Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 16,885 results for: Por

Showing: 6351 - 6400 of 16885 results

  9. THI VP64 pGAP T-

    Plasmid
    #161487
    Purpose
    Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Non targeting control NTC)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pGAP, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161487-plasmid-map-sequence-id-326158

    Enlarge

  18. pCI-TPI_WT-xrRNA-4H

    Plasmid
    #108368
    Purpose
    Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108368-plasmid-map-sequence-id-209437

    Enlarge

  19. pMG

    Plasmid
    #138264
    Purpose
    Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Inserts
    eGFP flanked by iCas9 sites
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tef1
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138264-plasmid-map-sequence-id-273626

    Enlarge

  22. pCI-globin_del5UTR_PTC39-xrRNA-4H

    Plasmid
    #108367
    Purpose
    Expresses beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
    Use
    Tags
    Expression
    Mammalian
    Mutation
    PTC39
    Promoter
    CMV
    Available Since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    108367-plasmid-map-sequence-id-209435

    Enlarge

  23. pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H

    Plasmid
    #108376
    Purpose
    FRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108376-plasmid-map-sequence-id-208352

    Enlarge

  26. pU6_T7_INT[6xminimal_Puf1]

    Plasmid
    #107608
    Purpose
    Transient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoter
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    6x minimal Puf1 motif
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107608-plasmid-map-sequence-id-207490

    Enlarge

  28. pIhh742bp-Luc

    Plasmid
    #53605
    Purpose
    Reporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays
    Depositor
    Xiangli Yang
    Article
    Wang et al Development. 2009 Dec;136(24):4143-53. d
    Insert
    Ihh promoter
    Use
    Luciferase; Reporter
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 30, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pcDNA3-PKAsub-YPet-NES

    Plasmid
    #138203
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    April 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    138203-plasmid-map-sequence-id-307404

    Enlarge

  31. pcDNA3-JNKsubDD-YPet-NES

    Plasmid
    #84633
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    JNKsubDD-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84633-plasmid-map-sequence-id-176152

    Enlarge

  35. XW20

    Plasmid
    #65836
    Purpose
    Punc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)
    Depositor
    Kang Shen
    Article
    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi
    Insert
    OR74A quinic acid utilization activator (qa-1F)
    Use
    Tags
    Expression
    Worm
    Mutation
    The endogenous SphI, KpnI and SacI sites in QF ar…
    Promoter
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65836-plasmid-map-sequence-id-120566

    Enlarge

  43. GFP-CmTMC1truncated

    Plasmid
    #154908
    Purpose
    For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.
    Depositor
    Motoyuki Hattori
    Article
    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d
    Insert
    GFP-CmTMC1truncated
    Use
    Tags
    8histidine-EGFP
    Expression
    Insect
    Mutation
    Truncations of the residues 1-243 and 843-969
    Promoter
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154908-plasmid-map-sequence-id-310626

    Enlarge

  44. GFP-MuTMC2truncated

    Plasmid
    #154909
    Purpose
    For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.
    Depositor
    Motoyuki Hattori
    Article
    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d
    Insert
    GFP-MuTMC2truncated
    Use
    Tags
    8histidine-EGFP
    Expression
    Insect
    Mutation
    Truncations of the residues 1-179 and 691-716
    Promoter
    Available Since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    154909-plasmid-map-sequence-id-306870

    Enlarge

Showing: 6351 - 6400 of 16885 results