165,947 results

1. IF-GFP-ATRX

   Plasmid

   #45444

   Purpose

   ATRX expression vector

   Depositor

   Michael Dyer

   Article

   Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

   Insert

   ATRX (ATRX Human, Mouse)
   

   Tags

   GFP and HA

   Expression

   Bacterial and Mammalian

   Mutation

   isoform 2, missing codon E124

   Promoter

   PCMV

   Available Since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn1-FLEX-CybGam-WPRE

   Plasmid

   #207662

   Purpose

   Control for CybSEP2

   Depositor

   Sung Han

   Article

   Kim et al bioRxiv 2023.01.19.524797

   Insert

   Gamillus
   

   Use

   AAV

   Mutation

   Histidine 86 and 159 to alanines, inserted gamill…

   Promoter

   hSynapsin

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PH-Akt-GFP

   Plasmid

   #51465

   Purpose

   Is a biosensor for PIP3/PI(3,4)P2

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   AKT (aa 1–164)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. CL20_mEGFP_TMPRSS2_ERG

   Plasmid

   #205943

   Purpose

   Express mEGFP-tagged fusion protein, TMPRSS2_ERG from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   TMPRSS2_ERG (TMPRSS2, ERG Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. MIGR1

   Plasmid

   #27490

   Depositor

   Warren Pear

   Article

   Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. R57C10-Flp2

   Plasmid

   #63172

   Purpose

   R57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusion

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   yeast wild type containing a Glycine at amino acid 5 of the protein sequence
   

   Expression

   Insect

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pRSET Nucleolin

   Plasmid

   #13037

   Depositor

   Peter Newburger

   Article

   Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

   Insert

   Nucleolin (NCL Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV GFP DEST (736-1)

   Plasmid

   #19732

   Purpose

   3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi; Gateway destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213027

   Purpose

   Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
   

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   D702Y (RT- reverse transcriptase catalytic dead)

   Promoter

   CMV

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-PGK-Venus-Akaluc (neo)

    Plasmid

    #124701

    Purpose

    Aka-Luciferase reporter

    Depositor

    Roland Friedel

    Article

    Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

    Insert

    Venus-Akaluciferase
    

    Use

    Lentiviral

    Promoter

    hPGK

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPRDEnv

    Plasmid

    #1732

    Purpose

    This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab

    Depositor

    Garry Nolan

    Insert

    Gag-Pol and Rev
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMIIS793

    Plasmid

    #238206

    Purpose

    Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    TaTasR
    

    Tags

    TwinStrepII-bdSUMO

    Expression

    Bacterial

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. JD633

    Plasmid

    #160393

    Purpose

    CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Inserts

    ZmUbi::wheat GRF4-GIF1 chimera
    ZmUbi:TaCas9 + TaU6:gRNA
    

    Use

    Binary vector

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-GAVPO

    Plasmid

    #159923

    Purpose

    GAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    GAVPO
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-opto-hCaspase-1

    Plasmid

    #208790

    Purpose

    Inducibly expresses human Caspase1, fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Caspase1 (CASP1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158652

    Purpose

    making transgenics expressing a UAS driven eGFP and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Mutation

    F70A/F108Y

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-N-FLAG-HA-TBK1

    Plasmid

    #131791

    Purpose

    Lentiviral vector expressing WT TBK1

    Depositor

    Alicia Pickrell

    Article

    Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

    Insert

    TBK1 WT (TBK1 Human)
    

    Use

    Lentiviral

    Tags

    N-FLAG-HA

    Expression

    Mammalian

    Mutation

    WT full length

    Promoter

    CMV

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-myc-GLUT4-mCherry

    Plasmid

    #64049

    Purpose

    3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    GLUT4 (Slc2a4 Mouse)
    

    Use

    Lentiviral

    Tags

    c-Myc and mCherry

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ144

    Plasmid

    #69296

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCWU6-xylR-E-luciferase

    Plasmid

    #190721

    Purpose

    fine tune expression of luciferase under the control of xylose- and theophylline- inducible system

    Depositor

    Chenggang Wu

    Article

    Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

    Insert

    xylR-E-luciferase
    

    Use

    Luciferase

    Expression

    Bacterial

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDONR223_TRAP1_WT_V5

    Plasmid

    #82994

    Purpose

    Gateway Donor vector containing TRAP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    TRAP1 (TRAP1 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCRISPaint-TagBFP-PuroR

    Plasmid

    #80969

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    TagBFP
    

    Use

    Gene tagging

    Promoter

    none

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-CAG-mNeonGreen

    Plasmid

    #99134

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CMV-Nme2ABE8e

    Plasmid

    #189928

    Purpose

    Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    Nme2ABE8e
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSOUP

    Plasmid

    #165419

    Purpose

    The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

    Insert

    Rep A
    

    Use

    Helper plasmid required for replication for all p…

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TFORF1207

    Plasmid

    #144089

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CASZ1 (CASZ1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FLAG-Pol-II WT

    Plasmid

    #35175

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTW2082

    Plasmid

    #236576

    Purpose

    ISYmu1 PDS3 gRNA12 TRV2 Architecture_B

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    ISYmu1 TnpB
    

    Expression

    Plant

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-MMLVgag-3xNES-ABE8e

    Plasmid

    #181751

    Purpose

    MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

    Depositor

    David Liu

    Article

    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

    Insert

    MMLVgag-3xNES-ABE8e
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. plRL117

    Plasmid

    #163635

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Anti-PSD-95 [K28/43R]

    Plasmid

    #128618

    Purpose

    Mammalian Expression Plasmid of anti-PSD-95 (Human). Derived from hybridoma K28/43.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    Anti-PSD-95 (Human) recombinant mouse monoclonal antibody (DLG4 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-Camui-CR

    Plasmid

    #40256

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Camuiα-CR
    

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUltra-5HTP

    Plasmid

    #178042

    Purpose

    Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    Modified ScTrpRS for 5HTP incoporation
    suppressor tRNA for 5HTP incorporation
    

    Expression

    Bacterial

    Mutation

    T107C, P254T, C255A

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBABE-puro-DN-hTERT

    Plasmid

    #1775

    Depositor

    Bob Weinberg

    Article

    Hahn et al Nat Med 1999 Oct;5(10):1164-70.

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Dominant negative, 2 base pair mutation, catalyti…

    Available Since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLX_TRC311

    Plasmid

    #113668

    Purpose

    Gateway destination vector for lentiviral expression

    Depositor

    John Doench

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP Kindlin2

    Plasmid

    #105305

    Purpose

    To study effect of Kindlin 2 on Cell/ECM adhesion

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGP-AAV-syn-jGCaMP8s-WPRE

    Plasmid

    #162374

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-TrxRFP2

    Plasmid

    #172322

    Purpose

    an improved fluorescent biosensor for thioredoxin redox

    Depositor

    Huiwang Ai

    Article

    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

    Insert

    TrxRFP2, an improved version from TrxRFP1
    

    Tags

    His-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDZ2FE_Champ1_1-812

    Plasmid

    #105675

    Purpose

    Expresses Flag-tagged full-length human Champ1 protein

    Depositor

    Thanos Halazonetis

    Insert

    Champ1 (CHAMP1 Human)
    

    Tags

    2X Flag tag

    Expression

    Mammalian

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCAG-Cre-IRES2-GFP

    Plasmid

    #26646

    Purpose

    Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

    Depositor

    Anjen Chenn

    Article

    Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    GFP and IRES2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pFnCpfAb-apr

    Plasmid

    #214198

    Purpose

    Expresses FnCpf1 and RecAb recombination system for genome editing in Acinetobacter baumannii

    Depositor

    Yu Wang

    Article

    Wang et al Curr Issues Mol Biol. 2024 Jan 5;46(1):5

    Insert

    FnCpf1
    

    Use

    CRISPR

    Promoter

    tac promoter

    Available Since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLV-Golgi eGFP

    Plasmid

    #79809

    Purpose

    To express and target GFP into the Golgi apparatus using lentiviruses

    Depositor

    Pantelis Tsoulfas

    Insert

    Golgi eGFP (B4GALT1 Human)
    

    Use

    Lentiviral

    Tags

    Fusion of the first 62 amino acids of the human b…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pH2B_mCherry_IRES_neo3

    Plasmid

    #21044

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    pH2B
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. SNAP-23

    Plasmid

    #186686

    Purpose

    Expresses SNAP-23 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    SNAP23
    

    Tags

    GST and thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. 20XUAS IVS CsChrimson tdtomato_tr

    Plasmid

    #111544

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chrimson_tdtomato_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. prhaBAD-anti-mouse-Nb-Hia5

    Plasmid

    #239626

    Purpose

    Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

    Depositor

    Aaron Straight

    Article

    Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

    Insert

    Hia5
    

    Tags

    6HIS, MBP, TEV, and anti-Mouse Nanobody

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available Since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAAVS1-PDi-CRISPRn

    Plasmid

    #73500

    Purpose

    Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    Cas9
    rtTA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CAG and TRE

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. pBlueSKMimRNA

    Plasmid

    #102535

    Purpose

    Template for synthesis of Minos transposase mRNA

    Depositor

    Michalis Averof

    Article

    Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

    Insert

    Minos transposase mRNA
    

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pHLSec2-FN-YPet

    Plasmid

    #65421

    Purpose

    Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7

    Depositor

    Harold Erickson

    Article

    Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9

    Insert

    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
    

    Tags

    YPet

    Expression

    Mammalian

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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