19,770 results

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pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

Plasmid

#164510

Purpose

Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.hSynap.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244092) instead of this plasmid.

Depositor

Loren Looger

Article

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Has Service

AAV9

Insert

(Cyto)iGlucoSnFR-mRuby2

Use

AAV

Mutation

Synthetic Construct

Promoter

Synapsin

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-Giantin_C1

Plasmid

#189773

Purpose

Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-Giantin

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 NFAT3

Plasmid

#10961

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

Insert

NFAT3 (NFATC4 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

Plasmid

#138152

Purpose

Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

eGFP-MCS

Use

Lentiviral

Tags

2PEST AND NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

Plasmid

#55639

Purpose

Flp-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde

Insert

hChR2(H134R)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKT-PH[R25C]-GFP

Plasmid

#18837

Depositor

Craig Montell

Article

Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

Insert

AKT-PH domain (AKT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

R25C

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual-M2

Plasmid

#135584

Purpose

High eukaryotic dual protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Article

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Expression

Insect

Promoter

polyhedrin of AcMNPV

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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M13KO7-ΔPS-ΔgeneIII

Plasmid

#134351

Purpose

Helper phage for M13 phage production

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Helper phage gene

Expression

Bacterial

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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double cut NH-donor

Plasmid

#83576

Purpose

eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

eGFP

Use

Nhej donor

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-NbALFA

Plasmid

#159987

Purpose

For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mCherry-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mCherry and NbALFA

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-ACEmax

Plasmid

#139105

Purpose

Mammalian Target-ACEmax expression vector (pSI-625)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLOX-TERT-iresTK

Plasmid

#12245

Purpose

2nd generation lentiviral vector for reversible immortalization of human primary cells

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

TERT, HSV1-TK (TERT Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-minCMV-mCherry

Plasmid

#27970

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

mCherry

Use

AAV

Expression

Mammalian

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-2200

Plasmid

#31062

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-2200 to -1 of P2 HNF4A promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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iRFP-P4M-SidM

Plasmid

#51470

Purpose

Biosensor for PtdIns4P

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Insert

P4M-SidM(546-647)

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 S310F

Plasmid

#40991

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

S310F

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pET-21a_3xNLS_SpCas9_protein_expression

Plasmid

#114365

Purpose

Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

Depositor

Scot Wolfe

Article

Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

Insert

3xNLS_SpCas9

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO1

Plasmid

#53138

Purpose

E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

SUMO1 (SUMO1 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-97)

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-mScarlet-WPRE

Plasmid

#130994

Purpose

AAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

Plasmid

#164611

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Expression

Mammalian

Mutation

S383T

Promoter

CMV

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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HLA_A2402

Plasmid

#165010

Purpose

Expression of HLA-A*24:02 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-A*24:02 (HLA-A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-Oct4

Plasmid

#16352

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)

Expression

Bacterial

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pSynSRE-T-Luc

Plasmid

#60444

Purpose

Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

Depositor

Timothy Osborne

Article

Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

Insert

HMG-CoA synthase promoter (-324/-225)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-BLIMP-1-pcDNA3.1-

Plasmid

#52518

Purpose

expresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blimp1 (PRDM1 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-dCas9-p300-P2A-PuroR

Plasmid

#83889

Purpose

Lentiviral Sp dCas9-p300-P2A-PuroR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
pac from Streptomyces alboniger

Use

CRISPR and Lentiviral

Tags

Flag and nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EFS

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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Lentiviral-S-tdTomato

Plasmid

#112579

Purpose

Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

Depositor

Jeffrey Rothstein

Article

Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

Insert

NLS-tdTomato-NES

Use

Lentiviral

Tags

nuclear export sequence and nuclear localization …

Expression

Mammalian

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-CytERM-mCherry-(F30-2xPepper)10

Plasmid

#129403

Purpose

CytERM-mCherry mRNA reporter tagged with (F30-2xPepper)10

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

CytERM-mCherry-(F30-2xPepper)10

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_WT

Plasmid

#98651

Purpose

Expresses MBP-tagged full length FUS

Depositor

Nicolas Fawzi

Article

Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

Plasmid

#138315

Purpose

Lenti expression of mTgBFP2

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

mTAGBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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p44K

Plasmid

#45800

Depositor

Luc Ponchon

Article

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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