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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 6051 - 6100 of 12598 results

  1. pKM197

    Plasmid
    #132665
    Purpose
    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
    Depositor
    Joseph Sorg
    Article
    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:
    Insert
    Upstream & Downstream pyrE deletion region
    Use
    E. coli - c. difficile shuttle vector
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132665-plasmid-map-sequence-id-259679

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  25. pGP-Tn7-Pcym

    Plasmid
    #225280
    Purpose
    Mini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.
    Depositor
    Mark Schembri
    Article
    Chang et al Appl Environ Microbiol. 2024 Oct 23;90(1
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    Pcym cumate (p-isopropylbenzoate)-inducible promo…
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    225280-plasmid-map-sequence-id-457260

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  31. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available Since
    Sept. 14, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68710-plasmid-map-sequence-id-123992

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  38. pNR64

    Plasmid
    #79540
    Purpose
    Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.
    Depositor
    Timothy Lu
    Article
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    TP901
    BxbI
    Tags
    His tag and LAA degradation tag
    Expression
    Bacterial
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79540-plasmid-map-sequence-id-148741

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Showing: 6051 - 6100 of 12598 results