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We narrowed to 6,502 results for: poly

Showing: 5501 - 5550 of 6502 results

  1. pDB007ns2a

    Plasmid
    #99214
    Purpose
    Accessory plasmid for PACE of PylRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and Ptet
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99214-plasmid-map-sequence-id-192369

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  3. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Mutation
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47912-plasmid-map-sequence-id-68534

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  4. ERAP1

    Plasmid
    #39174
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ERAP1 (ERAP1 Human)
    Use
    Baculovirus expression
    Tags
    TEV-His10-Flag
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pDB023f1

    Plasmid
    #99218
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99218-plasmid-map-sequence-id-192402

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  13. pET15bLCFPT7

    Plasmid
    #53545
    Purpose
    Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant
    Depositor
    Lingchong You
    Article
    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10
    Insert
    T7 RNAP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    53545-plasmid-map-sequence-id-83442

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  14. pGBW-m4092709

    Plasmid
    #153859
    Purpose
    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Nearly native encoding;handful SNPs as watermarks…
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153859-plasmid-map-sequence-id-301594

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  15. DPYSL4 (DPYSL4A-c003)

    Plasmid
    #110263
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DPYSL4 (DPYSL4 Human)
    Use
    Tags
    His6-ZBasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110263-plasmid-map-sequence-id-212341

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  18. pDB023k

    Plasmid
    #99219
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] T7RNAP(S12*,S203*,S527*)
    Use
    Tags
    Expression
    Mutation
    amber codons at positions 12, 203, and 527 of T7 …
    Promoter
    Ppsp
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99219-plasmid-map-sequence-id-192936

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  19. ABCB8 (ABCB8A-c016)

    Plasmid
    #110266
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ABCB8 (ABCB8 Human)
    Use
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110266-plasmid-map-sequence-id-212276

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  21. pCRUSH

    Plasmid
    #54480
    Purpose
    Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox conditional U6 promoter pgk-puro
    Depositor
    Laurie Jackson-Grusby
    Article
    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and RNAi
    Tags
    SA-EGFP-polyA, U6 promoter, loxP, and pgk-puro
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  22. pCoofy41

    Plasmid
    #55184
    Purpose
    Insect expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…
    Expression
    Bacterial
    Mutation
    Promoter
    pPH (Polyhedrin promoter)
    Available since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    55184-plasmid-map-sequence-id-464534

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  23. 5WCJ (METTL13)

    Plasmid
    #101270
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    METTL13 (METTL13 Human)
    Use
    Baculovirus expression
    Tags
    6xHis-TEV
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    Oct. 30, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101270-plasmid-map-sequence-id-197531

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  24. ataxin3:1-431_Q79_BV

    Plasmid
    #209733
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    6xHis-SBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Polyhedrin
    Available since
    Nov. 13, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    209733-plasmid-map-sequence-id-414202

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  25. RBBP4

    Plasmid
    #25350
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RBBP4-13 (RBBP4 Human)
    Use
    Baculovirus expression
    Tags
    His
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. 5THA (Gemin5)

    Plasmid
    #101890
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Gemin5 (GEMIN5 Human)
    Use
    Baculovirus expression
    Tags
    6xHis, Thrombin cleavage
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101890-plasmid-map-sequence-id-194723

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  29. CYP7A1 (3SN5)

    Plasmid
    #32874
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CYP7A1 (CYP7A1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amino acids 25-503 only
    Promoter
    Available since
    Jan. 13, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. pMT440

    Plasmid
    #8609
    Depositor
    Robert Hartley
    Article
    Yazynin et al Gene 1996 Feb 22;169(1):131-2.
    Insert
    barnase, barstar
    Use
    Tags
    as pMT416
    Expression
    Bacterial
    Mutation
    V36 codon of barnase of pMT416 replaced by polyli…
    Promoter
    Available since
    Jan. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  36. AASS (AASSA-c013)

    Plasmid
    #98227
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AASSA (AASS Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Expression
    Mutation
    pfam03435:Saccharop_dh 483-916
    Promoter
    polyhedrin
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98227-plasmid-map-sequence-id-193306

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  37. ACVR1 (ACVR1Z-c041)

    Plasmid
    #110269
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACVR1 (ACVR1 Human)
    Use
    Tags
    His-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110269-plasmid-map-sequence-id-212278

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  46. DENND1A (DENND1AA-c032)

    Plasmid
    #110270
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DENND1A (DENND1A Human)
    Use
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110270-plasmid-map-sequence-id-212279

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  47. pTE5425

    Plasmid
    #229032
    Purpose
    Expression vector for 10xHis-phi29 DNA polymerase (catalytically inactive mutant D249E): PT7-lacO-g10leader-p2 dead-T7 terminator, CamR
    Depositor
    Tobias Erb
    Article
    Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/
    Insert
    PT7-lacO-g10lead-10xHisTag-phi29 DNAP (D249E, catalytically inactive)
    Use
    Tags
    10x His-tag + Factor Xa recognition site
    Expression
    Bacterial
    Mutation
    Promoter
    PT7-lacO and T7 promoter
    Available since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    229032-plasmid-map-sequence-id-466060

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Showing: 5501 - 5550 of 6502 results