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Showing: 5001 - 5040 of 158806 results

  5. 5aa SunTag VP64 EVD

    Plasmid
    #115483
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    115483-plasmid-map-sequence-id-238821

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  11. pSecUAG_UTu1D

    Plasmid
    #233867
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant
    Depositor
    Natalie Krahn, Dieter Söll
    Inserts
    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG) and …
    Promoter
    EM7, araBAD, and native As SelD
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233867-plasmid-map-sequence-id-471093

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  14. pLAS2w.Phyg-HAVCR1

    Plasmid
    #232669
    Purpose
    expresses HAVCR1 in mammalian cells
    Depositor
    Chen-Yang Shen
    Insert
    HAVCR1 (HAVCR1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    232669-plasmid-map-sequence-id-464304

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  16. MZB117

    Plasmid
    #228481
    Purpose
    "Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    GST (glutathione S-transferase)
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228481-plasmid-map-sequence-id-454022

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  17. pDM069

    Plasmid
    #216821
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold2
    Use
    Tags
    RIPGLIN and VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216821-plasmid-map-sequence-id-430130

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  19. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid
    #216695
    Purpose
    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)
    Depositor
    Franck Perez
    Article
    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s
    Inserts
    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    Use
    Tags
    FIREtag, Streptavidin Binding Protein (SBP), and …
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216695-plasmid-map-sequence-id-429706

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  20. DRD1-DuET

    Plasmid
    #213227
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    DRD1 (DRD1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213227-plasmid-map-sequence-id-431800

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  21. PB-3xCTCF-TetO

    Plasmid
    #213023
    Purpose
    piggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposase
    Depositor
    Luca Giorgetti
    Article
    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do
    Insert
    3xCTCF-TetO
    Use
    Mouse Targeting; Piggybase integration (transposo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    213023-plasmid-map-sequence-id-428697

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  22. p663-UBC-miniTurbo-V5-RIOK1_IDG-K

    Plasmid
    #214464
    Purpose
    Gateway destination clone of RIOK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-RIOK1 (RIOK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214464-plasmid-map-sequence-id-423214

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  24. pLKO.1 LARP7-FLAG 7SK

    Plasmid
    #200733
    Purpose
    Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK
    Depositor
    Claudio Alarcón
    Insert
    LARP7 and 7SK (LARP7 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    200733-plasmid-map-sequence-id-401803

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  27. DCAF1:PBC020-D05:C246337

    Plasmid
    #183974
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    DCAF1:PBC020-D05:C246337 (DCAF1 Human)
    Use
    Baculovirus expresssion
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183974-plasmid-map-sequence-id-361427

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  31. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

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  32. pBR322-TIMER

    Plasmid
    #103056
    Purpose
    medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)
    Depositor
    Dirk Bumann
    Article
    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1
    Insert
    TIMER-bac
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mutations: S4T and S179T
    Promoter
    constitutive ybaJp promoter
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    103056-plasmid-map-sequence-id-199055

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  37. phage UbiC NLS HA stdMCP stdHalo

    Plasmid
    #104999
    Purpose
    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling
    Depositor
    Jeffrey Chao
    Article
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    stdMCP-stdHalo
    Use
    Lentiviral
    Tags
    HA tag, NLS, Two synonymous copies of the Halo-ta…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    UbiC
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    104999-plasmid-map-sequence-id-202896

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Showing: 5001 - 5040 of 158806 results