19,190 results

1. pCDH-EF1a-eFFly-eGFP

   Plasmid

   #104834

   Purpose

   eFFly luciferase for invivo Imaging, eGFP for flourescent detection

   Depositor

   Irmela Jeremias

   Article

   Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

   Inserts

   eFFly
   eGFP
   

   Use

   Lentiviral and Luciferase; Egfp

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pveg

   Plasmid

   #55173

   Purpose

   Very strong constitutive promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   veg promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   veg

   Available since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNPK-NLS-Vika

   Plasmid

   #79969

   Purpose

   expresses human codon-optimized Vika-recombinase in mammalian cells

   Depositor

   Frank Buchholz

   Article

   Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

   Insert

   human codon-optimized Vika sequence, NLS tagged
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS_cAMPFIRE-H

   Plasmid

   #182285

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-H (cAMP sensor)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MaLionG

   Plasmid

   #113906

   Purpose

   Green ATP sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

   Insert

   MaLionG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-SEP-GluA1 TKIT

   Plasmid

   #169441

   Purpose

   Expression of 2 guides + donor DNA

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9 TadCBEd

   Plasmid

   #193835

   Purpose

   Express TadCBEd (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDd-SpCas9 D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSP64T (DM#111)

   Plasmid

   #15030

   Depositor

   Douglas Melton

   Article

   Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

   Insert

   beta-globin 5' and 3' UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. Venus-pBAD

   Plasmid

   #54859

   Purpose

   Localization: Protein Expression Vector , Excitation: 515, Emission: 528

   Depositor

   Atsushi Miyawaki, Michael Davidson

   Article

   Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. HA GSK3 beta S9A pcDNA3

    Plasmid

    #14754

    Purpose

    Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

    Depositor

    Jim Woodgett

    Article

    Stambolic et al Biochem J. 1994 Nov 1. 303 ( Pt 3)():701

    Insert

    GSK3 beta S9A (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S9A. Constitutively active mutant.

    Promoter

    Available since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3.1 mycBioID

    Plasmid

    #35700

    Purpose

    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    cmv

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLT3-MSCV-IRES-GFP

    Plasmid

    #184778

    Purpose

    Expresses wildtype FLT3 in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE60-EFG-His

    Plasmid

    #124126

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Elongation factor G (fusA Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ApoE4

    Plasmid

    #197148

    Purpose

    Expresses ApoE4 in Mammalian cell

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE4 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-sgRNA hygro

    Plasmid

    #104991

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-PS-Grb2 (XSB363)

    Plasmid

    #78505

    Purpose

    Construct for expressing Grb2 in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Grb2 (GRB2 Human)
    

    Use

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiV2-dCas9-VP64

    Plasmid

    #141104

    Purpose

    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

    Depositor

    Igor Ulitsky

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR4-FLAG (w210-2)

    Plasmid

    #17423

    Purpose

    Gateway entry vector for N-term FLAG tag

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29b-IPP1-His

    Plasmid

    #124137

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Inorganic pyrophosphatase (IPP1 Budding Yeast)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL410_Ins2500

    Plasmid

    #49058

    Purpose

    Expresses luciferase under control of mouse insulin 2 gene promoter

    Depositor

    Kevin Ferreri

    Article

    Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

    Insert

    mouse Ins2 promoter (Ins2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ins2 promoter

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMD.RVG.CVS24-B2c

    Plasmid

    #19713

    Depositor

    Manfred Schubert

    Article

    Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

    Insert

    Rabies virus CVS24-B2c glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FRE5Cf

    Plasmid

    #62378

    Purpose

    Derived from FRE5. A two nucleotide insertion to shift the reading frame to dsRED.

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    dsRed and GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCR4-24XMS2SL-stable

    Plasmid

    #31865

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    24xMS2 Stem Loops - Stable
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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24. dCas13d-dsRBD

    Plasmid

    #154938

    Purpose

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCL-CTIG

    Plasmid

    #14901

    Purpose

    3rd generation lentiviral vector expressing tTA-VP16 and EGFP.

    Depositor

    Inder Verma

    Article

    Kafri et al Mol Ther. 2000 Jun . 1(6):516-21.

    Insert

    CMV-tTA-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLL3.7 EGFPC2 TAZ

    Plasmid

    #66850

    Purpose

    Express GFP-fused TAZ by lentivirus

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    WWTR1/TAZ (WWTR1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-C1-NuMA

    Plasmid

    #81029

    Purpose

    Expresses human NuMA with N-terminal EGFP tag in mammalian cells

    Depositor

    Michael Mancini

    Insert

    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-N1-IL6

    Plasmid

    #111933

    Purpose

    Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Interleukin 6 (IL6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    CMV

    Available since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209722

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCIP-AMPKa1_KD

    Plasmid

    #79011

    Purpose

    For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47R

    Promoter

    pCAGGs

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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