPN7 Human)
    Tags
    OSF (One-Strep-FLAG)
    Expression
    Mammalian
    Mutation
    V18D, F98D
    Promoter
    Chicken Beta-actin
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193045-plasmid-map-sequence-id-378994

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  38. TKIT NFL gRNA1 gRNA2

    Plasmid
    #182681
    Purpose
    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182681-plasmid-map-sequence-id-363020

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  39. pBa-LSS-GFP-LDLR wt

    Plasmid
    #98184
    Purpose
    Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.
    Depositor
    Gary Banker, Marvin Bentley
    Article
    Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.
    Insert
    LDLR (LDLR Human)
    Tags
    GFP and LDLR signal sequence, N-term of GFP so GF…
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Chicken Beta Actin
    Available Since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98184-plasmid-map-sequence-id-490370

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  40. pHLmMBP-10

    Plasmid
    #72348
    Purpose
    Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72348-plasmid-map-sequence-id-133757

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Showing: 501 - 550 of 591 results