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177,170 results

Showing: 6151 - 6200 of 177170 results

  2. pTriEx-RhoA-wt_mScarlet-i_SGFP2

    Plasmid
    #85071
    Purpose
    Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity
    Depositor
    Dorus Gadella
    Publication
    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
    Tags
    6xHis and mScarlet-i and SGFP2
    Expression
    Bacterial, Insect, and Mamm…
    Promoter
    CMV
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    85071-plasmid-map-sequence-id-200384

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  3. pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid
    #85006
    Purpose
    For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.
    Depositor
    Takuji Iwasato
    Publication
    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-ires-tTA
    Expression
    Mammalian
    Available since
    Nov. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    85006-plasmid-map-sequence-id-167292

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  10. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Publication
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    71288-plasmid-map-sequence-id-166343

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  13. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

    Plasmid
    #71293
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Publication
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Tags
    FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    71293-plasmid-map-sequence-id-127936

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  17. Ii-Str_ssSBP-EGFP

    Plasmid
    #65277
    Purpose
    to synchronize transport of secretory soluble GFP from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP
    Tags
    IL-2 signal sequence and Streptavidin Binding Pr…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65277-plasmid-map-sequence-id-110485

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  19. GFP-ASH1L

    Plasmid
    #65369
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ASH1L (ASH1L Human)
    Tags
    EmGFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    65369-plasmid-map-sequence-id-437367

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  21. pHSN6I01

    Plasmid
    #50587
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    50587-plasmid-map-sequence-id-75659

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  24. pRGE31

    Plasmid
    #50929
    Purpose
    Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoter
    Depositor
    Yinong Yang
    Publication
    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Rice snoRNA U3 and dual 35S promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50929-plasmid-map-sequence-id-78025

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  27. pRS316-RGR-GFP

    Plasmid
    #51056
    Purpose
    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.
    Depositor
    Yunde Zhao
    Publication
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    RGR-GFP
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Promoter
    Yeast ADH1 promoter
    Available since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    51056-plasmid-map-sequence-id-77326

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Showing: 6151 - 6200 of 177170 results