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61,355 results

Showing: 6001 - 6050 of 61355 results

  4. pLKO.1 puro-humanU6-shRNA FASN

    Plasmid
    #82327
    Purpose
    used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)
    Depositor
    Elizabeth Stoll
    Insert
    shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82327-plasmid-map-sequence-id-155713

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  5. LEGO-AP1x6-GM55

    Plasmid
    #217415
    Purpose
    Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x6
    Depositor
    Peter Cockerill
    Insert
    Luciferase, GFP
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    217415-plasmid-map-sequence-id-441062

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  8. dCas9-dMSK1

    Plasmid
    #165602
    Purpose
    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)
    Depositor
    Isaac Hilton
    Article
    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1
    Insert
    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Synthetic, Human, S. Pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    165602-plasmid-map-sequence-id-327024

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  13. TFORF2079

    Plasmid
    #141911
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FLI1 (FLI1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141911-plasmid-map-sequence-id-397213

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  17. HA-Rab7A

    Plasmid
    #131417
    Purpose
    Expresses HA-tagged human Rab7A in mammalian cells
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Insert
    human Rab7A
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131417-plasmid-map-sequence-id-258194

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  18. CCR8-DuET

    Plasmid
    #213204
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR8 (CCR8 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213204-plasmid-map-sequence-id-430767

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  19. pD649-HAsp-CD19-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156714
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CD19 (CD19 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156714-plasmid-map-sequence-id-407030

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  20. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid
    #229680
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    SNAP, V5
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229680-plasmid-map-sequence-id-457075

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  22. hNTo2-qgRNA-pYJA5

    Plasmid
    #217782
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217782-plasmid-map-sequence-id-466020

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  27. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid
    #221002
    Purpose
    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    dCasRx-RBM25-IRES-BleoR
    Use
    CRISPR
    Tags
    NLS and NLS-HA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    221002-plasmid-map-sequence-id-436162

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  33. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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  34. HaloTag-STAG1 Vector

    Plasmid
    #238689
    Purpose
    Express HaloTag-STAG1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    STAG1 (STAG1 Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238689-plasmid-map-sequence-id-479895

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  35. pCB98

    Plasmid
    #69744
    Purpose
    Retroviral expression of Centrin 1
    Depositor
    Iain Cheeseman
    Insert
    Centrin 1 (CETN1 Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Available Since
    Nov. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  42. pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald

    Plasmid
    #225949
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    C100A
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225949-plasmid-map-sequence-id-446851

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  43. pLV-SFFV-CKAP4 CC-WPRE-UbC-Emerald

    Plasmid
    #225950
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation double cysteine (CC) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Additional cysteine inserted after Cys-100
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225950-plasmid-map-sequence-id-446852

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  44. PTGDR-DuET

    Plasmid
    #213369
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    PTGDR (PTGDR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213369-plasmid-map-sequence-id-430728

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  45. pmCer-RecQL4

    Plasmid
    #211400
    Purpose
    mammalian expression of human RecQL4 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    RecQL4 (RECQL4 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211400-plasmid-map-sequence-id-420508

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Showing: 6001 - 6050 of 61355 results