19,190 results

1. pRRLSIN.cPPT.PGK-GFP.WPRE

   Plasmid

   #12252

   Purpose

   3rd generation lentiviral backbone

   Depositor

   Didier Trono

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-BAZ1B

   Plasmid

   #65372

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BAZ1B (BAZ1B Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ai139 (TIT2L-GFP-ICL-TPT) targeting vector

   Plasmid

   #114426

   Purpose

   Target a Cre-dependent GFP and a tdTomato-P2A-tTA2 expression cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GFP, tdTomato, tTA2,
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   TRE2, CAG

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-TA-ERN

   Plasmid

   #80474

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Halo-TNFa-RUSH

   Plasmid

   #166901

   Purpose

   RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-Halo

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   HaloTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCasSA

   Plasmid

   #98211

   Purpose

   CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

   Depositor

   Quanjiang Ji

   Article

   Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-N-Flag-NLRP3

   Plasmid

   #75127

   Purpose

   Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF5B-FRT-GFP-αTAT1

   Plasmid

   #27099

   Depositor

   Maxence Nachury

   Article

   Shida et al Proc Natl Acad Sci U S A. 2010 Nov 10. (

   Insert

   α-Tubulin K40 acetyltransferase1 (ATAT1 Human)
   

   Use

   Tags

   GFP, S-tag, and TEV site

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-ORF-V2

   Plasmid

   #73638

   Purpose

   Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Use

   Tags

   Venus fragment 2 (V2)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-FLEX-Arch-GFP

    Plasmid

    #22222

    Purpose

    Cre-dependent viral expression of Arch-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Has Service

    AAV1 and AAV9

    Insert

    Arch
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CAG

    Available since

    Jan. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-mCherry-Cre-blast

    Plasmid

    #179390

    Purpose

    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    mCherry (P2A) Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIP-SFFV-mtagBFP-2A

    Plasmid

    #102585

    Purpose

    Control lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoter

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-R-GECO1

    Plasmid

    #32444

    Purpose

    Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-U6-sgRNA-hSyn-mCherry

    Plasmid

    #87916

    Purpose

    sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

    Depositor

    Alex Hewitt

    Article

    Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-32a(+)-TGFB3

    Plasmid

    #120288

    Purpose

    Produce human recombinant TGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL0527 (pDonor)

    Plasmid

    #130634

    Purpose

    Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.hSynap.iGABASnFR

    Plasmid

    #112159

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Has Service

    AAV1

    Insert

    iGABASnFR
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_PGK

    Plasmid

    #79120

    Purpose

    Lentiviral vector for constitutive expression of genes (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-GABARAP

    Plasmid

    #137760

    Purpose

    Expression of GABARAP

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-puro-myr-HA-Akt1

    Plasmid

    #46969

    Purpose

    lentiviral expression of myr-HA-Akt1

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCB591

    Plasmid

    #141096

    Purpose

    E. coli-Lactobacilli shuttle vector for recombineering template cloning

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV5 BRGI-Flag

    Plasmid

    #19143

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST-ORF-V1

    Plasmid

    #73637

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3 PARL-FLAG-CT wild type

    Plasmid

    #13639

    Depositor

    Luca Pellegrini

    Article

    Sik et al J Biol Chem. 2004 Apr 9. 279(15):15323-9

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pGL3-U6-sgRNA-PGK-puromycin

    Plasmid

    #51133

    Purpose

    expresses sgRNA from the U6 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSL0283 (pTnsABC)

    Plasmid

    #130633

    Purpose

    Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTnsA, VchTnsB, VchTnsC
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-His:dCas9:nls:10P3

    Plasmid

    #135982

    Purpose

    Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    dCas9 fused to 10 copies of the P3 coiled-coil
    

    Use

    CRISPR

    Tags

    His

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Ubc.Luc.IRES.Puro

    Plasmid

    #33307

    Depositor

    Linzhao Cheng

    Article

    Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSimple-miR302/367

    Plasmid

    #98748

    Purpose

    Episomal reprogramming

    Depositor

    Sara Howden, James Thomson

    Article

    Howden et al Stem Cell Reports. 2015 Dec 8;5(6):1109-

    Insert

    miR302/367 (miRNA cluster) (MIR302A, MIR367 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HP4_M13

    Plasmid

    #120340

    Purpose

    Helper plasmid for production of phagemid ssDNA, based on m13mp18

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

    Insert

    Coding part of the M13 phage genome
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native phage promoters

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge