We narrowed to 11,711 results for: pcDNA

1. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)

   Plasmid

   #204696

   Purpose

   This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-NUT(deltaMIN) (BRD4, NUTM1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   deletion of the amino acids 355-505 of NUT

   Promoter

   CMV

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)

   Plasmid

   #204695

   Purpose

   This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-NUT(MIN) (BRD4, NUTM1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   deletion of majority of NUT, retaining only amino…

   Promoter

   CMV

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-MS2-HA-HsNot4_275-575-del377-402_AG

   Plasmid

   #148753

   Purpose

   Mammalian Expression of HsNot4_275-575-del377-402

   Depositor

   Elisa Izaurralde

   Article

   Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

   Insert

   HsNot4_275-575-del377-402 (CNOT4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-MS2-HA-HsNot4_275-575-del377-424_AG

   Plasmid

   #148754

   Purpose

   Mammalian Expression of HsNot4_275-575-del377-424

   Depositor

   Elisa Izaurralde

   Article

   Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

   Insert

   HsNot4_275-575-del377-424 (CNOT4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-MS2-HA-HsNot4_275-575-del400-428_AG

   Plasmid

   #148755

   Purpose

   Mammalian Expression of HsNot4_275-575-del400-428

   Depositor

   Elisa Izaurralde

   Article

   Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

   Insert

   HsNot4_275-575-del400-428 (CNOT4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-MS2-HA-HsSmg5-del133-155-G120E_T

   Plasmid

   #147554

   Purpose

   Mammalian Expression of HsSmg5-del133-155-G120E

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg5-del133-155-G120E (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-MS2-HA-HshnRNPH1_185-449-del291-361_Z

   Plasmid

   #148123

   Purpose

   Mammalian Expression of HshnRNPH1_185-449-del291-361

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPH1_185-449-del291-361 (HNRNPH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3_SP-HTR2A-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194369

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3_SP-AVPR2-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194373

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-AVPR2-V2R-NTEV-TCS-GV-2xHA (AVPR2 Synthetic, Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3_SP-DRD1-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194361

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    SP-DRD1-V2R-NTEV-TCS-GV-2xHA (DRD1 Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3_SP-DRD2-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194365

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    SP-DRD2-V2R-NTEV-TCS-GV-2xHA (DRD2 Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-EGFP MASTL E167D (siRNA resistant)

    Plasmid

    #191013

    Purpose

    Expresses EGFP-tagged MASTL with E167D mutation and resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

    Depositor

    Johanna Ivaska

    Article

    Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Microtubule-associated serine/threonine kinase like (MASTL Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E167D

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.2-V5-DEST-hSFRP1(aa181-314)-his

    Plasmid

    #189549

    Purpose

    Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.

    Depositor

    Haian Fu

    Insert

    truncated secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    his

    Expression

    Mammalian

    Mutation

    contains aa181-314

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M

    Plasmid

    #140454

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, tyrosine 616 to methioni…

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

    Plasmid

    #140455

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, leucine 624 to asparagine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

    Plasmid

    #140456

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, alanine 634 to asparagine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

    Plasmid

    #140452

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, isoluecine 578 to alanine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V

    Plasmid

    #140453

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, phenylalanine 588 to val…

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry

    Plasmid

    #181855

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Histidine 33 in DnaJB1 changed to glutamine.

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA6-5'UTR(mtIF3)-Dendra2-3'UTR(mtIF3)

    Plasmid

    #182369

    Purpose

    Dendra2-based translation reporter

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Insert

    mtIF3 (Mtif3 Mouse)
    

    Use

    Tags

    Pal-Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1(+)-tdMCP2-GyG-(GGS)X6-HaloTag C261

    Plasmid

    #171434

    Purpose

    Expression of tdMCP2-GyG-(GGS)X6-HaloTag C261 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    tdMCP2-GyG-(GGS)X6-HaloTag C261
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5 FRT-Hygro hTERT dEx2-nanoLuc

    Plasmid

    #171056

    Purpose

    hTERT dEx2-expressing splicing minigene

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    hTERT dEx2-nanoLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDNA4 3xFLAG Halo R119A K120A nsp1

    Plasmid

    #175428

    Purpose

    Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 R119A K120A
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants R119A K120A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA4 3xFLAG Halo E36A E37A nsp1

    Plasmid

    #175429

    Purpose

    Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 E36A E37A
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants E36A E37A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G

    Plasmid

    #169960

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    F370G

    Promoter

    CMV

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3HA-AMOT p130∆PPxY1(E104K/E105K)

    Plasmid

    #171130

    Purpose

    expresses AMOT(E104K/E105K) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    Angiomotin p130 E104K/E105K (AMOT Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    changed glutamic acid residues 104 and 105 to lys…

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A

    Plasmid

    #169955

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A, R394E

    Promoter

    CMV

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A

    Plasmid

    #169957

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A, F405G

    Promoter

    CMV

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368G

    Plasmid

    #169959

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    L368G

    Promoter

    CMV

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394E

    Plasmid

    #169961

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    R394E

    Promoter

    CMV

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405G

    Plasmid

    #170119

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed).

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    F405G

    Promoter

    CMV

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A

    Plasmid

    #169951

    Purpose

    Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E274R, S297A

    Promoter

    CMV

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE

    Plasmid

    #166805

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The LL1193E mutant abolishes FL-NLRP1 association with DPP9 at interface I.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    LL193EE

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA 3.1 LIC 6A NLRP1-FLAG K1277E

    Plasmid

    #166808

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K1277E

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA 3.1 LIC 6A NLRP1-FLAG H1276G

    Plasmid

    #166807

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H1276G

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S RK96EE

    Plasmid

    #166797

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. RK96EE mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    RK96EE

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL102EE

    Plasmid

    #166799

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. LL102EE mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    LL102EE

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S D808N

    Plasmid

    #166802

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. The D808N mutant is catalytically dead.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    D808N

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA 3.1 LIC 6A NLRP1-FLAG P1214R

    Plasmid

    #166806

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The P1214R patient mutant abolishes NLRP1-CT association with DPP9 at interface II.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    P1214R

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

    Plasmid

    #164013

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R464E

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.1 6B CARD8 UPA-CARD-mCherry R495E

    Plasmid

    #164016

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R495E

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1 6B CARD8 UPA-CARD-mCherry Y527A

    Plasmid

    #164018

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Y527A

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A

    Plasmid

    #162639

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids K174, R179, and K182 mutated to alani…

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

    Plasmid

    #163991

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG W1460A

    Plasmid

    #163989

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    W1460A

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG Y1445A

    Plasmid

    #163987

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y1445A

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1427E

    Plasmid

    #163986

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R1427E

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1414R

    Plasmid

    #163985

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E1414R

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1411R

    Plasmid

    #163984

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E1411R

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG D1401R

    Plasmid

    #163983

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R1401R

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge